LOCUS       sequence-271683-       12674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian fluorescent reporter plasmid for PDGFRA signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, 
            Procko E
  TITLE     Engineered receptors for human cytomegalovirus that are orthogonal 
            to normal human biology.
  JOURNAL   PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 
            10.1371/journal.ppat.1008647. eCollection 2020 Jun.
  PUBMED    32559251
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-06-19"; volume: "16(6):e1008647. doi"; pages: 
            " 10.1371/journal.ppat.1008647. eCollection 2020 Jun"
COMMENT     Sequence Label: pCEP4-PDGFRA-reporter
FEATURES             Location/Qualifiers
     source          1..12674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(709..727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        795..899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(900..968,969..1760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1118..1137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1931..2519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2420..2439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2959..3007
                     /note="synthetic polyadenylation signal"
     misc_feature    3021..3112
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3061..3080
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3291..3322
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(3363..3365,3366..3368,3369..4079)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3363..3365,3366..3368,3369..4079)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3408..3429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3669..3688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4016..4037
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4266..4387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4751..4896
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             4939..5976
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(5974..5993)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6018..6066
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        6720..7099
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7100..7303
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7254..7274
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7300..7324
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7406..7435
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    10683..10817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10960..10982)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt ttttggggtc
       61 tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct cctggtcttc
      121 cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc cctcttcttt
      181 gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct cctaggccat
      241 ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga gatcaataga
      301 catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc ccctccaaca
      361 gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc
      421 ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga aaactcccgc
      481 tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc
      541 tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc ttcaaggtca
      601 ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag
      661 cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac
      721 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1081 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1141 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1201 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1441 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1501 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1561 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1621 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1681 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1741 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2041 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     2101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct
     2581 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     2641 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgca aggatcttgc
     2701 cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt
     2761 ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg
     2821 gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa
     2881 gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg
     2941 aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     3001 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     3061 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctggcctaac
     3121 tggccggtac ctgagctcac tagcaggatg tccatattag gacatctagg atgtccatat
     3181 taggacatct aggatgtcca tattaggaca tctaggatgt ccatattagg acatctagga
     3241 tgtccatatt aggacatcta gatctggcct cggcggccaa gcttagacac tagagggtat
     3301 ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc caccggcgcg
     3361 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3421 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3481 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3541 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3601 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3661 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3721 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3781 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3841 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3901 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3961 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4021 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     4081 gtcatggttt tcccccggaa gtcgaagagc aggacgacgg gacgctgcca atgtcatgcg
     4141 cacaagagtc tggtatggat cgccacccgg ctgcgtgtgc cagcgcgaga atcaatgtat
     4201 aatgaggcgc gccctaaggc cgcgactcta gagtcggggc ggccggccgc ttcgagcaga
     4261 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4321 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4381 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4441 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagc gaacgccagc
     4501 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     4561 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     4621 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     4681 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     4741 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     4801 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     4861 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     4921 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     4981 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     5041 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     5101 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     5161 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     5221 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     5281 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     5341 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     5401 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     5461 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     5521 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     5581 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     5641 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     5701 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     5761 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     5821 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     5881 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     5941 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     6001 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     6061 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     6121 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     6181 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     6241 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     6301 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     6361 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     6421 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     6481 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     6541 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     6601 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     6661 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     6721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6961 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7021 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     7081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     7141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     7201 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     7261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     7321 gatctctaga agctgggtac cagctgctag cgccaccatg aagaccatca tcgccctgag
     7381 ctacatcttc tgcctggtgt tcgccgagca gaaattgatc agcgaggagg acctgggcag
     7441 ccctggaggc gcctctggcc agctttcatt accctctatc cttccaaatg aaaatgaaaa
     7501 ggttgtgcag ctgaattcat ccttttctct gagatgcttt ggggagagtg aagtgagctg
     7561 gcagtacccc atgtctgaag aagagagctc cgatgtggaa atcagaaatg aagaaaacaa
     7621 cagcggcctt tttgtgacgg tcttggaagt gagcagtgcc tcggcggccc acacagggtt
     7681 gtacacttgc tattacaacc acactcagac agaagagaat gagcttgaag gcaggcacat
     7741 ttacatctat gtgccagacc cagatgtagc ctttgtacct ctaggaatga cggattattt
     7801 agtcatcgtg gaggatgatg attctgccat tataccttgt cgcacaactg atcccgagac
     7861 tcctgtaacc ttacacaaca gtgagggggt ggtacctgcc tcctatgaca gcagacaggg
     7921 ctttaatggg accttcactg tagggcccta tatctgtgag gccaccgtca aaggaaagaa
     7981 gttccagacc atcccattta atgtttatgc tttaaaagca acatcagagc tggatctaga
     8041 aatggaagct cttaaaaccg tgtataagtc aggggaaacg attgtggtca cctgtgctgt
     8101 ttttaacaat gaggtggttg accttcaatg gacttaccct ggagaagtga aaggcaaagg
     8161 catcacaatg ctggaagaaa tcaaagtccc atccatcaaa ttggtgtaca ctttgacggt
     8221 ccccgaggcc acggtgaaag acagtggaga ttacgaatgt gctgcccgcc aggctaccag
     8281 ggaggtcaaa gaaatgaaga aagtcactat ttctgtccat gagaaaggtt tcattgaaat
     8341 caaacccacc ttcagccagt tggaagctgt caacctgcat gaagtcaaac attttgttgt
     8401 agaggtgcgg gcctacccac ctcccaggat atcctggctg aaaaacaatc tgactctgat
     8461 tgaaaatctc actgagatca ccactgatgt ggaaaagatt caggaaataa ggtatcgaag
     8521 caaattaaag ctgatccgtg ctaaggaaga agacagtggc cattatacta ttgtagctca
     8581 aaatgaagat gctgtgaaga gctatacttt tgaactgtta actcaagttc cttcatccat
     8641 tctggacttg gtcgatgatc accatggctc aactggggga cagaccgtac ggtgcacagc
     8701 tgaaggcacg ccgcttcctg atattgagtg gatgatatgc aaagatatta agaaatgtaa
     8761 taatgaaact tcctggacta ttttggccaa caatgtctca aacatcatca cggagatcca
     8821 ctcccgagac aggagtaccg tggagggccg tgtgactttc gccaaagtgg aggagaccat
     8881 cgccgtgcga tgcctggcta agaatctcct tggagctgag aaccgagagc tgaagctggt
     8941 ggctcccacc ctgcgttctg aactcacggt ggctgctgca gtcctggtgc tgttggtgat
     9001 tgtgatcatc tcacttattg tcctggttgt catttggaaa cagaaaccga ggtatgaaat
     9061 tcgctggagg gtcattgaat caatcagccc ggatggacat gaatatattt atgtggaccc
     9121 gatgcagctg ccttatgact caagatggga gtttccaaga gatggactag tgcttggtcg
     9181 ggtcttgggg tctggagcgt ttgggaaggt ggttgaagga acagcctatg gattaagccg
     9241 gtcccaacct gtcatgaaag ttgcagtgaa gatgctaaaa cccacggcca gatccagtga
     9301 aaaacaagct ctcatgtctg aactgaagat aatgactcac ctggggccac atttgaacat
     9361 tgtaaacttg ctgggagcct gcaccaagtc aggccccatt tacatcatca cagagtattg
     9421 cttctatgga gatttggtca actatttgca taagaatagg gatagcttcc tgagccacca
     9481 cccagagaag ccaaagaaag agctggatat ctttggattg aaccctgctg atgaaagcac
     9541 acggagctat gttattttat cttttgaaaa caatggtgac tacatggaca tgaagcaggc
     9601 tgatactaca cagtatgtcc ccatgctaga aaggaaagag gtttctaaat attccgacat
     9661 ccagagatca ctctatgatc gtccagcctc atataagaag aaatctatgt tagactcaga
     9721 agtcaaaaac ctcctttcag atgataactc agaaggcctt actttattgg atttgttgag
     9781 cttcacctat caagttgccc gaggaatgga gtttttggct tcaaaaaatt gtgtccaccg
     9841 tgatctggct gctcgcaacg tcctcctggc acaaggaaaa attgtgaaga tctgtgactt
     9901 tggcctggcc agagacatca tgcatgattc gaactatgtg tcgaaaggca gtacctttct
     9961 gcccgtgaag tggatggctc ctgagagcat ctttgacaac ctctacacca cactgagtga
    10021 tgtctggtct tatggcattc tgctctggga gatcttttcc cttggtggca ccccttaccc
    10081 cggcatgatg gtggattcta ctttctacaa taagatcaag agtgggtacc ggatggccaa
    10141 gcctgaccac gctaccagtg aagtctacga gatcatggtg aaatgctgga acagtgagcc
    10201 ggagaagaga ccctcctttt accacctgag tgagattgtg gagaatctgc tgcctggaca
    10261 atataaaaag agttatgaaa aaattcacct ggacttcctg aagagtgacc atcctgctgt
    10321 ggcacgcatg cgtgtggact cagacaatgc atacattggt gtcacctaca aaaacgagga
    10381 agacaagctg aaggactggg agggtggtct ggatgagcag agactgagcg ctgacagtgg
    10441 ctacatcatt cctctgcctg acattgaccc tgtccctgag gaggaggacc tgggcaagag
    10501 gaacagacac agctcgcaga cctctgaaga gagtgccatt gagacgggtt ccagcagttc
    10561 caccttcatc aagagagagg acgagaccat tgaagacatc gacatgatgg acgacatcgg
    10621 catagactct tcagacctgg tggaagacag cttcctgtaa tgactcgagg ccggcaaggc
    10681 cggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
    10741 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
    10801 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
    10861 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
    10921 atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10981 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11041 gtcagcgggt gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatc
    11101 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
    11161 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
    11221 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
    11281 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
    11341 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
    11401 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
    11461 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
    11521 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
    11581 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
    11641 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
    11701 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
    11761 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
    11821 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
    11881 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
    11941 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
    12001 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
    12061 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
    12121 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
    12181 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
    12241 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
    12301 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
    12361 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
    12421 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
    12481 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
    12541 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
    12601 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
    12661 atctggatag cata
//
