LOCUS       sequence-271624-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo (without an ER export 
            signal) for Tetrahymena thermophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: NCVB-ssGrl1-ESCargo*
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaataaaa agctcttagt cgtcttattt ggttttttgg cattagccgc agcaaccaat
       61 gacagcactg aaaacgtaat caaaccattc atgagattta aagtccacat ggaaggaagt
      121 gtaaatggac atgagtttga gatagagggt gagggagaag gaaaacccta tgagggtacc
      181 caaacagcca agctccaagt taccaaaggt ggaccattgc ccttcgcctg ggacatcttg
      241 agcccacaat tccaatatgg tagtaaggtc tatgtaaagc accctgccga tattcccgat
      301 tataagaagt tgtcttttcc tgaaggtttt aagtgggaac gtgttatgaa ttttgaggac
      361 ggtggtgtcg taaccgtaac acaagacagc agcctccaag atggaacctt tatctatcac
      421 gttaagttca tcggtgtcaa ttttccatct gacggaccag ttatgcagaa gaagaccttg
      481 ggttgggagc cttcaaccga gcgtctctat ccaagagacg gagtattgaa gggtgaaatt
      541 cataaagcat tgaaattaaa aggtggaggt cattacttag tcgagtttaa gtcaatatat
      601 atggcaaaaa agcccgttaa gttaccagga tactactatg tcgactctaa gttagacata
      661 acaagtcaca acgaagacta tactgtcgta gagcaatacg agcgtgcaga agcccgtcat
      721 catttattcc aaggttcaag tagaggtgta caagtcgaga ccatcagtcc aggagatggt
      781 cgtacctttc ccaagagagg acaaaccgtc gttgtccatt acactggtat gttggaagac
      841 ggaaaaaagc ttgatagcag ccgtgataga aataaaccat tcaaatttat gttgggtaaa
      901 caggaagtaa ttcgtggatg ggaggagggt gtagcccaaa tgtcagtagg tcagcgtgct
      961 aaactcacta taagcccaga ttatgcttac ggtgcaactg gtcatccagg agtaatccca
     1021 cctcacgcta ccttggtatt tgacgtagag ttacttaagc ttgagtga
//
