LOCUS       sequence-271658-        9585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of gRNA against human PLOD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Sato K, Parag-Sharma K, Terajima M, Musicant AM, Murphy RM, Ramsey 
            MR, Hibi H, Yamauchi M, Amelio AL
  TITLE     Lysyl hydroxylase 2-induced collagen cross-link switching promotes 
            metastasis in head and neck squamous cell carcinomas.
  JOURNAL   Neoplasia. 2021 Jun 6;23(6):594-606. doi: 10.1016/j.neo.2021.05.014.
  PUBMED    34107376
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2021.05"; journalName: "Neoplasia"; date: "2021-06-6-
            6"; volume: "23"; issue: "6"; pages: "594-606"
COMMENT     Sequence Label: pSico_U6-PLOD2 sgRNA3
FEATURES             Location/Qualifiers
     source          1..9585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..581
                     /label=chicken beta-actin promoter
     intron          582..1598
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1522..1544
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1606..1625
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1663..1683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1687..1707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1711..1731
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1747..2442
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             2443..2496
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2940..2959)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3194..3227)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3283..3871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3336..3356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3754..3765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3874..3890)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3875..3891)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4400..4580
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4642..5230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4722..4741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5401..6192,6193..6261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6024..6043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6262..6366)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6441..6460)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6632..7011
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7013..7211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7166..7186
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7229..7409
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7456..7581
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8078..8311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8496..8540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8838..8955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(9208..9241)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9245..9265
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      481 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      541 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      601 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      661 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
      721 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
      781 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      841 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
      901 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
      961 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1021 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1081 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1141 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1201 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1261 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1321 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1381 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1441 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1501 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1561 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1621 ttgtgctgtc tcatcatttt ggcaaagaat tccgccacca tgccaaaaaa gaagagaaag
     1681 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt acacgtgtca
     1741 acgcgtatgg tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1801 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1861 acccagacca tgagaatcaa ggcggtcgag ggcggccctc tccccttcgc cttcgacatc
     1921 ctggctacca gcttcatgta cggcagcaaa accttcatca accacaccca gggcatcccc
     1981 gacttcttta agcagtcctt ccccgagggc ttcacatggg agagagtcac cacatacgaa
     2041 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     2101 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     2161 ctcggctggg aggcctccac cgagaccctg taccccgctg acggcggcct ggaaggcaga
     2221 gccgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt gaagaccaca
     2281 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggacaga
     2341 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     2401 gtggccagat actgcgacct ccctagcaaa ctggggcaca gagagggcag aggaagtctt
     2461 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga agaccttcaa catctcccag
     2521 caggatctag aattagtaga agtagcgaca gagaagatta caatgcttta tgaggataat
     2581 aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc ggcagtacat
     2641 attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat tggtagtgca
     2701 gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc ttattctgac
     2761 gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga gttgatttca
     2821 gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt caaaactacg
     2881 attgaagaac tcattccact caaatatacc cgaaattaat cgacatgata agatacattg
     2941 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3001 gtgatgctat tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3061 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcagag
     3121 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta gatcgaattc
     3181 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
     3241 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     3301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3421 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3481 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3541 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3601 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3661 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3721 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3781 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3841 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     3901 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     3961 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     4021 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4081 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     4141 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     4201 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     4261 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     4321 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     4381 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4501 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4561 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4621 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4681 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4741 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4801 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4861 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4921 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4981 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5041 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5101 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5161 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5221 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5281 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5701 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6001 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6361 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     6421 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     6481 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     6541 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     6601 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     6661 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     6721 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     6781 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     6841 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     6901 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     6961 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     7021 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     7081 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     7141 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     7201 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     7261 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7321 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7381 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7441 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7501 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7561 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7621 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7681 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7741 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7801 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7861 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7921 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     7981 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8041 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8101 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8161 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8221 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8281 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8341 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     8401 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     8461 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     8521 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     8581 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     8641 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     8701 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     8761 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
     8821 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8881 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8941 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg
     9001 ggcccgctct agagatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac
     9061 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
     9121 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
     9181 cattttacat gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta
     9241 taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
     9301 agtatccctt ggagaaccac cttgttggtc agccggcggc caatagccgt ttaagagcta
     9361 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     9421 caccgagtcg gtgctttttt tctcgagtac taggatccat taggcggccg cgtggataac
     9481 cgtattaccg ccatgcatcc cgggtgactg actgagcggc cgcactagtt attaatagta
     9541 atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
