LOCUS       sequence-27177-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.34
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..175)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(476..571)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(608..627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(609..627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(632..648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(632..648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt tcatagtgac
       61 tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa agtgggataa
      181 ggtggaactt tctcaccata aatctcatcc cacccactta tgttctgttg ttttgtaata
      241 ttttaaactg gtgcaatgat ttcgtttcga ttttaagatc gatgttgtgc cttccccact
      301 cttatcattg cccagttttg ttttttcttt tcttttttgt tattttattg ctttttatgc
      361 agttgtgtta ttgtttcttt ctgtttactg ttcattgttt ttgttaattt tttattttcg
      421 attttaatga atccagataa aattagacaa cttggcaaaa tatcgttcga gtcttcaact
      481 ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag
      541 gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt aacgcggccg
      601 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
      661 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      721 aaactgtctg cttacataaa cagta
//
