LOCUS       sequence-271703-        9615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutively active 
            dominant-negative HSF1 fused to DHFR destabilized domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Moore CL, Dewal MB, Nekongo EE, Santiago S, Lu NB, Levine SS, 
            Shoulders MD
  TITLE     Transportable, Chemical Genetic Methodology for the Small 
            Molecule-Mediated Inhibition of Heat Shock Factor 1.
  JOURNAL   ACS Chem Biol. 2016 Jan 15;11(1):200-10. doi: 
            10.1021/acschembio.5b00740. Epub 2015 Nov 19.
  PUBMED    26502114
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00740"; journalName: "ACS Chem Biol"; date: 
            "2016-01-15- 15"; volume: "11"; issue: "1"; pages: "200-10"
COMMENT     Sequence Label: pLenti CMV Puro DHFR.dn-cHSF1
FEATURES             Location/Qualifiers
     source          1..9615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        143..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     647..671
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    755..779
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             806..1282
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    complement(2422..2446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2477..3065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2530..2550)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2948..2959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3079..3578
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3114..3137)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3511..3530
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3599..4198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3599..3618)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4095..4115
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4336..4569
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4641..4762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4678..4697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4732..4751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4802..4937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4957..4976)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4958..4976)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4986..5003)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4986..5002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4995..5017)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5144..5599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5231..5250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5441..5462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5625..5729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5730..5798,5799..6590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5948..5967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6761..7349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7250..7269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7503..7520
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7637..7658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7673..7678,7679..7696,7697..7703)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7711..7727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7716..7738
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7735..7751
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7735..7751
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7770..7790
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7772..7790
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7818..8044
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8045..8225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8272..8397
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8890..9123
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9308..9352
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
       61 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
      121 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      661 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
      721 tctagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt
      781 taaaggaacc aattcagtcg acaccatgat cagtctgatt gcggcgttag cggtagatta
      841 cgttatcggc atggaaaacg ccatgccgtg gaacctgcct gccgatctcg cctggtttaa
      901 acgcaacacc ttaaataaac ccgtgattat gggccgccat acctgggaat caatcggtcg
      961 tccgttgcca ggacgcaaaa atattatcct cagcagtcaa ccgagtacgg acgatcgcgt
     1021 aacgtgggtg aagtcggtgg atgaagccat cgcggcgtgt ggtgacgtac cagaaatcat
     1081 ggtgattggc ggcggtcgcg ttattgaaca gttcttgcca aaagcgcaaa aactgtatct
     1141 gacgcatatc gacgcagaag tggaaggcga cacccatttc ccggattacg agccggatga
     1201 ctgggaatcg gtattcagcg aattccacga tgctgatgcg cagaactctc acagctattg
     1261 ctttgagatt ctggagcggc gagcaggaag tggaagtgga tccgccatgg atctgcccgt
     1321 gggccccggc gcggcggggc ccagcaacgt cccggccttc ctgaccaagc tgtggaccct
     1381 cgtgagcgac ccggacaccg acgcgctcat ctgctggagc ccgagcggga acagcttcca
     1441 cgtgttcgac cagggccagt ttgccaagga ggtgctgccc aagtacttca agcacaacaa
     1501 catggccagc ttcgtgcggc agctcaacat gtatggcttc cggaaagtgg tccacatcga
     1561 gcagggcggc ctggtcaagc cagagagaga cgacacggag ttccagcacc catgcttcct
     1621 gcgtggccag gagcagctcc ttgagaacat caagaggaaa gtgaccagtg tgtccaccct
     1681 gaagagtgaa gacataaaga tccgccagga cagcgtcacc aagctgctga cggacgtgca
     1741 gctgatgaag gggaagcagg agtgcatgga ctccaagctc ctggccatga agcatgagaa
     1801 tgaggctctg tggcgggagg tggccagcct tcggcagaag catgcccagc aacagaaagt
     1861 catcctgggg gtgaagagaa agatccccct gatgctgaac gacagtggct cagcacattc
     1921 catgcccaag tatagccggc agttctccct ggagcacgtc cacggctcgg gcccctactc
     1981 ggccccctcc ccagcctaca gcagctccag cctctacgcc cctgatgctg tggccagctc
     2041 tggacccatc atctccgaca tcaccgagct ggctcctgcc agccccatgg cctcccccgg
     2101 cgggagcata gacgagaggc ccctatccag cagccccctg gtgcgtgtca aggaggagcc
     2161 ccccagcccg cctcagagcc cccgggtaga ggaggcgagt cccgggcgcc catcttccgt
     2221 ggacaccctc ttgtccccga ccgccctcat tgactccatc ctgcgggaga gtgaacctgc
     2281 ccccgcctcc gtcacagccc tcacggacgc caggggccac acggacaccg agggccggcc
     2341 tccctccccc ccgcccacct ccacccctga aaagtgcctc agcgtagcct gcctgtaggc
     2401 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat atccagcaca
     2461 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2521 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2581 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2641 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2701 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2761 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2821 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     2881 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2941 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3001 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3061 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     3121 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     3181 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     3241 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     3301 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3361 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     3421 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3481 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3541 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3601 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3661 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3721 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3781 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3841 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3901 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3961 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4021 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4081 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4141 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     4201 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     4261 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4321 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     4381 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4561 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4621 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4681 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4741 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4861 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4921 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4981 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5041 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5101 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5161 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5221 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5281 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5341 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5401 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5461 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5521 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5581 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7381 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7441 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7501 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7561 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7621 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7681 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7741 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7801 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7861 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7921 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7981 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8041 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8221 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8281 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8341 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8401 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8461 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8521 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8581 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8641 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8701 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8761 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8821 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8881 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8941 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9001 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9061 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9121 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9181 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9241 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9301 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9361 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9421 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9481 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     9541 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     9601 tcgacggtat cggtt
//
