LOCUS       sequence-271904-        6465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Miro1 with the transmembrane deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Lopez-Domenech G, Covill-Cooke C, Ivankovic D, Halff EF, Sheehan DF,
            Norkett R, Birsa N, Kittler JT
  TITLE     Miro proteins coordinate microtubule- and actin-dependent 
            mitochondrial transport and distribution.
  JOURNAL   EMBO J. 2018 Feb 1;37(3):321-336. doi: 10.15252/embj.201696380. Epub
            2018 Jan 8.
  PUBMED    29311115
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201696380"; journalName: "EMBO J"; date: "2018-02-1- 
            1"; volume: "37"; issue: "3"; pages: "321-336"
COMMENT     Sequence Label: myc-Miro1 (lacking TM)
FEATURES             Location/Qualifiers
     source          1..6465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        435..453
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     435..452
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     466..485
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             541..570
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2372..2381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2385..2519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2422..2441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2476..2495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2588..2945
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2588..2779
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2611..2631)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2796..2931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2965..2982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2965..2981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2974..2996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3194..3649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3281..3300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3491..3512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3666..3685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3785..3807
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3845..3863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3931..4035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4036..4104,4105..4896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4254..4273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5067..5655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5556..5575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5809..5826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5943..5964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5979..5984,5985..6002,6003..6009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6017..6033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6022..6044
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6041..6057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6041..6057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6086..6465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
      301 cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc
      361 tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat aaccttatgt
      421 atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc tttctctcca
      481 caggtgtcca ctcccaggtc caactgcacc tcggttctat cgattgaatt ggccaccatg
      541 gagcagaagc tgatctccga ggaggacctg ggatctatga agaaagacgt gcggatcctg
      601 ctggtgggag aacctagagt tgggaagaca tcactgatta tgtctctggt cagtgaagaa
      661 tttccagaag aggttcctcc ccgggcagaa gaaatcacca ttccagctga tgtcacccca
      721 gagagagttc caacacacat tgtagattac tcagaagcag aacagagtga tgaacaactt
      781 catcaagaaa tatctcaggc taatgtcatc tgtatagtgt atgccgttaa caacaagcat
      841 tctattgata aggtaacaag tcgatggatt cctctcataa atgaaagaac agacaaagac
      901 agcaggctgc ctttaatatt ggttgggaac aaatctgatc tggtggaata tagtagtatg
      961 gagaccatcc ttcctattat gaaccagtat acagaaatag aaacctgtgt ggagtgttca
     1021 gcgaaaaacc tgaagaacat atcagagctc ttttattacg cacagaaagc tgttcttcat
     1081 cctacagggc ccctgtactg cccagaggag aaggagatga aaccagcttg tataaaagcc
     1141 cttactcgta tatttaaaat atctgatcaa gataatgatg gtactctcaa tgatgctgaa
     1201 ctcaacttct ttcagaggat ttgtttcaac actccattag ctcctcaagc tctggaggat
     1261 gtcaagaatg tagtcagaaa acatataagt gatggtgtgg ctgacagtgg gttgaccctg
     1321 aaaggttttc tctttttaca cacacttttt atccagagag ggagacacga aactacttgg
     1381 actgtgcttc gacgatttgg ttatgatgat gacctggatt tgacacctga atatttgttc
     1441 cccctgctga aaatacctcc tgattgcact actgaattaa atcatcatgc atatttattt
     1501 ctccaaagca cctttgacaa gcatgatttg gatagagact gtgctttgtc acctgatgag
     1561 cttaaagatt tatttaaagt tttcccttac ataccttggg ggccagatgt gaataacaca
     1621 gtttgtacca atgaaagagg ctggataacc taccagggat tcctttccca gtggacgctc
     1681 acgacttatt tagatgtaca gcggtgcctg gaatatttgg gctatctagg ctattcaata
     1741 ttgactgagc aagagtctca agcttcagct gttacagtga caagagataa aaagatagac
     1801 ctgcagaaaa aacaaactca aagaaatgtg ttcagatgta atgtaattgg agtgaaaaac
     1861 tgtgggaaaa gtggagttct tcaggctctt cttggaagaa acttaatgag gcagaagaaa
     1921 attcgtgaag atcataaatc ctactatgcg attaacactg tttatgtata tggacaagag
     1981 aaatacttgt tgttgcatga tatctcagaa tcggaatttc taactgaagc tgaaatcatt
     2041 tgtgatgttg tatgcctggt atatgatgtc agcaatccca aatcctttga atactgtgcc
     2101 aggattttta agcaacactt tatggacagc agaatacctt gcttaatcgt agctgcaaag
     2161 tcagacctgc atgaagttaa acaagaatac agtatttcac ctactgattt ctgcaggaaa
     2221 cacaaaatgc ctccaccaca agccttcact tgcaatactg ctgatgcccc cagtaaggat
     2281 atctttgtta aattgacaac aatggccatg tatccgcacg tgacacaagc tgacctcaag
     2341 agctccacgt tttgattcct gcagaagctt ggccgccatg gcccaacttg tttattgcag
     2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
     2521 ggaattaatt cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     2581 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2941 gcaaaaagct gttaacagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3001 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3061 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3121 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3181 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3241 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3301 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3361 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3421 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     3541 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3661 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3721 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3781 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3841 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5761 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5821 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5881 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5941 attaatgtga gttagctcac tcattaggca ccccagtctt tacactttat gcttccggct
     6001 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     6061 attacgaatt aattcgagct cgcccgacat tgattattga ctagttatta atagtaatca
     6121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
