LOCUS       sequence-271773-       11493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of untagged Casilio-ME1 
            effector PUFa-hTET1(CD) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 11493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT888_CMV/CAG_PUFa-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..11493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4769..4789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4817..4837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4928..4948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6005..6025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6029..6049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(8294..8318)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8320..8361
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8403..8991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8456..8476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8874..8885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9063..9296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9374..9495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9411..9430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9465..9484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9535..9670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9690..9709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9691..9709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9719..9736)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9719..9735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9728..9750)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9877..10332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9964..9983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10174..10195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10358..10462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10463..10531,10532..11323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10681..10700)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgatcgat ggcggaggcg gatctgatcc aaaaaagaag agaaaggtag atccaaaaaa
     4801 gaagagaaag gtagatccaa aaaagaagag aaaggtagga tctaccggat ctagaaacga
     4861 tggtggtggt ggaagcgggg gtgggggcag cggtggaggg ggaagcgggc gcgccgggat
     4921 cctccccccc aagaaaaaga ggaaggtatc tagaggccgc agccgccttt tggaagattt
     4981 tcgaaacaac cggtacccca atttacaact gcgggagatt gctggacata taatggaatt
     5041 ttcccaagac cagcatgggt ccagattcat tcagctgaaa ctggagcgtg ccacaccagc
     5101 tgagcgccag cttgtcttca atgaaatcct ccaggctgcc taccaactca tggtggatgt
     5161 gtttggtaat tacgtcattc agaagttctt tgaatttggc agtcttgaac agaagctggc
     5221 tttggcagaa cggattcgag gccacgtcct gtcattggca ctacagatgt atggcagccg
     5281 tgttatcgag aaagctcttg agtttattcc ttcagaccag cagaatgaga tggttcggga
     5341 actagatggc catgtcttga agtgtgtgaa agatcagaat ggcaatcacg tggttcagaa
     5401 atgcattgaa tgtgtacagc cccagtcttt gcaatttatc atcgacgcgt ttaagggaca
     5461 ggtatttgcc ttatccacac atccttatgg ctgccgagtg attcagagaa tcctggagca
     5521 ctgtctccct gaccagacac tccctatttt agaggagctt caccagcaca cagagcagct
     5581 ggtacaggat caatatggaa attatgtaat ccaacatgta ctggagcacg gtcgtcctga
     5641 ggataaaagc aaaattgtag cagaaatccg aggcaatgta cttgtattga gtcagcacaa
     5701 atttgcaagc aatgttgtgg agaagtgtgt tactcacgcc tcacgtacgg agcgcgctgt
     5761 gctcatcgac gaggtgtgca ccatgaacga cggtccccac agtgccttat acaccatgat
     5821 gaaggaccag tatgccaact acgtggtcca gaagatgatt gacgtggcgg agccaggcca
     5881 gcggaagatc gtcatgcata agatccggcc ccacatcgca actcttcgta agtacaccta
     5941 tggcaagcac attctggcca agctggagaa gtactacatg aagaacggtg ttgacttagg
     6001 ggacccaaag aagaagcgca aagtggatcc taaaaagaaa agaaaggtag gcggccgcgg
     6061 gggaggcggt tccggtggcg gcggaagcgg aggtggagga tcagggccgg ccgaactgcc
     6121 cacctgcagc tgtcttgatc gagttataca aaaagacaaa ggcccatatt atacacacct
     6181 tggggcagga ccaagtgttg ctgctgtcag ggaaatcatg gagaataggt atggtcaaaa
     6241 aggaaacgca ataaggatag aaatagtagt gtacaccggt aaagaaggga aaagctctca
     6301 tgggtgtcca attgctaagt gggttttaag aagaagcagt gatgaagaaa aagttctttg
     6361 tttggtccgg cagcgtacag gccaccactg tccaactgct gtgatggtgg tgctcatcat
     6421 ggtgtgggat ggcatccctc ttccaatggc cgaccggcta tacacagagc tcacagagaa
     6481 tctaaagtca tacaatgggc accctaccga cagaagatgc accctcaatg aaaatcgtac
     6541 ctgtacatgt caaggaattg atccagagac ttgtggagct tcattctctt ttggctgttc
     6601 atggagtatg tactttaatg gctgtaagtt tggtagaagc ccaagcccca gaagatttag
     6661 aattgatcca agctctccct tacatgaaaa aaaccttgaa gataacttac agagtttggc
     6721 tacacgatta gctccaattt ataagcagta tgctccagta gcttaccaaa atcaggtgga
     6781 atatgaaaat gttgcccgag aatgtcggct tggcagcaag gaaggtcgtc ccttctctgg
     6841 ggtcactgct tgcctggact tctgtgctca tccccacagg gacattcaca acatgaataa
     6901 tggaagcact gtggtttgta ccttaactcg agaagataac cgctctttgg gtgttattcc
     6961 tcaagatgag cagctccatg tgctacctct ttataagctt tcagacacag atgagtttgg
     7021 ctccaaggaa ggaatggaag ccaagatcaa atctggggcc atcgaggtcc tggcaccccg
     7081 ccgcaaaaaa agaacgtgtt tcactcagcc tgttccccgt tctggaaaga agagggctgc
     7141 gatgatgaca gaggttcttg cacataagat aagggcagtg gaaaagaaac ctattccccg
     7201 aatcaagcgg aagaataact caacaacaac aaacaacagt aagccttcgt cactgccaac
     7261 cttagggagt aacactgaga ccgtgcaacc tgaagtaaaa agtgaaaccg aaccccattt
     7321 tatcttaaaa agttcagaca acactaaaac ttattcgctg atgccatccg ctcctcaccc
     7381 agtgaaagag gcatctccag gcttctcctg gtccccgaag actgcttcag ccacaccagc
     7441 tccactgaag aatgacgcaa cagcctcatg cgggttttca gaaagaagca gcactcccca
     7501 ctgtacgatg ccttcgggaa gactcagtgg tgccaatgca gctgctgctg atggccctgg
     7561 catttcacag cttggcgaag tggctcctct ccccaccctg tctgctcctg tgatggagcc
     7621 cctcattaat tctgagcctt ccactggtgt gactgagccg ctaacgcctc atcagccaaa
     7681 ccaccagccc tccttcctca cctctcctca agaccttgcc tcttctccaa tggaagaaga
     7741 tgagcagcat tctgaagcag atgagcctcc atcagacgaa cccctatctg atgaccccct
     7801 gtcacctgct gaggagaaat tgccccacat tgatgagtat tggtcagaca gtgagcacat
     7861 ctttttggat gcaaatattg gtggggtggc catcgcacct gctcacggct cggttttgat
     7921 tgagtgtgcc cggcgagagc tgcacgctac cactcctgtt gagcacccca accgtaatca
     7981 tccaacccgc ctctcccttg tcttttacca gcacaaaaac ctaaataagc cccaacatgg
     8041 ttttgaacta aacaagatta agtttgaggc taaagaagct aagaataaga aaatgaaggc
     8101 ctcagagcaa aaagaccagg cagctaatga aggtccagaa cagtcctctg aagtaaatga
     8161 attgaaccaa attccttctc ataaagcatt aacattaacc catgacaatg ttgtcaccgt
     8221 gtccccttat gctctcacac acgttgcggg gccctataac cattgggtca tcgattgatt
     8281 aattaagaat tcgacccagc tttcttgtac aaagtggttg gtaagcctat ccctaaccct
     8341 ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg
     8401 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     8461 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     8521 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8581 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8641 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8701 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8761 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8821 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8881 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8941 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     9001 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     9061 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     9121 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9181 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9241 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     9301 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     9361 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9421 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9481 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     9541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9661 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     9721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     9781 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     9841 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     9901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    10021 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10081 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    10141 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    10201 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    10261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    10321 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    10381 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    10441 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    10501 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    10561 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    10621 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    10681 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    10741 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    10801 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    10861 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    10921 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    10981 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    11041 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    11101 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    11161 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    11221 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    11281 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    11341 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    11401 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    11461 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
