LOCUS       sequence-271678-        7624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha 
            fused to IgG1 Fc (V242K mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, 
            Procko E
  TITLE     Engineered receptors for human cytomegalovirus that are orthogonal 
            to normal human biology.
  JOURNAL   PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 
            10.1371/journal.ppat.1008647. eCollection 2020 Jun.
  PUBMED    32559251
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-06-19"; volume: "16(6):e1008647. doi"; pages: 
            " 10.1371/journal.ppat.1008647. eCollection 2020 Jun"
COMMENT     Sequence Label: pcDNA3-sPDGFRalpha-Fc-V242K
FEATURES             Location/Qualifiers
     source          1..7624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2981..2998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2987..3211
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3257..3685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3344..3363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3554..3575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3694..3714)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3699..4028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3879..4014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3941..3960
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4095..4889
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4149..4168)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4759..4778
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5063..5184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5100..5119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5154..5173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5233..5249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5233..5249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5246..5268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5257..5273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5281..5287,5288..5305,5306..5311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5326..5347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5464..5481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5635..6223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5715..5734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6394..7185,7186..7254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7017..7036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7255..7359)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7434..7453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgcca ccatggggac ttcccatccg gcgttcctgg tcttaggctg tcttctcaca
      721 gggctgagcc taatcctctg ccagctttca ttaccctcta tccttccaaa tgaaaatgaa
      781 aaggttgtgc agctgaattc atccttttct ctgagatgct ttggggagag tgaagtgagc
      841 tggcagtacc ccatgtctga agaagagagc tccgatgtgg aaatcagaaa tgaagaaaac
      901 aacagcggcc tttttgtgac ggtcttggaa gtgagcagtg cctcggcggc ccacacaggg
      961 ttgtacactt gctattacaa ccacactcag acagaagaga atgagcttga aggcaggcac
     1021 atttacatct atgtgccaga cccagatgta gcctttgtac ctctaggaat gacggattat
     1081 ttagtcatcg tggaggatga tgattctgcc attatacctt gtcgcacaac tgatcccgag
     1141 actcctgtaa ccttacacaa cagtgagggg gtggtacctg cctcctatga cagcagacag
     1201 ggctttaatg ggaccttcac tgtagggccc tatatctgtg aggccaccgt caaaggaaag
     1261 aagttccaga ccatcccatt taatgtttat gctttaaaag caacatcaga gctggatcta
     1321 gaaatggaag ctcttaaaac cgtgtataag tcaggggaaa cgattgtggt cacctgtgct
     1381 gtttttaaca atgagaaggt tgaccttcaa tggacttacc ctggagaagt gaaaggcaaa
     1441 ggcatcacaa tgctggaaga aatcaaagtc ccatccatca aattggtgta cactttgacg
     1501 gtccccgagg ccacggtgaa agacagtgga gattacgaat gtgctgcccg ccaggctacc
     1561 agggaggtca aagaaatgaa gaaagtcact atttctgtcc atgagaaagg tttcattgaa
     1621 atcaaaccca ccttcagcca gttggaagct gtcaacctgc atgaagtcaa acattttgtt
     1681 gtagaggtgc gggcctaccc acctcccagg atatcctggc tgaaaaacaa tctgactctg
     1741 attgaaaatc tcactgagat caccactgat gtggaaaaga ttcaggaaat aaggtatcga
     1801 agcaaattaa agctgatccg tgctaaggaa gaagacagtg gccattatac tattgtagct
     1861 caaaatgaag atgctgtgaa gagctatact tttgaactgt taactcaagt tccttcatcc
     1921 attctggact tggtcgatga tcaccatggc tcaactgggg gacagacggt gaggtgcaca
     1981 gctgaaggca cgccgcttcc tgatattgag tggatgatat gcaaagatat taagaaatgt
     2041 aataatgaaa cttcctggac tattttggcc aacaatgtct caaacatcat cacggagatc
     2101 cactcccgag acaggagtac cgtggagggc cgtgtgactt tcgccaaagt ggaggagacc
     2161 atcgccgtgc gatgcctggc taagaatctc cttggagctg agaaccgaga gctgaagctg
     2221 gtggctccca ccctgcgttc tgaaggtgga ggatccgaca aaactcacac atgcccaccg
     2281 tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag
     2341 gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac
     2401 gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag
     2461 acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc
     2521 ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc
     2581 ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg
     2641 tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct gacctgcctg
     2701 gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag
     2761 aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc
     2821 aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg
     2881 cacgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga
     2941 taactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3001 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3061 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3121 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3181 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3241 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3481 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3541 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3601 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3661 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3721 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3781 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3841 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3901 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3961 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4021 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4081 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4141 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4201 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4261 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4321 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4381 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4441 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4501 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4561 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4621 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4681 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4741 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4801 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4861 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4921 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4981 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5041 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5161 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5221 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5281 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5341 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5401 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5461 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5521 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5581 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5641 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5701 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5761 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5821 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5881 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5941 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6001 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6061 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7381 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     7441 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     7501 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7561 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     7621 cgtt
//
