LOCUS       sequence-271608-        1827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Casler JC, Glick BS
  TITLE     A microscopy-based kinetic analysis of yeast vacuolar protein 
            sorting.
  JOURNAL   Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844.
  PUBMED    32584255
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-25"; volume: "9. pii: 56844. doi"; pages: " 
            10.7554/eLife.56844"
COMMENT     Sequence Label: YIplac211-Mup1-mScarlet2I
FEATURES             Location/Qualifiers
     source          1..1827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggacgtgaag gtgtgttgcc cttttctaat ttctttgcct cttctaagcc attcaactcc
       61 ccaatggttg gtttgtttca acattttatt gtttgtacag taaccatttt agccccacct
      121 ccaggtgatg catatttgct agtccaaaat ttaatttcct acccaatgaa tattattaat
      181 tttgccatta gcgcgggttt gctttggata tactggcaac gtagacaagg taaaattgaa
      241 tggaacccac caattaaggc cggtgttttt gttacgggat ttttcacatt atccaattta
      301 tacctgatta ttgctcctta cgttccacct tcaaatggcg agtcggtgta ttcaagcatg
      361 ccatactgga tccactgtgt aattgcttgg ggtattttct tctttggtgg tgtttactac
      421 gttgtttggg cccaattatt gccaagatgg ggccactaca aattggtttc caaggatgtg
      481 cttggtgaag atgggttctg gagagtcaaa attgccaaag tttatgatga cactattggc
      541 gatgtcgata cacaagaaga cggcgttatt gaaacgaata taatcgaaca ttacaaaagt
      601 gaacaagaaa aatcgctgaa agatccaact agtatggata gtacagaagc agttataaag
      661 gaattcatga gattcaaagt ccacatggaa ggtagtatga acggtcacga attcgaaata
      721 gaaggtgaag gtgaaggtag accatatgaa ggtactcaaa cagctaaatt gaaggttact
      781 aaaggtggtc cattgccatt ttcttgggat atcttgtctc cacaattcat gtacggttca
      841 agagctttta ttaaacatcc agcagatatt ccagattact acaagcaatc atttccagaa
      901 ggttttaaat gggaaagagt tatgaacttc gaagatggtg gtgcagttac tgttacacaa
      961 gatacttctt tggaagatgg tacattgatc tataaggtta aattgagagg tacaaatttt
     1021 ccaccagatg gtccagttat gcaaaagaaa actatgggtt gggaagcatc tacagaaaga
     1081 ttgtacccag aagatggtgt tttgaagggt gacatcaaga tggcattgag attgaaggat
     1141 ggtggtagat atttggctga tttcaagact acatacaagg ctaagaaacc agttcaaatg
     1201 ccaggtgctt acaacgttga tagaaaattg gatatcactt ctcataacga agattacaca
     1261 gttgttgaac aatacgaaag aagtgaaggt agacatagta caggtggtag tggtgctagc
     1321 taagcggccg cccaaaaagg tacttaccat ctcatctcga ttcttataat cacgtatgaa
     1381 cattcttata attgcatttt ctttatacaa aatatatagg taccaaacct cattataatt
     1441 tctgccattt aatagtttaa gatgctattc cgtttcgtat tttttcaatt tttataccta
     1501 ctattgcgcg aaatcaaaat agcatactaa gaaaaagcaa tatcatgaat tgctgatgag
     1561 atagattcaa attggaatta actaaatcta tcagcaacgc tataaacaca gataagcgct
     1621 ctataaaatc gtttgtctaa cgttttatat agttctttat tgctccctta ccctctttcc
     1681 gtcaatagtg attgttgtct cgggaaagct ttttttttta ttaatcaaag gcccaattga
     1741 gttacaactg aaattaagct gagttggcgg aacagtgtgg tattagcgaa gggaaaatct
     1801 gtgaagtgcc ctagctacaa aacagag
//
