LOCUS       sequence-27166-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.41
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..116
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    392..516
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(588..607)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(589..607)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(612..628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(612..628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttacgctacc atggagctcc aaataatgat tttattttga ctgatagtga cctgttcgtt
       61 gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtatagaa aagttggtcg
      121 acagaacatt tttccgtcaa ccggcaaata actactatta cagttatcct ggatacaatt
      181 attatcaagg aaagaagaga aagtagacta tttggacgat tattttgttc acgaaagaat
      241 attatcaata aaaaatgttc aatttaatgt ttttgtaata aaacaacgtg acacccttat
      301 gaacttaaag ttcatatttt attataaaaa gtagggggag gggggagaat tgaaatattt
      361 tgattatttc gcaaaccgac tagataaaat gacaagtttg tacaaaaaag ttgaacgaga
      421 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      481 ataatactgt aaaacacaac atatgcagtc actatgaacc aactacttag atggtattag
      541 tgacctgtag aattcgagct ctagagctgc agggcggccg cgatatcccc tatagtgagt
      601 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      661 gttacattgc acaagataaa aatatatcat cntgacnata aaactgtctg cttactaaca
      721 gtatacaggg gtgtatgagc ntatcacggg aacgtc
//
