LOCUS       sequence-27172-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.128
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..115
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    390..513
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(585..604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(586..604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(609..625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttacgctcca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg
       61 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttggtcga
      121 cagaacattt ttccgtcaac cggcaaataa ctactattac agttatcctg gatacaatta
      181 ttatcaagga aagaagagaa agtagactat ttggacgatt attttgttca cgaaagaata
      241 ttatcaataa aaaatgttca atttaatgtt tttgtaataa aacaacgtga cacccttatg
      301 aacttaaagt tcatatttta ttataaaaag tagggggagg ggggagaatt gaaatatttt
      361 gattatttcg caaaccgact agataaaatc aagtttgtac aaaaaagttg aacgagaaac
      421 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      481 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
      541 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
      601 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      661 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      721 gtattc
//
