LOCUS       sequence-271754-        6861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM149
FEATURES             Location/Qualifiers
     source          1..6861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             87..503
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     433..455
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     471..487
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      538..547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(544..546,547..549,550..1260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(544..546,547..549,550..1260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(589..610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(850..869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1197..1218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1306..1326
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1339..1383
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2591..2611)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2596..2925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2776..2911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2933..3331
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3500..3969
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4099..4116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4270..4858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4350..4369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5018..5809,5810..5878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5641..5660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5879..5983)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6014..6483
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6548..6747
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
       61 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
      121 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
      181 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
      241 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
      301 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
      361 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
      421 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
      481 ccctcactcc ttctctaggc gccggccgga tcccagtgtg gtggtacgct accggtcgcc
      541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1261 tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt
     1321 cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     1381 agcggaggtg gaggtactag tctcgagatg gaagatagcg gcaaaacctt cagcagcgaa
     1441 gaagaagagg cgaattactg gaaggacctg gccatgacct acaagcagcg tgccgaaaat
     1501 acccaggaag aactgcgcga atttcaagag gggagccgcg aatatgaggc cgagctggaa
     1561 acacagctgc agcagatcga gacacgcaat cgcgatctgc tgagcgaaaa taaccgcctg
     1621 cgcatggaac tggaaacaat taaagagaaa ttcgaggtgc agcatagcga gggctatcgc
     1681 caaattagcg ccctggagga tgatctggcc cagaccaaag ccatcaaaga tcagctgcag
     1741 aaatacattc gcgagctggc ccaggcaaat gatgatctgg aacgcgccaa agccgccacc
     1801 attatgagtc tggaggactt tgagcagcgt ctgaaccagg caattgagcg caatgccttc
     1861 ctggaaagcg aattagatga gaaagagaat ctgttagaga gtgtgcagcg cctgaaagac
     1921 gaagcccgtg acctgcgcca ggagctggca gtgcagcaga agcaagaaaa gccgcgcacc
     1981 ccgatgccga gtagtgttga agcagaacgt accgataccg cagtgcaagc caccggcagc
     2041 gttccgagta caccgattgc ccatcgcggc ccgagtagca gtctgaatac cccgggcagc
     2101 tttcgtcgtg gtctggatga tagcaccggt ggcacaccgt taacaccggc cgcccgtatt
     2161 agcgccctga atattgtggg cgatctgctg cgcaaagtgg gcgcactgga gagtaaactg
     2221 gccagttgcc gcaacctggt gtatgatcag agcccgaatc gtacaggtgg tccggcaagc
     2281 ggccgcagta gtaaaaatcg tgatggcggc gaacgccgtc cgagtagtac cagcgttccg
     2341 ctgggcgaca aaggcctgga taccagctgc cgttggctga gcaaaagcac cacccgcagc
     2401 agtagcagtt gttaagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     2461 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     2521 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattcgcc agcacagtgg
     2581 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2641 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2761 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2821 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2881 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcatggccaa
     2941 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     3001 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     3061 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     3121 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     3181 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     3241 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     3301 gctgccctct ggttatgtgt gggagggcta agatcgataa aataaaagat tttatttagt
     3361 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     3421 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
     3481 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3541 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     3601 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     3661 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     3721 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     3781 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     3841 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     3901 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     3961 ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     4021 taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4081 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat
     4981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6001 tgccacctgc agcctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     6061 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     6121 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     6181 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     6241 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     6301 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     6361 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     6421 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     6481 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc
     6541 gggaggcaag ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg
     6601 attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg
     6661 gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc agggactttg
     6721 ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg accccgtcag
     6781 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     6841 tgctttcggt ttggaaccga a
//
