LOCUS       sequence-271677-       10101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1265
FEATURES             Location/Qualifiers
     source          1..10101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(115..183,184..975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(333..352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1146..1734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1635..1654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1888..1905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2022..2043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2058..2063,2064..2081,2082..2088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2096..2112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2101..2123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2120..2136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2120..2136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2155..2175
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2157..2175
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2203..2429
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2430..2610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2657..2782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3275..3508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3693..3737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4004..4120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4154..4178
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4309..5487
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4540..5478
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5435..5455
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5504..5522
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5760..5777)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5987..6006
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      6162..6171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6174..6662
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(6668..6692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6723..7311
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6776..6796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7194..7205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7362..7861
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7397..7420)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7794..7813
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7875..8678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7938..7957)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8548..8567
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8822..9055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9127..9248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9164..9183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9218..9237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9288..9423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9444..9462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9472..9489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9472..9488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9481..9503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9630..10085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9717..9736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9927..9948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
       61 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      121 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      181 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      241 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      301 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      361 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      421 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      481 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      541 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      601 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      661 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      721 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      781 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      841 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      901 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      961 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1801 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1861 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1921 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1981 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2041 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2101 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2161 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     2221 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     2281 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     2341 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     2401 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     2461 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2521 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2581 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     2641 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2701 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2761 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2821 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2881 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2941 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3001 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3061 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3121 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3181 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3241 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3301 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3361 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3421 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3481 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3541 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3601 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3661 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3721 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3781 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3841 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3901 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     3961 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
     4021 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     4081 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgtccagt gtggtggaat
     4141 tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc accatgggaa ccgacattga
     4201 ttattgacta ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg
     4261 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     4321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4381 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4441 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4501 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     4561 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     4621 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     4681 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     4741 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     4801 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     4861 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     4921 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     4981 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     5041 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     5101 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     5161 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     5221 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     5281 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     5341 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     5401 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     5461 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata
     5521 gggagaccca agctggctag gtaagcttgg taccgagctc ggatccgttg tgagatgcgg
     5581 gagagggtcg accccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     5641 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5701 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5761 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5821 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5881 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5941 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     6001 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     6061 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     6121 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgccaccatg ggtatgaacc
     6181 cagccatcag cgtcgctctc ctgctctcag tcttgcaggt gtcccgaggg cagaaggtga
     6241 ccagcctgac agcctgcctg gtgaaccaaa accttcgcct ggactgccgc catgagaata
     6301 acaccaagga taactccatc cagcatgagt tcagcctgac ccgagagaag aggaagcacg
     6361 tgctctcagg cacccttggg atacccgagc acacgtaccg ctcccgcgtc accctctcca
     6421 accagcccta tatcaaggtc cttaccctag ccaacttcac caccaaggat gagggcgact
     6481 acttttgtga gcttcgcgtc tcgggcgcga atcccatgag ctccaataaa agtatcagtg
     6541 tgtatagaga caagctggtc aagtgtggcg gcataagcct gctggttcag aacacatcct
     6601 ggatgctgct gctgctgctt tccctctccc tcctccaagc cctggacttc atttctctgt
     6661 gatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     6721 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6781 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6841 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6901 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6961 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7021 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7081 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7141 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7201 tcaatccagc ggaccttcct tcccgcggcc tgctgcccgc tctgcggcct cttccgcgtc
     7261 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggacctg
     7321 caggattctg cagatatccg gactagtgat ctaattctac cgggtagggg aggcgctttt
     7381 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     7441 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     7501 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     7561 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     7621 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     7681 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     7741 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     7801 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     7861 gacctgcagc caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg
     7921 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7981 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     8041 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     8101 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     8161 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     8221 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     8281 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     8341 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     8401 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     8461 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8521 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8581 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8641 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac gcgtcgcccg ccccacgacc
     8701 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
     8761 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8821 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     8881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     9061 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9121 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9241 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     9301 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9361 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9421 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9481 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9541 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9601 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     9661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9841 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9961 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    10021 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    10081 aatttaggtg gcacttttcg g
//
