LOCUS       sequence-271726-        4076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid for expression of E. coli flagellin from 
            its natural promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Thomson NM, Pallen MJ
  TITLE     Restoration of wild-type motility to flagellin-knockout Escherichia 
            coli by varying promoter, copy number and induction strength in 
            plasmid-based expression of flagellin.
  JOURNAL   Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 
            10.1016/j.crbiot.2020.03.001.
  PUBMED    33381753
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Res 
            Biotechnol."; date: "2020-11"; volume: "2:45-52. doi"; pages: " 
            10.1016/j.crbiot.2020.03.001"
COMMENT     Sequence Label: pJ211_WT_hi
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2175..2763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2255..2274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2768..3577)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(3578..3669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3736..3755
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gctgtttttg aaaaaaattc taaaggttgt tttacgacag acgataacag ggttgacggc
       61 gattgagccg acgggtggaa acccaatacg taatcaacga cttgcaatat aggataacga
      121 accatggcac aagtcattaa taccaacagc ctctcgctga tcactcaaaa taatatcaac
      181 aagaaccagt ctgcgctgtc gagttctatc gagcgtctgt cttctggctt gcgtattaac
      241 agcgcgaagg atgacgcagc gggtcaggcg attgctaacc gtttcacctc taacattaaa
      301 ggcctgactc aggcggcccg taacgccaac gacggtatct ccgttgcgca gaccaccgaa
      361 ggcgcgctgt ccgaaatcaa caacaactta cagcgtgtgc gtgaactgac ggtacaggcc
      421 actaccggta ctaactctga gtctgatctg tcttctatcc aggacgaaat taaatcccgt
      481 ctggatgaaa ttgaccgcgt atctggtcag acccagttca acggcgtgaa cgtgctggca
      541 aaaaatggct ccatgaaaat ccaggttggc gcaaatgata accagactat cactatcgat
      601 ctgaagcaga ttgatgctaa aactcttggc cttgatggtt ttagcgttaa aaataacgat
      661 acagttacca ctagtgctcc agtaactgct tttggtgcta ccaccacaaa caatattaaa
      721 cttactggaa ttaccctttc tacggaagca gccactgata ctggcggaac taacccagct
      781 tcaattgagg gtgtttatac tgataatggt aatgattact atgcgaaaat caccggtggt
      841 gataacgatg ggaagtatta cgcagtaaca gttgctaatg atggtacagt gacaatggcg
      901 actggagcaa cggcaaatgc aactgtaact gatgcaaata ctactaaagc tacaactatc
      961 acttcaggcg gtacacctgt tcagattgat aatactgcag gttccgcaac tgccaacctt
     1021 ggtgctgtta gcttagtaaa actgcaggat tccaagggta atgataccga tacatatgcg
     1081 cttaaagata caaatggcaa tctttacgct gcggatgtga atgaaactac tggtgctgtt
     1141 tctgttaaaa ctattaccta tactgactct tccggtgccg ccagttctcc aaccgcggtc
     1201 aaactgggcg gagatgatgg caaaacagaa gtggtcgata ttgatggtaa aacatacgat
     1261 tctgccgatt taaatggcgg taatctgcaa acaggtttga ctgctggtgg tgaggctctg
     1321 actgctgttg caaatggtaa aaccacggat ccgctgaaag cgctggacga tgctatcgca
     1381 tctgtagaca aattccgttc ttccctcggt gcggtgcaaa accgtctgga ttccgcggtt
     1441 accaacctga acaacaccac taccaacctg tctgaagcgc agtcccgtat tcaggacgcc
     1501 gactatgcga ccgaagtgtc caatatgtcg aaagcgcaga tcatccagca ggccggtaac
     1561 tccgtgttgg caaaagctaa ccaggtaccg cagcaggttc tgtctctgct gcagggttaa
     1621 gaattctgat cacactggct acctttgggt ggcctttttt tatcgtcaaa agggcgacac
     1681 aaaaggtatt ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt
     1741 gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc
     1801 gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat
     1861 aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta
     1921 tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa
     1981 agtgacaggc gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa
     2041 aaacccgccg aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac
     2101 cgcccaggat gcctggcagt tccctactct cgccgctgcg ctcggtcgtt cggctgcggg
     2161 acctcagygc tagctttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2221 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2281 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2341 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2401 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2461 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2521 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2581 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2641 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2701 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2761 caagctttta gaaaaactca tcgagcatca aatgaaattg caatttattc atatcaggat
     2821 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     2881 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     2941 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     3001 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     3061 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3121 gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3181 gaatcgagtg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     3241 caggatattc ttctaatacc tggaacgctg tttttccggg gatcgcagtg gtgagtaacc
     3301 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagtggcata aattccgtca
     3361 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     3421 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
     3481 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     3541 atcgcggcct cgacgtttcc cgttgaatat ggctcatatt cttccttttt caatattatt
     3601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3661 ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc
     3721 catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg
     3781 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     3841 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     3901 gaaagactgg gcctttcgcc cgggctaatt agggggtgtc gcccttcgct gaagtcgacg
     3961 taaaacgaat accggggtta tcggtctgaa ttgcgcaaag tttacgttta attgtttttt
     4021 ttaatagcgg gaataagggg cagagaaaag agtatttcgg cgactaacaa aaaatg
//
