LOCUS       sequence-271609-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Casler JC, Glick BS
  TITLE     A microscopy-based kinetic analysis of yeast vacuolar protein 
            sorting.
  JOURNAL   Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844.
  PUBMED    32584255
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-25"; volume: "9. pii: 56844. doi"; pages: " 
            10.7554/eLife.56844"
COMMENT     Sequence Label: 
            YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..756
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     complement(262..282)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     480..499
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     674..697
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             769..1089
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             769..1089
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             769..1089
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     CDS             769..1089
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atgaggcagg tttggttctc ttggattgtg ggattgttcc tatgtttttt caacgtgtct
       61 tctgctgcgc cggtgaacac caccgatagc actgagaacg tcatcaagcc cttcatgcgc
      121 ttcaaggtgc acatggaggg ctcagtaaat ggtcatgagt tcgagatcga gggcgagggc
      181 gagggcaagc cctacgaggg cacccagacc gccaagctgc aggtgaccaa gggcggcccc
      241 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg
      301 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      421 caggacggca ccttcatcta ccacgtgaag ttcatcggcg tgaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac tctgggctgg gagccctcca ccgagcgcct gtacccccgc
      541 gacggcgtgc tgaagggcga gatccacaag gcgctgaagc tgaagggcgg cggccactac
      601 ctggtggagt tcaagtcaat ctacatggcc aagaagcccg tgaagctgcc cggctactac
      661 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccgt ggtggagcag
      721 tacgagcgcg ccgaggcccg ccaccacctg ttccagggat cctctagagg agtgcaggtg
      781 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac cgttgtggtg
      841 cactacaccg ggatgcttga agatggaaag aaactagatt cctcccggga cagaaacaag
      901 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
      961 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     1021 actgggcacc caggcgttat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     1081 aaactggaag gatctcagag accactggga ggatcctaa
//
