LOCUS       sequence-271816-       12564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of Casilio-ME3.2 effector 
            PUFa-hNEIL2-hTET1(CD) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT611_CMV/CAG_PUFa-hNEIL2-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..12564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4769..4789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4817..4837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4928..4948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6005..6025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6029..6049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9365..9389)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9391..9432
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9474..10062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9527..9547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9945..9956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10134..10367
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10445..10566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10482..10501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10536..10555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10606..10741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10761..10780)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10762..10780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10790..10807)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10790..10806)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10799..10821)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10948..11403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11035..11054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11245..11266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11429..11533
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11534..11602,11603..12394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11752..11771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgatcgat ggcggaggcg gatctgatcc aaaaaagaag agaaaggtag atccaaaaaa
     4801 gaagagaaag gtagatccaa aaaagaagag aaaggtagga tctaccggat ctagaaacga
     4861 tggtggtggt ggaagcgggg gtgggggcag cggtggaggg ggaagcgggc gcgccgggat
     4921 cctccccccc aagaaaaaga ggaaggtatc tagaggccgc agccgccttt tggaagattt
     4981 tcgaaacaac cggtacccca atttacaact gcgggagatt gctggacata taatggaatt
     5041 ttcccaagac cagcatgggt ccagattcat tcagctgaaa ctggagcgtg ccacaccagc
     5101 tgagcgccag cttgtcttca atgaaatcct ccaggctgcc taccaactca tggtggatgt
     5161 gtttggtaat tacgtcattc agaagttctt tgaatttggc agtcttgaac agaagctggc
     5221 tttggcagaa cggattcgag gccacgtcct gtcattggca ctacagatgt atggcagccg
     5281 tgttatcgag aaagctcttg agtttattcc ttcagaccag cagaatgaga tggttcggga
     5341 actagatggc catgtcttga agtgtgtgaa agatcagaat ggcaatcacg tggttcagaa
     5401 atgcattgaa tgtgtacagc cccagtcttt gcaatttatc atcgacgcgt ttaagggaca
     5461 ggtatttgcc ttatccacac atccttatgg ctgccgagtg attcagagaa tcctggagca
     5521 ctgtctccct gaccagacac tccctatttt agaggagctt caccagcaca cagagcagct
     5581 ggtacaggat caatatggaa attatgtaat ccaacatgta ctggagcacg gtcgtcctga
     5641 ggataaaagc aaaattgtag cagaaatccg aggcaatgta cttgtattga gtcagcacaa
     5701 atttgcaagc aatgttgtgg agaagtgtgt tactcacgcc tcacgtacgg agcgcgctgt
     5761 gctcatcgac gaggtgtgca ccatgaacga cggtccccac agtgccttat acaccatgat
     5821 gaaggaccag tatgccaact acgtggtcca gaagatgatt gacgtggcgg agccaggcca
     5881 gcggaagatc gtcatgcata agatccggcc ccacatcgca actcttcgta agtacaccta
     5941 tggcaagcac attctggcca agctggagaa gtactacatg aagaacggtg ttgacttagg
     6001 ggacccaaag aagaagcgca aagtggatcc taaaaagaaa agaaaggtag gcggccgcgg
     6061 cggaggttct ggtggaggat caggtggagg gagtggtgga ggaagtgggg gaggttctgg
     6121 aggtggatcg ttgccagaag ggccgttggt gaggaaattt caccatttgg tctccccctt
     6181 tgtgggtcag caggtggtca agacaggggg cagcagtaag aagctacagc ccgccagcct
     6241 gcagtctctg tggctccagg acacccaggt ccatggaaag aaattattcc ttagatttga
     6301 tctagatgaa gaaatggggc cccctggcag cagcccaaca ccagagcctc cacaaaaaga
     6361 agtgcagaag gaaggggctg cggacccaaa gcaggtcggg gagcccagcg ggcagaagac
     6421 ccttgatgga tcctcacggt ctgcagagct cgtcccccag ggcgaggatg attctgagta
     6481 tttggagaga gacgcccctg caggagatgc tgggaggtgg ctgcgtgtca gctttggttt
     6541 gtttggcagc gtttgggtga acgatttctc cagagccaag aaagccaaca agagggggga
     6601 ctggagggac ccttccccga ggttggtcct gcactttggt ggtggtggct tcctggcatt
     6661 ttataattgt cagttgtctt ggagctcttc cccagtggtc acacccacct gtgacatcct
     6721 gtctgagaag ttccatcgag gacaagcctt agaagctcta ggccaggctc agcctgtctg
     6781 ctatacactg ctggaccaga gatacttctc agggctaggg aacatcatta agaatgaagc
     6841 cttgtacaga gctgggatcc atcccctttc tctcggttca gtcctgagtg cctcgcgtcg
     6901 ggaggtcctg gtggatcacg tggtggagtt cagtacagcc tggctgcagg gcaagttcca
     6961 aggcagaccg cagcacacac aggtctacca gaaagaacag tgccctgctg gccaccaggt
     7021 catgaaggag gcgtttgggc ccgaagatgg gttacagagg ctcacctggt ggtgcccgca
     7081 gtgccagccc cagttgtcag aggagccaga gcagtgccag ttctcgcggg gtggaggcgg
     7141 ttccggtggc ggcggaagcg gaggtggagg atcagggccg gccgaactgc ccacctgcag
     7201 ctgtcttgat cgagttatac aaaaagacaa aggcccatat tatacacacc ttggggcagg
     7261 accaagtgtt gctgctgtca gggaaatcat ggagaatagg tatggtcaaa aaggaaacgc
     7321 aataaggata gaaatagtag tgtacaccgg taaagaaggg aaaagctctc atgggtgtcc
     7381 aattgctaag tgggttttaa gaagaagcag tgatgaagaa aaagttcttt gtttggtccg
     7441 gcagcgtaca ggccaccact gtccaactgc tgtgatggtg gtgctcatca tggtgtggga
     7501 tggcatccct cttccaatgg ccgaccggct atacacagag ctcacagaga atctaaagtc
     7561 atacaatggg caccctaccg acagaagatg caccctcaat gaaaatcgta cctgtacatg
     7621 tcaaggaatt gatccagaga cttgtggagc ttcattctct tttggctgtt catggagtat
     7681 gtactttaat ggctgtaagt ttggtagaag cccaagcccc agaagattta gaattgatcc
     7741 aagctctccc ttacatgaaa aaaaccttga agataactta cagagtttgg ctacacgatt
     7801 agctccaatt tataagcagt atgctccagt agcttaccaa aatcaggtgg aatatgaaaa
     7861 tgttgcccga gaatgtcggc ttggcagcaa ggaaggtcgt cccttctctg gggtcactgc
     7921 ttgcctggac ttctgtgctc atccccacag ggacattcac aacatgaata atggaagcac
     7981 tgtggtttgt accttaactc gagaagataa ccgctctttg ggtgttattc ctcaagatga
     8041 gcagctccat gtgctacctc tttataagct ttcagacaca gatgagtttg gctccaagga
     8101 aggaatggaa gccaagatca aatctggggc catcgaggtc ctggcacccc gccgcaaaaa
     8161 aagaacgtgt ttcactcagc ctgttccccg ttctggaaag aagagggctg cgatgatgac
     8221 agaggttctt gcacataaga taagggcagt ggaaaagaaa cctattcccc gaatcaagcg
     8281 gaagaataac tcaacaacaa caaacaacag taagccttcg tcactgccaa ccttagggag
     8341 taacactgag accgtgcaac ctgaagtaaa aagtgaaacc gaaccccatt ttatcttaaa
     8401 aagttcagac aacactaaaa cttattcgct gatgccatcc gctcctcacc cagtgaaaga
     8461 ggcatctcca ggcttctcct ggtccccgaa gactgcttca gccacaccag ctccactgaa
     8521 gaatgacgca acagcctcat gcgggttttc agaaagaagc agcactcccc actgtacgat
     8581 gccttcggga agactcagtg gtgccaatgc agctgctgct gatggccctg gcatttcaca
     8641 gcttggcgaa gtggctcctc tccccaccct gtctgctcct gtgatggagc ccctcattaa
     8701 ttctgagcct tccactggtg tgactgagcc gctaacgcct catcagccaa accaccagcc
     8761 ctccttcctc acctctcctc aagaccttgc ctcttctcca atggaagaag atgagcagca
     8821 ttctgaagca gatgagcctc catcagacga acccctatct gatgaccccc tgtcacctgc
     8881 tgaggagaaa ttgccccaca ttgatgagta ttggtcagac agtgagcaca tctttttgga
     8941 tgcaaatatt ggtggggtgg ccatcgcacc tgctcacggc tcggttttga ttgagtgtgc
     9001 ccggcgagag ctgcacgcta ccactcctgt tgagcacccc aaccgtaatc atccaacccg
     9061 cctctccctt gtcttttacc agcacaaaaa cctaaataag ccccaacatg gttttgaact
     9121 aaacaagatt aagtttgagg ctaaagaagc taagaataag aaaatgaagg cctcagagca
     9181 aaaagaccag gcagctaatg aaggtccaga acagtcctct gaagtaaatg aattgaacca
     9241 aattccttct cataaagcat taacattaac ccatgacaat gttgtcaccg tgtcccctta
     9301 tgctctcaca cacgttgcgg ggccctataa ccattgggtc atcgattgat taattaagaa
     9361 ttcgacccag ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt
     9421 ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac
     9481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9841 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     9901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    10021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
    10081 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10141 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
    10201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
    10381 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    10441 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10561 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
    10621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10741 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    10801 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    10861 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    10921 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    10981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    11041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    11101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    11161 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    11221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    11281 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    11341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    11401 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    11461 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    11521 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    11581 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    11641 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    11701 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    11761 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    11821 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    11881 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    11941 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    12001 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    12061 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    12121 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    12181 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    12241 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    12301 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    12361 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    12421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    12481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    12541 cgtagaaaag atcaaaggat cttc
//
