LOCUS       sequence-271755-        8629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of gRNAs targeting intron 4 of murine Qk and 
            intron 9 of murine Myb. Also constitutively expresses Puromycin 
            linked to TagBFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Oldrini B, Curiel-Garcia A, Marques C, Matia V, Uluckan O, 
            Grana-Castro O, Torres-Ruiz R, Rodriguez-Perales S, Huse JT, 
            Squatrito M
  TITLE     Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for 
            precision tumor modeling.
  JOURNAL   Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w.
  PUBMED    29654229
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-13"; volume: "9(1):1466. doi"; pages: " 
            10.1038/s41467-018-03731-w"
COMMENT     Sequence Label: pKLV-gRNA-Myb-Qk_1
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        715..1018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1020..1218
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1173..1193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1236..1416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1463..1588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2085..2318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2503..2547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2845..2962
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3045..3293
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3045..3065
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3216..3235
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3314..3389
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3486..3799
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     3718..3738
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        3823..3898
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3921..4420
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3956..3979)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4353..4372
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4460..5056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4460..4479)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4956..4976
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5075..5128
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5129..5830
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(6185..6202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6191..6415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6441..6461)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6441..6459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6480..6496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6480..6496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6493..6515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6504..6520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6528..6534,6535..6552,6553..6558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6573..6594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6711..6728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6882..7470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6962..6981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7641..8432,8433..8501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8264..8283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8502..8606)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtta
      661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      721 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      781 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      841 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      901 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      961 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1021 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1081 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1141 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1201 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     1261 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1321 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1381 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1441 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1501 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1561 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1621 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1681 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1741 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1801 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1861 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1921 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1981 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2041 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2101 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2161 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2221 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2281 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     2341 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     2401 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2461 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2521 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2581 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2641 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2701 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2761 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     2821 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2881 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2941 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     3001 agtaccgggc cctacgcgtt actcgagcca aggtcgggca ggaagagggc ctatttccca
     3061 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     3121 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3181 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     3241 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgaacttc
     3301 attaattcac accgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     3361 aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt
     3421 aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagat
     3481 ctagagatcc gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg
     3541 agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata
     3601 gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta
     3661 catgataggc ttggatttct ataagagata caaatactaa attattattt taaaaaacag
     3721 cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatgc
     3781 atgcgagaaa agccttgttt ggttccctca atctaactac aagttttaga gctagaaata
     3841 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     3901 tttttggatc caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     3961 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     4021 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     4081 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     4141 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     4201 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     4261 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     4321 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     4381 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
     4441 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4501 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4561 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4621 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4681 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4741 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4801 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4861 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4921 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4981 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5041 ccgcaagccc ggtgccggcg gcgggtccgg aggagagggc agaggaagtc tcctaacatg
     5101 cggtgacgtg gaggagaatc ctggcccaat gagcgagctg attaaggaga acatgcacat
     5161 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     5221 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     5281 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     5341 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     5401 gagagtcacc acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca
     5461 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     5521 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     5581 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     5641 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     5701 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     5761 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     5821 gcttaattga gcggccgcta ggtaccttta agaccaatga cttacaaggc agctgtagat
     5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaaagaagt
     5941 caagatctgc tttttgcctg tactgggtct ctctggttag accagagtct ctctggttag
     6001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagttta aacccgctga
     6181 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6241 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6301 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6361 ggggaggatt gggaagtcaa tagcaggcat gctggggatg cggtgggctc tatgggcggc
     6421 cgttaattaa gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     6481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6541 gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
