LOCUS       sequence-271615-        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo (without an ER export 
            signal) for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pEF-pIgH-ESCargo*
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     join(1..45,128..139)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(1..46,129..139)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             140..811
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     complement(317..337)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     535..554
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     729..752
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             824..1144
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggtaa ggggttaaca
       61 gtagcaggct tgaggtctgg acatatatat gggtgacaat gacatccact ttgcctttct
      121 ctccacaggc gcgcactccg atagcactga gaacgtcatc aagcccttca tgcgcttcaa
      181 ggtgcacatg gagggctcag taaatggtca tgagttcgag atcgagggcg agggcgaggg
      241 caagccctac gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc
      301 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
      361 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
      421 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      481 cggcaccttc atctaccacg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
      541 aatgcagaag aagactctgg gctgggagcc ctccaccgag cgcctgtacc cccgcgacgg
      601 cgtgctgaag ggcgagatcc acaaggcgct gaagctgaag ggcggcggcc actacctggt
      661 ggagttcaag tcaatctaca tggccaagaa gcccgtgaag ctgcccggct actactacgt
      721 ggactccaag ctggacatca cctcccacaa cgaggactac accgtggtgg agcagtacga
      781 gcgcgccgag gcccgccacc acctgttcca gggaggatcc agaggagtgc aggtggaaac
      841 catctcccca ggagacgggc gcaccttccc caagcgcggc cagaccgttg tggtgcacta
      901 caccgggatg cttgaagatg gaaagaaact agattcctcc cgggacagaa acaagccctt
      961 taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat
     1021 gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg
     1081 gcacccaggc gttatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact
     1141 ggaatag
//
