LOCUS       sequence-271938-        3341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of C. elegans KNL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Kern DM, Kim T, Rigney M, Hattersley N, Desai A, Cheeseman IM
  TITLE     The outer kinetochore protein KNL-1 contains a defined 
            oligomerization domain in nematodes.
  JOURNAL   Mol Biol Cell. 2015 Jan 15;26(2):229-37. doi: 
            10.1091/mbc.E14-06-1125. Epub 2014 Nov 19.
  PUBMED    25411336
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-06-1125"; journalName: "Mol Biol Cell"; date: 
            "2015-01-15- 15"; volume: "26"; issue: "2"; pages: "229-37"
COMMENT     Sequence Label: pDK038
FEATURES             Location/Qualifiers
     source          1..3341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     693..712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     946..963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1048..1067
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1048..1066
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1098..1120
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1140..1157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1770..1788)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1784..1831
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1928..2383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2015..2034)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2225..2246
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2409..2513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2514..2582,2583..3341,1..33)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2732..2751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
       61 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      841 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      901 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      961 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1021 taatgcagga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
     1081 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc
     1141 atcatcatca tcatcatggt atggctagca ctgacactat tggatcagat ggtattttga
     1201 caccacgtgg cggaaacatg gatatttccg aatctccggc ctgcacgtcc tcatttcaag
     1261 tgttcggcgg tggtaatctc gataaaacta tggatatgtc tctcgaaaca actatcaacg
     1321 agaacaacga aacggcgaga ttgtttgaaa ccacaagaga tccaacacta ttatacgaaa
     1381 agatcgtcga aaccacaaca aaagttaccg agcgaattgt tagtatgcca ctggatgata
     1441 ccttagcaat gttcaataca acgaatcaag aagataagga tatgtcagtt gatcgttcag
     1501 ttcttttcac gattcccaaa gttccgaagc ataacgctac aatgaataga actataccga
     1561 tggacctcga tgaatcaaaa gcagcgggcg gccagtgcga tgaaacgatg aatgtgttca
     1621 atttcacaaa cttggaagcc gctgaaatgg atacgagtaa attagatgaa tagggatccg
     1681 agctcgagat ctgcagctgg taccatggaa ttcgaagctt gatccggctg ctaacaaagc
     1741 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1801 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatctgg
     1861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1921 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1981 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2041 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2101 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2161 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2221 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2281 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2341 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     2401 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2461 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2521 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2581 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2641 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2701 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2761 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2821 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2881 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2941 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3001 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3061 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3121 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3181 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3241 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3301 ggagtcaggc aactatggat gaacgaaata gacagatcgc t
//
