LOCUS       sequence-271902-        7585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal myc-tagged mouse KIF5B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Twelvetrees AE, Yuen EY, Arancibia-Carcamo IL, MacAskill AF, 
            Rostaing P, Lumb MJ, Humbert S, Triller A, Saudou F, Yan Z, Kittler 
            JT
  TITLE     Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and 
            disrupted by mutant huntingtin.
  JOURNAL   Neuron. 2010 Jan 14;65(1):53-65. doi: 10.1016/j.neuron.2009.12.007.
  PUBMED    20152113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.12"; journalName: "Neuron"; date: 
            "2010-01-14- 14"; volume: "65"; issue: "1"; pages: "53-65"
COMMENT     Sequence Label: myc-KIF5B
FEATURES             Location/Qualifiers
     source          1..7585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        70..449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     604..624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     650..674
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        884..902
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     884..901
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     915..934
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      981..990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             990..1019
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3941..3950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3954..4088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3991..4010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4045..4064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4157..4514
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4157..4348
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4180..4200)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4365..4500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4534..4551)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4534..4550)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4543..4565)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4763..5218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4850..4869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5060..5081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5235..5254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5354..5376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5414..5432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5500..5604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5605..5673,5674..6465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5823..5842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6636..7224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7125..7144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7378..7395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7512..7533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7548..7553,7554..7571,7572..7578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg
       61 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      661 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      721 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      781 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
      841 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
      901 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
      961 cggttctatc gattgaattg gccaccatgg agcagaagct gatctccgag gaggacctgg
     1021 gatccatggc ggacccggcg gagtgcaaca tcaaagtgat gtgtcgcttc agacctctca
     1081 acgaatctga agtgaaccgc ggcgataagt acgtcgccaa attccaggga gaagacacgg
     1141 tgatgatcgc gtccaagcct tatgcctttg atcgtgtgtt ccagtcaagc acatctcaag
     1201 agcaagtata caacgactgt gcaaaaaaga ttgttaaaga tgttcttgag ggctataatg
     1261 gaacaatatt tgcatatgga caaacatcat ctgggaagac ccacacgatg gagggtaaac
     1321 ttcatgatcc agaaggcatg ggaattattc caagaatagt gcaagatatt tttaattata
     1381 tttactccat ggatgaaaat ttggaatttc atattaaggt ttcatatttt gaaatatatt
     1441 tggataagat aagggacttg ttagatgttt caaagactaa cctttcagtc catgaagaca
     1501 aaaaccgtgt tccctatgta aaggggtgca cagaacgttt cgtgtgtagt ccagatgaag
     1561 tcatggatac catagatgaa gggaaatcca acagacatgt cgcagttaca aatatgaatg
     1621 aacatagctc taggagtcac agcatatttc ttattaatgt aaaacaagag aatacacaaa
     1681 cggaacagaa actcagtgga aagctttatc tggttgattt agctggcagt gagaaggtta
     1741 gtaagactgg ggctgaaggt gctgtgctgg atgaagctaa gaacatcaac aagtcacttt
     1801 ctgcacttgg aaatgtcatt tctgctttgg cagagggcag tacctatgtt ccttatcgag
     1861 atagtaaaat gaccagaatt cttcaagatt cattaggtgg caactgtagg accactattg
     1921 tcatatgctg ctctccatca tcatacaatg agtctgagac aaagtcaaca ctcctctttg
     1981 gtcaaagggc caaaacaatt aagaacacag tctgtgtcaa tgtagagtta actgcggagc
     2041 agtggaaaaa gaagtatgaa aaagaaaagg aaaaaaataa aactctacgg aacactattc
     2101 agtggctgga aaacgagcta aaccgttggc gtaacgggga gacagtgcct attgatgagc
     2161 agtttgacaa agagaaagct aatttggaag ccttcacagc ggataaagat attgctatta
     2221 ccagtgataa accagctgct gcagtcggaa tggctggtag ttttaccgat gctgaaagaa
     2281 gaaagtgtga agaagaactt gctaaattgt ataaacaact tgatgacaag gatgaagaga
     2341 ttaaccaaca aagccaattg gtagagaaat tgaagacaca aatgctggat caggaagagc
     2401 ttctggcatc aaccagaagg gatcaagata atatgcaagc tgaactgaat cgcctccaag
     2461 cagaaaatga tgcttctaaa gaagaagtca aagaagtttt acaggcctta gaggaactgg
     2521 ctgttaatta tgatcagaag tctcaggaag ttgaagacaa aacaaaggaa tatgaattgc
     2581 ttagtgatga attgaatcaa aaatctgcaa ctttagcaag tattgatgct gagcttcaga
     2641 agctgaagga aatgaccaac caccagaaga aacgagcagc tgaaatgatg gcatcattat
     2701 taaaagacct tgcagaaata ggaattgctg tggggaataa cgatgtgaag caaccagaag
     2761 gaactggtat gatagatgaa gagtttactg ttgcaagact ctacattagc aaaatgaaat
     2821 cagaagtaaa gaccatggtg aaacgctgca aacagctaga aagcacacag actgagagca
     2881 acaaaaaaat ggaagaaaat gagaaagagt tagcagcatg ccagcttcgg atctcccaac
     2941 atgaagccaa aatcaagtca ctgactgagt accttcagaa tgtagaacaa aagaagaggc
     3001 agctggagga gtctgttgat tcccttggtg aggagctagt ccaactccga gcacaagaga
     3061 aagtccatga aatggaaaaa gagcacttga acaaggttca gactgcaaat gaagtcaagc
     3121 aagctgttga gcagcagatc cagagtcaca gagaaaccca ccaaaaacaa atcagtagtt
     3181 tgcgagatga agtggaggca aaggaaaagc taatcactga cctccaagac caaaaccaga
     3241 agatggtgtt ggagcaggaa cggctaaggg tggagcatga gaggctgaag gctacagacc
     3301 aagagaagag caggaagctg cacgagctca cggttatgca agacagacga gaacaagcaa
     3361 gacaagactt gaagggtttg gaggagaccg tggcaaaaga acttcagact ttacacaacc
     3421 tgcgtaagct ctttgttcag gacttggcta ccagggtgaa aaagagcgcc gaggtcgact
     3481 ctgacgacac tggcggcagt gctgcacaga agcagaaaat ctccttcctt gaaaacaacc
     3541 ttgaacagct caccaaagtg cacaagcagt tggtacgcga taatgcagat cttcgctgtg
     3601 agcttcctaa gttagagaaa cggcttagag ctactgcaga aagagtgaaa gctttggagt
     3661 cagccctgaa agaagccaaa gaaaatgcat ctcgagaccg taaacgctat cagcaagaag
     3721 tagaccggat aaaggaagca gtcaggtcaa agaacatggc cagaagggga cattctgccc
     3781 agattgcaaa gccgatccgt cctggacagc atccagcggc ctcgccaact cacccgggca
     3841 cagttcgtgg aggaggctca tttgttcaga acaaccagcc agtggggctt cgtggtggtg
     3901 gaggcaagca gtcgtaatct agaattcctg cagaagcttg gccgccatgg cccaacttgt
     3961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4081 tctggatcgg gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa
     4141 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     4201 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4261 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4321 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4381 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4441 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4501 taggcttttg caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga
     4561 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4621 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4681 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4741 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4981 ggtgatggtc cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5101 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5221 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5281 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5341 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5401 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5461 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5581 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5641 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5701 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5761 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5821 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5881 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5941 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6001 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6061 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6121 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6181 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6241 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6301 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6361 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6421 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6481 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6781 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7381 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7441 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7501 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7561 cttccggctc gtatgttgtg tggaa
//
