LOCUS       sequence-271617-        1165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pEF-pIgH-ESCargo(FTV,Orange)
FEATURES             Location/Qualifiers
     source          1..1165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     join(1..45,128..139)
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             join(1..46,129..139)
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             158..829
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLVEFKTIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     primer_bind     553..572
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     747..770
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             842..1162
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggtaa ggggttaaca
       61 gtagcaggct tgaggtctgg acatatatat gggtgacaat gacatccact ttgcctttct
      121 ctccacaggc gcgcactcct ttaccgtgaa caccaccgat agcactgaga acgtcatcaa
      181 gcccttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      241 cgagggcgag ggcgagggca agccctacga gggcacccag accgccaagc tgcaggtgac
      301 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca cctacggctc
      361 caaggcctac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
      421 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      481 ggactcctcc ctgcaggacg gcaccttcat ctaccacgtg aagttcatcg gcgtgaactt
      541 cccctccgac ggccccgtaa tgcagaagaa gactctgggc tgggagccct ccaccgagcg
      601 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggcgctga agctgaaggg
      661 cggcggccac tacctggtgg agttcaagac aatctacatg gccaagaagc ccgtgaagct
      721 gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac
      781 cgtggtggag cagtacgagc gcgccgaggc ccgccaccac ctgttccagg gaggatccag
      841 aggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca agcgcggcca
      901 gaccgttgtg gtgcactaca ccgggatgct tgaagatgga aagaaactag attcctcccg
      961 ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga
     1021 agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta
     1081 tgcctatggt gccactgggc acccaggcgt tatcccacca catgccactc tcgtcttcga
     1141 tgtggagctt ctaaaactgg aatag
//
