LOCUS       sequence-271863-        5106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert 
            T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, 
            Nero Moffatt J, Salih A, Royant A, Shaner NC
  TITLE     Aequorea's secrets revealed: New fluorescent proteins with unique 
            properties for bioimaging and biosensing.
  JOURNAL   PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov.
  PUBMED    33137097
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-11-2"; volume: "18(11):e3000936. doi"; pages: " 
            10.1371/journal.pbio.3000936. eCollection 2020 Nov"
COMMENT     Sequence Label: H2B-mAvicFP1
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             650..1027
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1040..1049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1887..2008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1924..1943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1978..1997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2015..2470)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2152..2173)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2364..2383
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2497..2601
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2603..2960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2603..2794
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2626..2646)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2811..2946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2995..3789
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2995..3789
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3049..3068)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3659..3678
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3977..3996)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4021..4068
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4397..4985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4886..4905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      601 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgccacca tgccagagcc
      661 agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa
      721 gaaaggcggc aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa
      781 ggttctgaag caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa
      841 ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta
      901 caacaagcgc tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc
      961 tggggagttg gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag
     1021 cgctaaggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaacggtat
     1081 cgttcctatc ctgatcgaac tgaacggtga cgttcatggt cataagttct ctgttcgtgg
     1141 tgaaggtgaa ggtgacgctg gttctggtaa gatcgaaatc aagttcgttt gcaccaccgg
     1201 taccctgcct gttccttggc ctaccctggt taccacctta tgctacggtg ttcagtgctt
     1261 cacccgttac cctgaacata tgaagcagca tgacttctac aagtctgcta tgcctgacgg
     1321 ttacatccag aagcgtacca tctctttcca ggacgacggt cattacaaga cccgtgctga
     1381 agttaagttc gaaggtgaca ccctggttaa ccgtatcgaa ctgaagggta tcgacttcaa
     1441 ggaagacggt aacatcctgg gtaacaagat ggaatacaac tacaactctc attctgttta
     1501 cgttctgtct gacaaggcta acaacggtat caaggttaac ttcaagatcc gtcataacct
     1561 gaagggtgaa ggtatccagc tggctgacca tgaccagcag aacatcccta tcggtgacgg
     1621 tcctgttctg ctgcctgact accattacct gtctacccag accaagatca ccaaggaccc
     1681 taacgaaaag cgtgaccata tgaacctggt tgaattcgtt accgcttgcg gtatcaccca
     1741 tggtatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
     1801 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1861 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1981 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     2041 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     2101 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     2161 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     2221 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     2281 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     2341 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     2401 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     2461 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2521 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2581 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     2641 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2701 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2761 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2821 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2881 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2941 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3001 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3061 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3121 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     3181 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3241 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3301 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3361 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     3421 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     3481 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     3541 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     3601 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     3661 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     3721 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     3781 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     3841 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     3901 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     3961 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4021 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     4081 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4141 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4201 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     4261 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg ggggtcgtgc acacagccca
     4741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     5101 agttat
//
