LOCUS       sequence-271921-        5600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal myc-tagged mouse Pex19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Covill-Cooke C, Toncheva VS, Drew J, Birsa N, Lopez-Domenech G, 
            Kittler JT
  TITLE     Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, 
            Miro1 and Miro2.
  JOURNAL   EMBO Rep. 2020 Feb 5;21(2):e49865. doi: 10.15252/embr.201949865. 
            Epub 2020 Jan 2.
  PUBMED    31894645
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2020-02-5"; volume: "21(2):e49865. doi"; pages: " 
            10.15252/embr.201949865. Epub 2020 Jan 2"
COMMENT     Sequence Label: myc-Pex19
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      912..921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             921..950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      955..964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1887..1896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1900..2034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1937..1956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1991..2010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2103..2460
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2103..2294
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2126..2146)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2311..2446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2480..2497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2480..2496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2489..2511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2709..3164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2796..2815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3006..3027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3181..3200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3300..3322
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3360..3378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3446..3550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3551..3619,3620..4411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3769..3788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4582..5170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5071..5090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5324..5341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5458..5479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5494..5499,5500..5517,5518..5524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5532..5548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5537..5559
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5556..5572
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5556..5572
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt ggccaccatg gagcagaagc tgatctccga ggaggacctg ggatccatgg
      961 cggctgctga ggaaggttgc ggtgttgggg tcgaagacga ccgggaactg gaggagcttc
     1021 tggaaagtgc tcttgatgat ttcgacaaag ccaaaccctc cccagaacat gctccgacca
     1081 tctcggctcc cgacgcttca ggaccccaga agagagcgcc aggagatact gccaaagatg
     1141 ctctcttcgc ctcccaagag aaatttttcc aggaactgtt tgacagtgag ctggcttccc
     1201 aagctactgc ggagtttgag aaggcaatga aggagctggc tgaggaagag ccccatctgg
     1261 tggagcagtt ccagaagctc tcagaggcag ctgggagagt gggcagcgat gcaagttctc
     1321 agcaagagtt tacttcttgc ctaaaggaga cgttaagtgg cctggccaaa aacgccactg
     1381 agctgcagaa ctcgggcatg tctgaagagg agctgatgaa agccatggaa gggctgggca
     1441 tggatgaggg ggatggggaa gcgagcattc tccccatcat gcagagcatc atgcagaacc
     1501 tcctgtctaa ggatgtgctg tacccatccc tgaaggagat cacagaaaag tatccagaat
     1561 ggctccagag tcaccaggac tccactcctc ccgagcagtt tgagaagtac cagcagcagc
     1621 acagcgtcat ggtcaaaatc tgtgagcagt ttgaggccga gacgcccaca gacagcgagg
     1681 ctactcagag ggctcgcttt gaggccatgc tagatctcat gcagcagtta caggccttgg
     1741 gccatcctcc aaaagagctg gctggggaga tgcctcctgg cctcaacttt gacctggatg
     1801 ctctcaatct gtcgggcccc ccaggtgcta atggcgaaca gtgtctgatc atggaattcc
     1861 tgcagaagct tgtcgaccct gcaggggccg ccatggccca acttgtttat tgcagcttat
     1921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgggaat
     2041 taattcggcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     2101 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2281 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2341 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2401 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2461 aagctgttaa cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2521 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2581 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     2641 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2701 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2761 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2821 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2881 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     2941 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3001 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     3061 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3121 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5461 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5521 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     5581 gaattaattc gagctcgccc
//
