LOCUS       sequence-271641-       10520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tet/Dox-inducible human PLOD2 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Sato K, Parag-Sharma K, Terajima M, Musicant AM, Murphy RM, Ramsey 
            MR, Hibi H, Yamauchi M, Amelio AL
  TITLE     Lysyl hydroxylase 2-induced collagen cross-link switching promotes 
            metastasis in head and neck squamous cell carcinomas.
  JOURNAL   Neoplasia. 2021 Jun 6;23(6):594-606. doi: 10.1016/j.neo.2021.05.014.
  PUBMED    34107376
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2021.05"; journalName: "Neoplasia"; date: "2021-06-6-
            6"; volume: "23"; issue: "6"; pages: "594-606"
COMMENT     Sequence Label: pRetroX-TRE3G-PLOD2_PGK-GpNLuc
FEATURES             Location/Qualifiers
     source          1..10520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1603..1952
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1647..1665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1683..1701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1719..1737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1755..1773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1791..1809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1827..1845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1891..1915
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2004..2013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4260..4313
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4314..5339
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4314..5339
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        5350..5849
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5385..5408)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5782..5801
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(5864..5866,5867..5869,5870..6580)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5864..5866,5867..5869,5870..6580)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5909..5930)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6170..6189)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6517..6538
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6596..7111
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     LTR             7226..7651
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7754..7776)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7912..7932)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7917..8246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8097..8232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8268..8287
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8368..8385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8539..9127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8619..8638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9298..10089,10090..10158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9921..9940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10159..10263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10331..10349
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10449..10520,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag
     1621 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
     1681 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
     1741 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
     1801 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta tgtcgaggta
     1861 ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg
     1921 gagcaattcc acaacacttt tgtcttatac ttacgcgtct gcaggatatc aagcttgcgg
     1981 taccgcgggc ccgatccgcg gccgccacca tgggcggctg taccgtgaag ccccagctgc
     2041 tgctgctggc cctggtgctg cacccctgga atccttgcct gggcgccgac agcgagaagc
     2101 ccagcagcat ccccaccgac aagctgctgg tcatcaccgt ggccaccaaa gagagcgacg
     2161 gcttccaccg gttcatgcag agcgccaagt acttcaacta caccgtgaag gtgctgggcc
     2221 agggcgaaga gtggagaggc ggcgacggca tcaacagcat cggcggaggg cagaaagtgc
     2281 ggctgatgaa agaagtgatg gaacactacg ccgatcagga cgatctggtg gtcatgttca
     2341 ccgagtgctt cgacgtgatc tttgccggcg gacccgagga agtgctgaag aagttccaga
     2401 aagccaacca caaggtggtg ttcgccgccg atggcatcct gtggcccgac aagagactgg
     2461 ccgacaagta ccccgtggtg cacatcggca agagatacct gaacagcggc ggcttcatcg
     2521 gctacgcccc ctacgtgaac cggatcgtgc agcagtggaa tctgcaggac aacgacgacg
     2581 accagctgtt ctacaccaag gtgtacatcg accccctgaa gcgggaggcc atcaacatca
     2641 ccctggacca caagtgcaag atcttccaga ccctgaacgg agccgtggac gaggtggtgc
     2701 tgaagttcga gaacggcaag gccagagcca agaacacctt ctacgagaca ctgcccgtgg
     2761 ccatcaacgg caacggcccc accaagatcc tgctgaacta cttcggcaac tacgtgccca
     2821 acagctggac ccaggacaac ggctgcaccc tgtgcgagtt cgacaccgtg gacctgagcg
     2881 ctgtggatgt gcaccccaac gtgtccatcg gcgtgttcat cgagcagccc accccctttc
     2941 tgccccggtt cctggacatc ctgctgaccc tggactaccc caaagaggcc ctgaagctgt
     3001 tcatccacaa caaagaggtg taccacgaga aggacatcaa ggtgttcttc gacaaggcca
     3061 agcacgagat caagaccatc aagatcgtgg gccccgagga aaatctgagc caggccgagg
     3121 ccagaaacat gggcatggat ttctgcagac aggacgagaa gtgcgactac tacttcagcg
     3181 tggacgccga cgtggtgctg accaaccccc ggaccctgaa gatcctgatc gagcagaacc
     3241 ggaagatcat tgcccccctg gtcaccagac acggcaagct gtggagcaac ttctggggag
     3301 ccctgagccc tgacggctac tacgccagat ccgaggacta cgtggacatc gtgcagggca
     3361 acagagtggg cgtgtggaac gtgccctaca tggccaacgt gtacctgatc aagggcaaga
     3421 cactgcggag cgagatgaac gagagaaact acttcgtgcg ggacaagctg gaccccgaca
     3481 tggccctgtg ccggaacgcc cgggagatgg gcgtgtttat gtacatcagc aaccggcacg
     3541 agttcggcag actgctgtcc accgccaact acaacaccag ccactacaac aacgacctgt
     3601 ggcagatctt cgagaacccc gtggactgga aagagaagta catcaaccgg gactactcca
     3661 agatcttcac cgagaacatc gtggagcagc cttgccccga cgtcttttgg ttccccatct
     3721 tcagcgagaa ggcctgcgac gaactggtgg aggaaatgga acattacggc aagtggagcg
     3781 gcggcaagca ccacgacagc cggatctccg gcggctacga gaacgtgccc accgacgaca
     3841 tccacatgaa acaggtggac ctggaaaacg tgtggctgca cttcatccgg gagtttatcg
     3901 cccctgtgac cctgaaagtg ttcgccggct actacaccaa aggcttcgcc ctgctgaatt
     3961 tcgtggtcaa gtacagcccc gagcggcagc gctctctgag gccccaccac gacgccagca
     4021 ccttcaccat caatatcgcc ctgaacaacg tgggcgagga cttccagggc ggagggtgca
     4081 agttcctgcg gtacaactgc agcatcgaga gcccccggaa gggctggtcc ttcatgcacc
     4141 ccggcagact gacacatctg cacgagggcc tgcccgtgaa gaacggcacc cggtacattg
     4201 ccgtgagctt catcgaccct ggcgcgtcta gaatggacga gctgtacaag ggctccggag
     4261 agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc cccatgaaaa
     4321 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4381 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4441 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4501 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4561 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4621 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     4681 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     4741 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     4801 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     4861 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     4921 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     4981 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5041 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5101 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5161 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5221 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5281 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg
     5341 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     5401 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     5461 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     5521 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     5581 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     5641 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     5701 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     5761 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     5821 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagatggtga gcaagggcga
     5881 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5941 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6001 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6061 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6121 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6181 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6241 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6301 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6361 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6421 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6481 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6541 cgccgccggg atcactctcg gcatggacga gctgtacaag gatatctccg gaggtatggt
     6601 cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca acctggacca
     6661 agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc
     6721 gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat
     6781 cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt
     6841 gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac tggtaatcga
     6901 cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca tcgccgtgtt
     6961 cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa ttatcgacga
     7021 gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg gagtgaccgg
     7081 ctggcggctg tgcgaacgca ttctggcgta aatcgatagt ggtccaggct ctagttttga
     7141 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     7201 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7261 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     7321 gatcaaggtc aggaacagat ggaacagggt cgaccctaga gaaccatcag atgtttccag
     7381 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     7441 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     7501 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     7561 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     7621 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     7681 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg
     7741 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     7801 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7861 gcgcagccat gacccagtca cgtagcgata gcggagtgta gatccggctg tggaatgtgt
     7921 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7981 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8041 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8161 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8221 tttttggagg cctaggcttt tgcaaaaagc ttactggctt aactatgcgg catcagagca
     8281 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     8341 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8941 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9601 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9901 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10261 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10321 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattag cttggccatt
    10381 gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc
    10441 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
    10501 tcatagccca tatatggagt
//
