LOCUS       sequence-271790-       10268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z
  TITLE     Expanding C-T base editing toolkit with diversified cytidine 
            deaminases.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
  PUBMED    31399578
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3612. doi"; pages: " 
            10.1038/s41467-019-11562-6"
COMMENT     Sequence Label: N-4-BE
FEATURES             Location/Qualifiers
     source          1..10268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          168..1105
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1062..1082
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1820..1843,1844..1846,1847..5920)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1820..5920
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5921..5968
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5975..6223
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6236..6256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6272..6325
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6326..6328,6329..7039)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6326..6328,6329..7039)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6629..6648)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6976..6997
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7068..7085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7074..7281
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7290..7430
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7290..7419
                     /label=AAV2 ITR
     rep_origin      7505..7960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7592..7611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7802..7823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7977..7996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8096..8118
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8156..8174)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8242..8346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8347..8415,8416..9207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8565..8584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9378..9966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9867..9886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(10028..10268,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10028..10048
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10199..10218
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
       61 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      121 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      181 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      241 ccactggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
      301 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
      361 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
      421 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
      481 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
      541 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
      601 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
      661 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
      721 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
      781 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
      841 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
      901 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
      961 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     1021 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     1081 tcaaagtttt tttcttccat ttcaggtgtc gtgaggtgtc gtgagcgcca tggccggatc
     1141 cgccggcgac gagaacgtgc gcgtgagcga gaagctggac ttcaacacct tcgagttcga
     1201 gttcgagaac ctgcactacg ccgagggccg cggccgcacc tacgtgatct tcgacgtgaa
     1261 gccccagagc gagggcggcc gcggcaagcg cctgtggggc tacgtgcgca acaaccccct
     1321 ggacgaccac gccgaggtga tcctgatgag caagatcaac gaccacctgg agacacacca
     1381 gggcaactac accatgacct ggtacatgag ctggagcccc tgcggcaact gcagcagcga
     1441 gctggtgccc tggctgaaga acctggagga gcagcagcac accctgacca tgcacttcag
     1501 ccgcatctac gacaaggacc gcgccgtgga ccaccgcggc ctgtgcgacc tgcagcgcgt
     1561 ggtgagcaac tacttccaga tgggcgtgat gcgcaagaag gaggtgaaga agtgcctggc
     1621 cgagtacgtg gaggccagcg gccgcaccct gcgctggctg cgcaccacca ccagcaacgc
     1681 cggccgccgc cgccgcaagc tgtatagcat cctggtgcgc tgcgccggca tgcgcgagag
     1741 cggcatgccc ctgcacctgt tcaccgctag acgatccagc gagactcccg ggacctcaga
     1801 gtccgccaca ccccgatccg acaagaagta cagcatcggc ctggccatcg gcaccaactc
     1861 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     1921 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     1981 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2041 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2101 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2161 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2221 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2281 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2341 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2401 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2461 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2521 tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     2581 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     2641 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     2701 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     2761 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     2821 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     2881 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     2941 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     3001 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3061 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3121 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3181 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     3241 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3301 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3361 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3421 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3481 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3541 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     3601 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     3661 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     3721 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     3781 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     3841 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     3901 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     3961 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4021 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4081 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4141 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4201 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4261 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4321 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4381 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4441 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4501 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4561 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     4621 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     4681 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     4741 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     4801 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     4861 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     4921 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     4981 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5041 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5101 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5161 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     5221 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5281 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5341 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5401 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5461 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5521 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     5581 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     5641 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     5701 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     5761 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     5821 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     5881 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa
     5941 aaaggccggc caggcaaaaa agaaaaagga attcactaat ctgtcagata ttattgaaaa
     6001 ggaaaccggc aagcaactgg ttatccagga atccatcctc atgctcccag aggaggtgga
     6061 agaagtcatt gggaacaagc cggaaagcga tatactcgtg cacaccgcct acgacgagag
     6121 caccgacgag aatgtcatgc ttctgactag cgacgcccct gaatacaagc cttgggctct
     6181 ggtcatacag gatagcaacg gtgagaacaa gattaagatg ctctctggtg gttctcccaa
     6241 gaagaagagg aaagtcgcta gcggcagtgg agagggcaga ggaagtctgc taacatgcgg
     6301 tgacgtcgag gagaatcctg gcccagtgag caagggcgag gagctgttca ccggggtggt
     6361 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6421 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6481 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6541 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6601 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6661 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6721 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6781 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6841 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6901 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6961 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7021 catggacgag ctgtacaagg caattctaac tagagctcgc tgatcagcct cgactgtgcc
     7081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7201 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7261 gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc
     7321 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     7381 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     7441 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     7501 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7561 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     7621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7681 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     7741 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7801 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     7861 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     7921 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     7981 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     8041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     8161 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8221 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8281 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8341 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8401 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8461 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8521 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8581 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8641 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8701 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8761 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8821 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8881 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8941 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9001 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9061 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9121 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9181 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9241 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9301 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9361 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9421 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9481 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9541 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9601 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9661 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9721 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9781 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9841 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9901 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9961 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10021 cacatgtgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
    10081 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
    10141 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
    10201 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
    10261 gaaaggac
//
