LOCUS       sequence-271619-        2442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo for mammalian cells and a 
            Golgi marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pcDNA-GalNAc-T2-GFP-ESCargo(FTV)
FEATURES             Location/Qualifiers
     source          1..2442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1732..2439
                     /codon_start=1
                     /product="photostable monomeric superfolder GFP (Valbuena 
                     et al., 2020)"
                     /label=msGFP2
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITGGSGGS"
     primer_bind     complement(1774..1795)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2382..2403
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgaggcggc gctcgaggat gctgctctgc ttcgccttcc tgtgggtgct gggaatcgcc
       61 tactacatgt actctggagg aggatctgcg ctggccggag gagcgggagg cggagccgga
      121 aggaaggagg actggaatga aattgacccc attaaaaaga aagaccttca tcacagcaat
      181 ggagaagaga aagcacaaag catggagacc ctccctccag ggaaagtacg gtggccagac
      241 tttaaccagg aagcttatgt tggagggacg atggtccgct ccgggcagga cccttacgcc
      301 cgcaacaagt tcaaccaggt ggagagtgat aagcttcgaa tggacagagc catccctgac
      361 acccggcatg accagtgtca gcggaagcag tggcgggtgg atctgccggc caccagcgtg
      421 gtgatcacgt ttcacaatga agccaggtcg gccctactca ggaccgtggt cagcgtgctt
      481 aagaaaagcc cgccccatct cataaaagaa atcatcttgg tggatgacta cagcaatgat
      541 cctgaggacg gggctctctt ggggaaaatt gagaaagtgc gagttcttag aaatgatcga
      601 cgagaaggcc tcatgcgctc acgggttcgg ggggccgatg ctgcccaagc caaggtcctg
      661 accttcctgg acagtcactg cgagtgtaat gagcactggc tggagcccct cctggaaagg
      721 gtggcggagg acaggactcg ggttgtgtca cccatcatcg atgtcattaa tatggacaac
      781 tttcagtatg tgggggcatc tgctgacttg aagggcggtt ttgattggaa cttggtattc
      841 aagtgggatt acatgacgcc tgagcagaga aggtcccggc aggggaaccc agtcgcccct
      901 ataaaaaccc ccatgattgc tggtgggctg tttgtgatgg ataagttcta ttttgaagaa
      961 ctggggaagt acgacatgat gatggatgtg tggggaggag agaacctaga gatctcgttc
     1021 cgcgtgtggc agtgtggtgg cagcctggag atcatcccgt gcagccgtgt gggacacgtg
     1081 ttccggaagc agcaccccta cacgttcccg ggtggcagtg gcactgtctt tgcccgaaac
     1141 acccgccggg cagcagaggt ctggatggat gaatacaaaa atttctatta tgcagcagtg
     1201 ccttctgcta gaaacgttcc ttatggaaat attcagagca gattggagct taggaagaaa
     1261 ctcagctgca agcctttcaa atggtacctt gaaaatgtct atccagagtt aagggttcca
     1321 gaccatcagg atatagcttt tggggccttg cagcagggaa ctaactgcct cgacactttg
     1381 ggacactttg ctgatggtgt ggttggagtt tatgaatgtc acaatgctgg gggaaaccag
     1441 gaatgggcct tgacgaagga gaagtcggtg aagcacatgg atttgtgcct tactgtggtg
     1501 gaccgggcac cgggctctct tataaagctg cagggctgcc gagaaaatga cagcagacag
     1561 aaatgggaac agatcgaggg caactccaag ctgaggcacg tgggcagcaa cctgtgcctg
     1621 gacagtcgca cggccaagag cgggggccta agcgtggagg tgtgtggccc ggccctttcg
     1681 cagcagtgga agttcacgct caacctgcag cagggaggaa gcggaggaag catggatagc
     1741 actgagagcc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1801 ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
     1861 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1921 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1981 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcacctt caaggacgac
     2041 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2101 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2161 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     2221 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag
     2281 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2341 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2401 gtgaccgccg ccgggatcac tggaggcagt ggcggttctt ag
//
