LOCUS       sequence-271802-       11282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z
  TITLE     Expanding C-T base editing toolkit with diversified cytidine 
            deaminases.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
  PUBMED    31399578
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3612. doi"; pages: " 
            10.1038/s41467-019-11562-6"
COMMENT     Sequence Label: C-11-BE
FEATURES             Location/Qualifiers
     source          1..11282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          168..1105
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1062..1082
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1133..1198
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1205..1225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1250..1273,1274..1276,1277..5350)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1250..5350
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5351..5398
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5612..5677
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6701..6952
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6989..7237
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7250..7270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7286..7339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7340..7342,7343..8053)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7340..7342,7343..8053)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7643..7662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7990..8011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8082..8099)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8088..8295
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8304..8444
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8304..8433
                     /label=AAV2 ITR
     rep_origin      8519..8974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8606..8625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8816..8837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8991..9010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9110..9132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9170..9188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9256..9360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9361..9429,9430..10221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9579..9598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10392..10980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10881..10900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(11042..11282,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     11042..11062
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     11213..11232
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
       61 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      121 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      181 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      241 ccactggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
      301 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
      361 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
      421 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
      481 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
      541 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
      601 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
      661 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
      721 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
      781 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
      841 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
      901 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
      961 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     1021 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     1081 tcaaagtttt tttcttccat ttcaggtgtc gtgaggtgtc gtgagcgcca tggactataa
     1141 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
     1201 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta
     1261 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
     1321 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
     1381 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
     1441 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
     1501 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
     1561 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
     1621 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
     1681 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
     1741 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
     1801 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
     1861 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
     1921 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag
     1981 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
     2041 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
     2101 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
     2161 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
     2221 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
     2281 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg
     2341 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
     2401 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
     2461 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
     2521 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
     2581 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
     2641 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
     2701 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
     2761 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac
     2821 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     2881 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     2941 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     3001 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     3061 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     3121 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     3181 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     3241 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     3301 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     3361 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     3421 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     3481 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     3541 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     3601 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     3661 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
     3721 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag
     3781 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg
     3841 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca
     3901 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag
     3961 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
     4021 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
     4081 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
     4141 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
     4201 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
     4261 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
     4321 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
     4381 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
     4441 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa
     4501 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
     4561 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga
     4621 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
     4681 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
     4741 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
     4801 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
     4861 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
     4921 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
     4981 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
     5041 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
     5101 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
     5161 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
     5221 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
     5281 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
     5341 gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga
     5401 attgggtgga ggaggtacag gcggtggagg ctcagcagaa tacgtacgag ctctgtttga
     5461 cttcaatggg aatgacgagg aggatctccc ctttaagaag ggcgatattc tccgcatcag
     5521 agataagccc gaagaacaat ggtggaatgc cgaggatagc gaagggaaaa ggggcatgat
     5581 tctggtgcct tatgtggaga aatattccgg tgactacaaa gaccatgatg gggattacaa
     5641 agaccacgac atcgactaca aagacgacga cgataaagaa ttcggatcca gcgtgttcct
     5701 gcacaagaag ctgcccctga acaccttcct gttcgagttc gacaacctgg agaaggccta
     5761 cggccgcaac cgctgctaca tctgcttcaa gctgaagccc atctacgccg tgggcgccac
     5821 cggcaccacc ggcaccaccg gcagcgagct gtggggctac gccaccaaca agtgggaggt
     5881 ggccgacggc atcccccgcg agagccccga gaagcgcggc atgcacaccg aggagctgct
     5941 gctggacgag atgacccgcc acgtgcgcga gcacggcggc gccagcttct gcatcgagtg
     6001 gttcaccagc tggagcccct gccaccgctg cagcggcctg ctgcacagct ggctgcgcga
     6061 cgtgggcggc ggacgccacc gcctgcgcgt gtggttcagc cgcatctact acggcgacgt
     6121 gggcgccgtg cgcgccggcc tgcgccgcct gcgccgcgcc ggcgtgcagc tgggcgtgat
     6181 ggacggccgc ctgcgcgact actgcgccca cgccctggtg gacaccgccc agggcgaccc
     6241 cacccccctg tggctggtgc cctggcacat gaacgtgccc cgcgtgcagc gcgccttcga
     6301 cgagatcatg gacgagaagg acgacaacgg cagcgacaac agcgaccccg gcaagctgag
     6361 cgagagcatc agcggcggcc acgagagctg gcacgacgac gacctgcacc tgcccctgga
     6421 ggacctgacc gtggtggtgg agtgcacccc cagcaagcag ggcccccccg aggccaccgc
     6481 cgcccccacc ctgccccgca agcgccagca ggaggacccc gtggacgccc tgaccgccaa
     6541 gcgcgccctg ttcgtgcgcg agcccggctg gtgctggtgg tgctggcgcc cctggtggct
     6601 ggccgacgtg ctgagcaacg ccagcttccc ccagctgcac gtgaacgacg aggccatcgt
     6661 ggaccgcgtg gagaagttcc tggctagagg tacccaattc atgaccaacc tttccgacat
     6721 catagagaag gaaacaggca aacagttggt catccaagag tcgatactca tgcttcctga
     6781 agaagttgag gaggtcattg ggaataagcc ggaaagtgac attctcgtac acactgcgta
     6841 tgatgagagc accgatgaga acgtgatgct gctcacgtca gatgccccag agtacaaacc
     6901 ctgggctctg gtgattcagg actctaatgg agagaacaag atcaagatgc tatctggtgg
     6961 ttctggagga tctggtggtt ctcaattcac taatctgtca gatattattg aaaaggaaac
     7021 cggcaagcaa ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt
     7081 cattgggaac aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga
     7141 cgagaatgtc atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat
     7201 acaggatagc aacggtgaga acaagattaa gatgctctct ggtggttctc ccaagaagaa
     7261 gaggaaagtc gctagcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt
     7321 cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7381 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7441 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     7501 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     7561 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7621 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7681 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7741 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     7801 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7861 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7921 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7981 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     8041 cgagctgtac aaggcaattc taactagagc tcgctgatca gcctcgactg tgccttctag
     8101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8161 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     8221 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag
     8281 caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     8341 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     8401 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     8461 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     8521 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8581 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8641 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8701 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     8761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8821 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     8881 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8941 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     9001 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     9061 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     9121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     9181 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    10021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
    10081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10501 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    11041 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
    11101 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
    11161 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
    11221 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
    11281 ac
//
