LOCUS       sequence-271675-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugative vector with streamlined cloning capabilities. Contains 
            two cloning sites for modular insertion of genetic parts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    634..655
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        656..758
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             759..1418
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(825..844)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    1470..1490
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(1691..2542)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2529..3368)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3879..4850)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            6089..6176
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      6518..6912
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(7097..7912)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7097..7912)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7820..7839
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7913..8004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8030..8047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(8091..8454,1..428))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
       61 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
      121 gctggataac gccacggaat gatgtcgtcg tgcataacaa tggtgacttc tacagcgcgg
      181 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
      241 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
      301 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
      361 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      421 tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
      481 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
      541 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
      601 ccaccgctgc gttcggtcaa ggttctggac caggaattcg cggccgcttc tagagtgatc
      661 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      721 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      781 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      841 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      901 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      961 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1021 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1081 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1141 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1201 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1261 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1321 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1381 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     1441 gcccttaaac gcctggtgct acgcctgaat actagtagcg gccgctgcag gagcagaaga
     1501 gcatacatct ggaagcaaag ccaggaaagc ggcctatgga gctgtgcggc agcgctcagt
     1561 aggcaatttt tcaaaatatt gttaagcctt ttctgagcat ggtatttttc atggtattac
     1621 caattagcag gaaaataagc cattgaatat aaaagataaa aatgtcttgt ttacaataga
     1681 gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc gtacttgccc gccgcgaact
     1741 cggttaccgt ccagcccagc gcgaccagct ccggcaacgc ctcgcgcacc cgctggcggc
     1801 gcttgcgcat ggtcgaacca ctggcctctg acggccagac atagccgcac aaggtatcta
     1861 tggaagcctt gccggttttg ccggggtcga tccagccaca cagccgctgg tgcagcaggc
     1921 gggcggtttc gctgtccagc gcccgcacct cgtccatgct gatgcgcaca tgctggccgc
     1981 cacccatgac ggcctgcgcg atcaaggggt tcagggccac gtacaggcgc ccgtccgcct
     2041 cgtcgctggc gtactccgac agcagccgaa acccctgccg cttgcggcca ttctgggcga
     2101 tgatggatac cttccaaagg cgctcgatgc agtcctgtat gtgcttgagc gccccaccac
     2161 tatcgacctc tgccccgatt tcctttgcca gcgcccgata gctacctttg accaccatgg
     2221 catcagcggt gacggcctcc cacttgggtt ccaggaacag ccggagctgc cgtccgcctt
     2281 cggtcttggg ttccgggcca agcactaggc cattaggccc agccatggcc accagccctt
     2341 gcaggatgcg cagatcatca gcgcccagcg gctccgggcc gctgaactcg atccgcttgc
     2401 cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt gcgctcgccc cgcttgaggg
     2461 cacggaacag gccgggggcc agacagtgcg ccgggtcgtg ccggacgtgg ctgaggctgt
     2521 gcttgttctt aggcttcacc acggggcacc cccttgctct tgcgctgcct ctccagcacg
     2581 gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc gatcagcgaa cggtgcgcca
     2641 tagttggcct tgctcacacc gaagcggacg aagaaccggc gctggtcgtc gtccacaccc
     2701 cattcctcgg cctcggcgct ggtcatgctc gacaggtagg actgccagcg gatgttatcg
     2761 accagtaccg agctgccccg gctggcctgc tgctggtcgc ctgcgcccat catggccgcg
     2821 cccttgctgg catggtgcag gaacacgata gagcacccgg tatcggcggc gatggcctcc
     2881 atgcgaccga tgacctgggc catggggccg ctggcgtttt cttcctcgat gtggaaccgg
     2941 cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg cgcgcttgag gccgtcgaac
     3001 cactccgggg ccatgatgtt gggcaggctg ccgatcagcg gctggatcag caggccgtca
     3061 gccacggctt gccgttcctc ggcgctgagg tgcgccccaa gggcgtgcag gcggtgatga
     3121 atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg ggccggtggg cagttcgccc
     3181 acctccagca gatccggccc gcctgcaatc tgtgcggcca gttgcagggc cagcatggat
     3241 ttaccggcac caccgggcga caccagcgcc ccgaccgtac cggccaccat gttgggcaaa
     3301 acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca gaatattgat aggcttatgg
     3361 gtagccattg attgcctcct ttgcaggcag ttggtggtta ggcgctggcg gggtcactac
     3421 ccccgccctg cgccgctctg agttcttcca ggcactcgcg cagcgcctcg tattcgtcgt
     3481 cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt catgcgctcg gcatatcgcg
     3541 cttggcgtac agcgtcaggg ctggccagca ggtcgccggt ctgcttgtcc ttttggtctt
     3601 tcatatcagt caccgagaaa cttgccgggg ccgaaaggct tgtcttcgcg gaacaaggac
     3661 aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga ctgaaaagcg gccagcctcg
     3721 gccttgtttg acgtataacc aaagccaccg ggcaaccaat agcccttgtc acttttgatc
     3781 aggtagaccg accctgaagc gcttttttcg tattccataa aacccccttc tgtgcgtgag
     3841 tactcatagt ataacaggcg tgagtaccaa cgcaagcact acatgctgaa atctggcccg
     3901 cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg ccagctcggc ccgcgcaagc
     3961 tggacgctgg gcagacccat gaccttgctg acggtgcgct cgatgtaatc cgcttcgtgg
     4021 ccgggcttgc gctctgccag cgctgggctg gcctcggcca tggccttgcc gatttcctcg
     4081 gcactgcggc cccggctggc cagcttctgc gcggcgataa agtcgcactt gctgaggtca
     4141 tcaccgaagc gcttgaccag cccggccatc tcgctgcggt actcgtccag cgccgtgcgc
     4201 cggtggcggc taagctgccg ctcgggcagt tcgaggctgg ccagcctgcg ggccttctcc
     4261 tgctgccgct gggcctgctc gatctgctgg ccagcctgct gcaccagcgc cgggccagcg
     4321 gtggcggtct tgcccttgga ttcacgcagc agcacccacg gctgataacc ggcgcgggtg
     4381 gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc catagtggcg gctgtcggcg
     4441 ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg caatctgccc ccgaagttca
     4501 ccgcctgcgg cgtcggccac cttgacccat gcctgatagt tcttcgggct ggtttccact
     4561 accagggcag gctcccggcc ctcggctttc atgtcatcca ggtcaaactc gctgaggtcg
     4621 tccaccagca ccagaccatg ccgctcctgc tcggcgggcc tgatatacac gtcattgccc
     4681 tgggcattca tccgcttgag ccatggcgtg ttctggagca cttcggcggc tgaccattcc
     4741 cggttcatca tctggccggt ggtggcgtcc ctgacgccga tatcgaagcg ctcacagccc
     4801 atggccttga gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat cgggccacca
     4861 agcgcagcca gatcgagccg tcctcggttg tcagtggcgt caggtcgagc aagagcaacg
     4921 atgcgatcag cagcaccacc gtaggcatca tggaagccag catcacggtt agccatagct
     4981 tccagtgcca cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc acctcggcgg
     5041 ctacctcccg caactctttg gccagctcca cccatgccgc ccctgtctgg cgctgggctt
     5101 tcagccactc cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg acctgccggg
     5161 cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc gatctgctcc gctaactcgt
     5221 tgatgcctct ggatttcttc actctgtcga ttgcgttcat ggtctattgc ctcccggtat
     5281 tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt tcagggccac gtctgcccgg
     5341 tcggtgcgga tgccccggcc ttccatctcc accacgttcg gccccaggtg aacaccgggc
     5401 aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc gtggccagcc
     5461 cgctctaatg cccggttggc atggtcggcc catgcctcgc gggtctgctc aagccatgcc
     5521 ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt gccgttgtac
     5581 cgcttgaacc actgagcggc gggccgctcg atgccgtcat tgatccgctc ggagatcatc
     5641 aggtggcagt gcgggttctc gccgccaccg gcatggatgg ccagcgtata cggcaggcgc
     5701 tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg gtcgagggtc
     5761 agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc gcgttcatac
     5821 aggtcggcag catcccagta gtcggcgggc cgctcgacga actccggcat gtgcccggat
     5881 tcggcgtgca agacttcatc catgtcgcgg gcatacttgc cttcgcgctg gatgtagtcg
     5941 gccttggccc tggccgattg gccgcccgac ctgctgccgg ttttcgccgt aaggtgataa
     6001 atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc catgcaatgg ccctcggaga
     6061 gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga agagaaaccg gtaagtgcgc
     6121 cctcccctac aaagtagggt cgggattgcc gccgctgtgc ctccatgata gcctacgaga
     6181 cagcacatta acaatggggt gtcaagatgg ttaaggggag caacaaggcg gcggatcggc
     6241 tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga aattcagcgg gtgcgggcaa
     6301 gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa ggtgctggtg ggggccatga
     6361 ttttggccaa ggtgaacagc agcgagtggc cggaggatcg gctcatggcg gcaatggatg
     6421 cgtaccttga acgcgaccac gaccgcgcct tgttcggtct gccgccacgc cagaaggatg
     6481 agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg cccccaccct
     6541 tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt tctgcggggt
     6601 ttggtgtggg gtttagcggg ctttgcccgc ctttccccct gccgcgcagc ggtggggcgg
     6661 tgtgtagcct agcgcagcga atagaccagc tatccggcct ctggccgggc atattgggca
     6721 agggcagcag cgccccacaa gggcgctgat aaccgcgcct agtggattat tcttagataa
     6781 tcatggatgg atttttccaa caccccgcca gcccccgccc ctgctgggtt tgcaggtttg
     6841 ggggcgtgac agttattgca ggggttcgtg acagttattg caggggggcg tgacagttat
     6901 tgcaggggtt cgtgacagtt agtacgggag tgacgggggt accgagtcga tgtcgactaa
     6961 cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg gcacttttcg
     7021 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7081 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     7141 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     7201 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     7261 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     7321 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     7381 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     7441 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg gtcgctgtta aaaggacaat
     7501 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     7561 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     7621 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     7681 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     7741 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     7801 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     7861 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc atactcttcc
     7921 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7981 aatgtattta gaaaaataaa caaataggca tgcagcgctc ttccgcttcc tcgctcactg
     8041 actcgctacg ctcggtcgtt cgactctcga gagcggccga agcttagaca ttatttgccg
     8101 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
     8161 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     8221 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     8281 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     8341 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     8401 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggca
//
