LOCUS       sequence-271622-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo (without an ER export 
            signal) for Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pUASt-ssBiP-ESCargo*
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..54
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             519..530
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaagctgt gcattctgct cgctgtagtt gcatttgttg gtttgtcgtt gggcgattcg
       61 acagaaaatg tcatcaaacc atttatgcgg ttcaaagtac atatggaagg tagtgttaac
      121 ggtcacgaat ttgaaatcga gggcgaagga gagggcaaac catatgaagg aacacagacc
      181 gcaaaactcc aagttactaa gggtggacct ctgccgttcg cctgggatat actgtcgccc
      241 caattccagt atggaagcaa ggtgtacgta aagcacccag cggatatccc agattataaa
      301 aaactgtcgt ttccggaagg tttcaaatgg gaacgagtga tgaacttcga ggatggcgga
      361 gttgtgactg taacacaaga cagtagtctc caagacggca cattcatata tcatgtcaaa
      421 ttcattggag ttaactttcc ctcggatggt ccagtcatgc agaagaaaac cctcggttgg
      481 gagccttcga ctgaacgctt gtatccacga gacggagtat tgaagggcga aatccataag
      541 gcactgaagc tcaagggcgg aggtcactat ttggtagagt ttaagtccat atacatggca
      601 aagaagcctg taaagctccc aggatactac tacgtggata gtaaactcga tataacttcc
      661 cataatgagg attatactgt tgtagagcag tacgagaggg cagaagcgcg tcatcatctg
      721 tttcagggtt cctcccgagg tgtccaggtc gaaacgataa gtccgggaga tggtaggacg
      781 ttcccaaaga ggggtcagac cgtggtggtg cattatactg gaatgttgga agacggtaag
      841 aaattggact cgtccaggga tcgcaacaag ccgtttaaat ttatgctggg aaaacaggag
      901 gtcattcgcg gctgggaaga gggcgtcgca caaatgagtg ttggccagag ggcaaaactg
      961 acaataagtc ctgactatgc ctacggtgcg actggccatc cgggtgtcat cccgccacac
     1021 gcgacgctcg tcttcgatgt tgagctgctc aagctcgagt aa
//
