LOCUS       sequence-271623-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo for Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pUASt-ssBiP-ESCargo
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..54
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             537..548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaagctgt gcattctgct cgctgtagtt gcatttgttg gtttgtcgtt gggcgcgccc
       61 gtaaacacca ctgattcgac agaaaatgtc atcaaaccat ttatgcggtt caaagtacat
      121 atggaaggta gtgttaacgg tcacgaattt gaaatcgagg gcgaaggaga gggcaaacca
      181 tatgaaggaa cacagaccgc aaaactccaa gttactaagg gtggacctct gccgttcgcc
      241 tgggatatac tgtcgcccca attccagtat ggaagcaagg tgtacgtaaa gcacccagcg
      301 gatatcccag attataaaaa actgtcgttt ccggaaggtt tcaaatggga acgagtgatg
      361 aacttcgagg atggcggagt tgtgactgta acacaagaca gtagtctcca agacggcaca
      421 ttcatatatc atgtcaaatt cattggagtt aactttccct cggatggtcc agtcatgcag
      481 aagaaaaccc tcggttggga gccttcgact gaacgcttgt atccacgaga cggagtattg
      541 aagggcgaaa tccataaggc actgaagctc aagggcggag gtcactattt ggtagagttt
      601 aagtccatat acatggcaaa gaagcctgta aagctcccag gatactacta cgtggatagt
      661 aaactcgata taacttccca taatgaggat tatactgttg tagagcagta cgagagggca
      721 gaagcgcgtc atcatctgtt tcagggttcc tcccgaggtg tccaggtcga aacgataagt
      781 ccgggagatg gtaggacgtt cccaaagagg ggtcagaccg tggtggtgca ttatactgga
      841 atgttggaag acggtaagaa attggactcg tccagggatc gcaacaagcc gtttaaattt
      901 atgctgggaa aacaggaggt cattcgcggc tgggaagagg gcgtcgcaca aatgagtgtt
      961 ggccagaggg caaaactgac aataagtcct gactatgcct acggtgcgac tggccatccg
     1021 ggtgtcatcc cgccacacgc gacgctcgtc ttcgatgttg agctgctcaa gctcgagtaa
//
