LOCUS       sequence-271706-        3175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_10
FEATURES             Location/Qualifiers
     source          1..3175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     77..93
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     77..93
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     98..117
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        98..116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    134..233
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(865..964)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(980..997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(980..996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(989..1011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1060..1146
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1238..1265
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1424..1441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1595..2183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1675..1694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2330..3139)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3053..3072
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3131..3150
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
       61 cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat
      121 atcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
      181 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtcggcgcg
      241 cccacccttc cccatctcat actattgggg ttcaggttcc aggagtagac cccatcagac
      301 ttgcgtgtgt gcatgcgtat attgcatagt gaaatatata cacacattta tgattcttca
      361 ccttgaggta attcaatgaa tagaaaccca gaatcgtgag ctctgtggtt tactaaagcc
      421 ccatggaaag ccctactgca gtagctatgc aaagttctgg ttaaacattg tcccagcgtt
      481 tgcactgctc cgtctgttgt ttttacgcta aagactcagc ccaggttaga ggcggtattg
      541 actttgtaat taaaccattt taccttcaga attttgtaat cactgcattt aaaaccatct
      601 gttgtagtga gaaggtaaac tatttttccc ctcaaagtac agaaaaaaaa aactacaggg
      661 cttttttttt ccttaaacgt cagagttctg attttagggc attgatttgc ttaacatatc
      721 agttaaaaaa aaaaaaggaa aaaatttatc tctgagagat ggaaaagaaa gactgtcata
      781 gaattcaaaa taaatggagg tgaaagtatc ttcttacctg ctgctgtttt tcaaaggtgt
      841 tttgggtgaa gggggcggcc gcggagcctg cttttttgta caaagttggc attataaaaa
      901 agcattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      961 tttggggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc
     1021 atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
     1081 tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1141 acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca
     1201 ggacgcccgc cataaactgc caggcatcaa actaagcaga aggccatcct gacggatggc
     1261 ctttttgcgt ttctacaaac tcttcctggc tagcggtacg cgtattaatt gcgttgcgct
     1321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2221 agtggaacga cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
     2281 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     2341 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     2401 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     2461 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     2521 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     2581 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     2641 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     2701 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     2761 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     2821 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     2881 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     2941 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     3001 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     3061 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     3121 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacag
//
