LOCUS       sequence-271839-       10323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of Casilio-ME3.4 effector 
            hNEIL2-PUFc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT596_CMV/CAG_hNEIL2-PUFc
FEATURES             Location/Qualifiers
     source          1..10323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5762..5782
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5786..5806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5810..5830
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5921..5941
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6998..7018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7022..7042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(7124..7148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7150..7191
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7233..7821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7286..7306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7704..7715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7893..8126
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8204..8325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8241..8260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8295..8314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8365..8500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8520..8539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8521..8539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8549..8566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8549..8565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8558..8580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8707..9162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8794..8813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9004..9025
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9188..9292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9293..9361,9362..10153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9511..9530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgccagaa gggccgttgg tgaggaaatt tcaccatttg gtctccccct ttgtgggtca
     4801 gcaggtggtc aagacagggg gcagcagtaa gaagctacag cccgccagcc tgcagtctct
     4861 gtggctccag gacacccagg tccatggaaa gaaattattc cttagatttg atctagatga
     4921 agaaatgggg ccccctggca gcagcccaac accagagcct ccacaaaaag aagtgcagaa
     4981 ggaaggggct gcggacccaa agcaggtcgg ggagcccagc gggcagaaga cccttgatgg
     5041 atcctcacgg tctgcagagc tcgtccccca gggcgaggat gattctgagt atttggagag
     5101 agacgcccct gcaggagatg ctgggaggtg gctgcgtgtc agctttggtt tgtttggcag
     5161 cgtttgggtg aacgatttct ccagagccaa gaaagccaac aagagggggg actggaggga
     5221 cccttccccg aggttggtcc tgcactttgg tggtggtggc ttcctggcat tttataattg
     5281 tcagttgtct tggagctctt ccccagtggt cacacccacc tgtgacatcc tgtctgagaa
     5341 gttccatcga ggacaagcct tagaagctct aggccaggct cagcctgtct gctatacact
     5401 gctggaccag agatacttct cagggctagg gaacatcatt aagaatgaag ccttgtacag
     5461 agctgggatc catccccttt ctctcggttc agtcctgagt gcctcgcgtc gggaggtcct
     5521 ggtggatcac gtggtggagt tcagtacagc ctggctgcag ggcaagttcc aaggcagacc
     5581 gcagcacaca caggtctacc agaaagaaca gtgccctgct ggccaccagg tcatgaagga
     5641 ggcgtttggg cccgaagatg ggttacagag gctcacctgg tggtgcccgc agtgccagcc
     5701 ccagttgtca gaggagccag agcagtgcca gttctccatc gatggcggag gcggatctga
     5761 tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa
     5821 gagaaaggta ggatctaccg gatctagaaa cgatggtggt ggtggaagcg ggggtggggg
     5881 cagcggtgga gggggaagcg ggcgcgccgg gatcctcccc cccaagaaaa agaggaaggt
     5941 atctagaggc cgcagccgcc ttttggaaga ttttcgaaac aaccggtacc ccaatttaca
     6001 actgcgggag attgctggac atataatgga attttcccaa gaccagcatg ggtccagatt
     6061 cattcagctg aaactggagc gtgccacacc agctgagcgc cagcttgtct tcaatgaaat
     6121 cctccaggct gcctaccaac tcatggtgga tgtgtttggt aattacgtca ttcagaagtt
     6181 ctttgaattt ggcagtcttg aacagaagct ggctttggca gaacggattc gaggccacgt
     6241 cctgtcattg gcactacaga tgtatggcag ccgtgttatc gagaaagctc ttgagtttat
     6301 tccttcagac cagcagaatg agatggttcg ggaactagat ggccatgtct tgaagtgtgt
     6361 gaaagatcag aatggcaatc acgtggttca gaaatgcatt gaatgtgtac agccccagtc
     6421 tttgcaattt atcatcgacg cgtttaaggg acaggtattt gccttatcca cacatcctta
     6481 tggctgccga gtgattcaga gaatcctgga gcactgtctc cctgaccaga cactccctat
     6541 tttagaggag cttcaccagc acacagagca gctggtacag gatcaatatg gaagttatgt
     6601 aatcgaacat gtactggagc acggtcgtcc tgaggataaa agcaaaattg tagcagaaat
     6661 ccgaggcaat gtacttgtat tgagtcagca caaatttgca aacaatgttg tgcagaagtg
     6721 tgttactcac gcctcacgta cggagcgcgc tgtgctcatc gacgaggtgt gcaccatgaa
     6781 cgacggtccc cacagtgcct tatacaccat gatgaaggac cagtatgcca actacgtggt
     6841 ccagaagatg attgacgtgg cggagccagg ccagcggaag atcgtcatgc ataagatccg
     6901 gccccacatc gcaactcttc gtaagtacac ctatggcaag cacattctgg ccaagctgga
     6961 gaagtactac atgaagaacg gtgttgactt aggggaccca aagaagaagc gcaaagtgga
     7021 tcctaaaaag aaaagaaagg taggcggccg cgggggaggc ggttccggtg gcggcggaag
     7081 cggaggtgga ggatcagggc cggcctgatt aattaagaat tcgacccagc tttcttgtac
     7141 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     7201 gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     7261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7621 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7741 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7801 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     7861 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7921 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7981 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8041 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8101 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     8161 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8341 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8401 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8461 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     8521 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8581 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8641 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8701 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8761 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8821 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8881 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8941 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     9001 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     9061 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9121 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     9181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9301 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9361 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9421 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9481 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9541 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9601 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9661 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9721 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9781 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9841 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9901 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9961 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10021 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10081 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10141 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10201 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10321 ttc
//
