LOCUS       sequence-271660-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an mScarlet-tagged gamma9 subunit for visualizing G 
            beta-gamma translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Copits BA, Gowrishankar R, O'Neill PR, Li JN, Girven KS, Yoo JJ, 
            Meshik X, Parker KE, Spangler SM, Elerding AJ, Brown BJ, Shirley SE,
            Ma KKL, Vasquez AM, Stander MC, Kalyanaraman V, Vogt SK, Samineni 
            VK, Patriarchi T, Tian L, Gautam N, Sunahara RK, Gereau RW 4th, 
            Bruchas MR
  TITLE     A photoswitchable GPCR-based opsin for presynaptic inhibition.
  JOURNAL   Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 
            10.1016/j.neuron.2021.04.026. Epub 2021 May 11.
  PUBMED    33979635
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.04.026"; journalName: "Neuron"; date: 
            "2021-06-2- 2"; volume: "109"; issue: "11"; pages: "1791-1809.e11"
COMMENT     Sequence Label: pcDNA3-mScarlet-gamma9
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             706..723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcat ggacgagctg tacaagggta ccatgcatca ccatcaccat
      721 cacgcccagg atctcagcga gaaggacctg ttgaagatgg aggtggagca gctgaagaaa
      781 gaagtgaaaa acacaagaat tccgatttcc aaagcgggaa aggaaatcaa ggagtacgtg
      841 gaggcccaag caggaaacga tccttttctc aaaggcatcc ctgaggacaa gaatcccttc
      901 aaggaaaaag gcggttgtat aataagctga gaattctgca gatatccagc acagtggcgg
      961 ccgctcgagt ctagagggcc cgtttaa
//
