LOCUS       sequence-271918-        7020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged Myosin-19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Lopez-Domenech G, Covill-Cooke C, Ivankovic D, Halff EF, Sheehan DF,
            Norkett R, Birsa N, Kittler JT
  TITLE     Miro proteins coordinate microtubule- and actin-dependent 
            mitochondrial transport and distribution.
  JOURNAL   EMBO J. 2018 Feb 1;37(3):321-336. doi: 10.15252/embj.201696380. Epub
            2018 Jan 8.
  PUBMED    29311115
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201696380"; journalName: "EMBO J"; date: "2018-02-1- 
            1"; volume: "37"; issue: "3"; pages: "321-336"
COMMENT     Sequence Label: GFP-Myo19
FEATURES             Location/Qualifiers
     source          1..7020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3444..3565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3481..3500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3535..3554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3572..4027)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3709..3730)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3921..3940
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4054..4158
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4160..4517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4160..4351
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4183..4203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4368..4503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4552..5346
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4552..5346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4606..4625)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5216..5235
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5534..5553)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5578..5625
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5954..6542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6443..6462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6717..7020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtccgg actcagatct atgctccagc aggtcaatgg ccacaatccg gggtctgatg
     1021 gccaagccag ggagtacctc agagaagacc tgcaggagtt cctgggtggg gaggtcctgc
     1081 tgtacaaact ggatgacctc accagggtga atcctgtgac actagagaca gtcctgaggt
     1141 gcctgcaggc ccggtacatg gcagacacat tctacaccaa tgctggctgc accctggtag
     1201 ccttgaaccc cttcaagcct gttcctcagc tctactcgcc cgagctaatg agagagtacc
     1261 atgctgcgcc tcagccccag aaactgaagc cccatgtgtt cactgtgggt gaacagacct
     1321 acaggaatgt caagagcctg attgaaccag tcaaccagtc tattgttgtc agtggagaga
     1381 gtggtgctgg aaagacatgg acgtctcgct gcctaatgaa gttctatgct gtggtggcca
     1441 cctcacctgc atcttgggag agccacaaga ttgcagagag gatagaacag aggatcctga
     1501 actccaaccc tgtcatggaa gcttttggga atgcgtgtac actgaggaat aacaacagca
     1561 gtcgctttgg gaagttcatc cagctccagc tgaacagggc tcagcaaatg actggagccg
     1621 cagtccagac ctacctccta gagaaaactc gagtggcctg ccaggcttcc agtgagagga
     1681 acttccacat cttctatcag atttgcaaag gagccagtga ggacgagagg ctccagtggc
     1741 accttcctga gggagctgcc ttctcctggc tgcccaaccc agagaggagc ttagaagagg
     1801 attgttttga ggtgaccaga gaggccatgc tccatttggg cattgacacc cctacccaga
     1861 acaacatctt taaggtccta gctggactgc tgcaccttgg caatatccag tttgctgcct
     1921 ccgaggatga agcccagccc tgccagccga tggatgatgc caagtactct gtcaggacgg
     1981 cagcctcgct gctggggctc ccagaggacg tgctgctgga gatggtgcag attagaacca
     2041 tcagggcagg cagacagcag caggtgttcc ggaagccctg cgcccgagcc gagtgtgaca
     2101 cccgtagaga ctgcctggcc aaactgatct atgcgcggtt gtttgactgg ctggtatcag
     2161 tgatcaacag cagcatctgt gcagacaccg actcgtggac cactttcata ggcctgctgg
     2221 atgtgtatgg atttgaatca tttcctgaca acagtctgga acagttgtgc atcaactacg
     2281 ccaatgagaa gctgcagctg gaggcctgtg gcctcgtgga gaccatccat atcagtgctg
     2341 ctggcttccc catccgggtc tctcaccgaa actttgtaga acgatacaag ttactaagaa
     2401 ggcttcatcc ttgcacatcc tctggccccg acagcccata tcctgccaaa gggctccctg
     2461 aatggtgtcc acacagcgag gaagccacgc ttgaacctct catccaggac attctccaca
     2521 ctctgccggt cctaactcag gcagcagcca taactggtga ctcggctgag gccatgccag
     2581 cccccatgca ctgtggcagg accaaggtgt tcatgactga ctctatgctg gagcttctgg
     2641 aatgtgggcg tgcccgggtg ctggagcagt gtgcccgctg catccagggt ggctggaggc
     2701 gacaccggca ccgagagcag gagcggcagt ggcgggccgt catgctcatc caggcagcca
     2761 ttcgttcctg gttaactcgg aaacacatcc agaggctgca tgcagctgcc acagtcatca
     2821 agcgtgcatg gcagaagtgg agaatcagaa tggcctgcct tgctgctaaa gagctggatg
     2881 gtgtggaaga aaaacacttc tctcaagctc cctgttccct gagcacctcg ccgctgcaga
     2941 ccaggctcct ggaggcaata atccgcctct ggcccctggg actggtcctg gccaatacgg
     3001 ctatgggtgt aggcagcttt cagaggaaat tagtggtctg ggcttgcctc cagctcccca
     3061 ggggcagccc cagtagctac actgtccaga cagcacaaga ccaggctggt gtcacgtcca
     3121 tccgagcgct gcctcaggga tcgataaagt ttcactgcag aaagtctcca ctgcggtatg
     3181 ctgacatctg ccctgaacct tcaccctaca gcattacagg ctttaatcag attctgctgg
     3241 aaagacacag gctgatccac gtgacctctt ctgccttcac tgggctgggg tgagtcgacg
     3301 gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     3361 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3421 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3481 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3541 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     3601 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3661 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3721 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3781 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3841 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3901 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3961 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4021 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4141 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4201 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4261 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4321 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4501 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     4561 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4621 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4681 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     4741 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4801 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4861 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4921 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4981 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5041 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     5101 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5161 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5221 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5281 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5341 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5401 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5461 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     5521 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     5581 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     5641 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     5701 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     5761 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5821 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5881 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6061 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6601 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     6661 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6721 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6781 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6841 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6901 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6961 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
