LOCUS       sequence-271964-        5043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Tamai K, Zeng X, Liu C, Zhang X, Harada Y, Chang Z, He X
  TITLE     A mechanism for Wnt coreceptor activation.
  JOURNAL   Mol Cell. 2004 Jan 16. 13(1):149-56.
  PUBMED    14731402
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jan 16. 13(1):149-56."
COMMENT     Sequence Label: LRP6 deltaN-pCS2-VSVG
FEATURES             Location/Qualifiers
     source          1..5043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        935..953
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     935..952
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1082..1114
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     polyA_signal    1995..2129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2019..2038)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2073..2092
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2242..2262)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2242..2260)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2281..2297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2281..2297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2294..2316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2305..2321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2329..2335,2336..2353,2354..2359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2512..2529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2683..3271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2763..2782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3442..4233,4234..4302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4065..4084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4303..4407)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4433..4888)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4570..4591)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4782..4801
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(4959..5043,1..900)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
       61 acttggcacc atgccaattc aatatggcgg acctggcact gtgccaactg gggaggggtc
      121 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
      181 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
      241 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
      301 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
      361 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
      421 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
      481 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
      541 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
      601 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
      661 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg
      721 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
      781 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
      841 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
      901 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
      961 ctacttgttc tttttgcagg atccagagaa gagaacgcga gaagggaaga tgggggccgt
     1021 cctgaggagc ctcctggcct gcagcttctg tgtgctcctg agagcggccc ctttgttgct
     1081 ttatactgat atcgaaatga accgcctggg taaggctccg gaggccacca atacagttgg
     1141 ttctgttatt ggcgtaattg tcaccatttt tgtgtctgga actgtatact ttatctgcca
     1201 gaggatgttg tgtccacgta tgaagggaga tggggaaact atgactaatg actatgtagt
     1261 tcatggacca gcttctgtgc ctcttggtta tgtgccacac ccaagttctt tgtcaggatc
     1321 tcttccagga atgtctcgag gtaaatcaat gatcagctcc ctcagtatca tggggggaag
     1381 cagtggaccc ccctatgacc gagcccatgt tacaggagca tcatcaagta gttcttcaag
     1441 caccaaaggc acttacttcc ctgcaatttt gaaccctcca ccatccccag ccacagagcg
     1501 atcacattac actatggaat ttggatattc ttcaaacagt ccttccactc ataggtcata
     1561 cagctacagg ccatatagct accggcactt tgcacccccc accacaccct gcagcacaga
     1621 tgtttgtgac agtgactatg ctcctagtcg gagaatgacc tcagtggcaa cagccaaggg
     1681 ctataccagt gacttgaact atgattcaga acctgtgccc ccacctccca caccccgaag
     1741 ccaatacttg tcagcagagg agaactatga aagctgccca ccttctccat acacagagag
     1801 gagctattct catcacctct acccaccgcc accctctccc tgtacagact cctcctgagg
     1861 aggggccctc ctcctctgac tgcctccaac gtaaaaatgt aaatataaat ttggttgaga
     1921 tctggagggg gggagggagc tattagagaa ggatgaggca gaccatctag aactatagtg
     1981 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2041 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2101 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2161 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg
     2221 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2341 cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3121 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     4441 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     4501 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     4561 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4621 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4681 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4741 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4801 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4861 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     4921 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg
     4981 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
     5041 cgt
//
