LOCUS       sequence-27169-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.108
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..114
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    490..614
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(686..705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(687..705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(710..726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttacgctcca tggagctcca ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtatagaaaa gttgtattta
      121 aatttttaaa aaatttttcg agattagaat tcaaaaaaac gcaaaattta cccaaaaaag
      181 taagaaaacg gaaaagaaaa ttgggacgag atcgaaattg caacttccgc gttcgcgtcg
      241 agttgttcgt ttcgagaccc gtagatctga aacttccatg cgcctttaat aatttctaag
      301 tttctgaact atttccagat ttattttacc tattttctag tttttttttt caaatcaaaa
      361 tatgtttaat caatgcgggg ttttataatt aaataatatg tttaacttgt tatagatttt
      421 atagatatta ttttttaaac attttaattt gtttcacgat gatatttcag taattgtagg
      481 tttgcgatga caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc
      541 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
      601 atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct
      661 agagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
      721 ttcctggcag ctctggcccg tgtctcaaat ctctgagtta cttgc
//
