LOCUS       sequence-271973-        2974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Lucia Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pLucia
FEATURES             Location/Qualifiers
     source          1..2974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     350..369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     564..580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     564..580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        596..614
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     596..613
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1328..1347)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1329..1347)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1354..1371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1354..1370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1390..1845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1527..1548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1739..1758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1958..2617)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2532..2551
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2618..2720)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      join(2835..2974,1..449)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
       61 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      541 ataaccgtgt catgatgcct ggacaggaaa cagctatgac caacggctct atggtattta
      601 ggtgacacta tagaacgaat tcactagtga ttgggcgatg agtggtctcg aatggagatc
      661 aaggttctgt tcgctctgat ctgtattgct gttgccgagg ccaagccgac agagataaat
      721 gaggacctca acattgcagc cgtagcctcc aacttcgcca caacggacct cgagacagac
      781 ttgtttacga attgggagac tatgaacgtt atatccaccg atactgagca agtgaatacg
      841 gacgctgacc gtggcaagct ccctggaaag aaattgcctc ctgatgtcct gcgtgaactc
      901 gaagcgaatg cgcgtcgcgc aggttgtact cgcggttgcc tcatatgcct gagccatatc
      961 aaatgtacac ctaaaatgaa gaaatttatt ccgggacgtt gtcatacgta tgagggtgaa
     1021 aaggaaagcg cacagggtgg cataggagag gctatcgttg acatccctga aataccaggt
     1081 tttaaggaca aggaaccatt ggaccagttt attgcccaag tggacctctg tgccgactgc
     1141 acgacgggtt gtctgaaggg tctggctaat gtccaatgca gcgatttgct caaaaagtgg
     1201 ctgccgcaac gctgtacgac ttttgcatcc aagatccagg gacgagtcga taagatcaag
     1261 ggattggctg gagatcggtg agcttcgaga ccttgagatc aatatctaag gtcctaagcg
     1321 gaattcaccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac atgcgtttct
     1381 acaatgttaa aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     1441 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     1501 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     1561 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgctggccca ctacgtgaac
     1621 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     1681 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     1741 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     1801 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaacat catgtttgac
     1861 agcttatcat cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac
     1921 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     1981 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2041 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2101 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2161 aactcaccca gggattggct gacacgaaaa acatattctc aataaaccct ttagggaaat
     2221 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2281 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2341 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     2401 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2461 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2521 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2581 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2641 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2701 aacctcttac gtgccgatca tcacaaaagg atctaggtga agatcctttt tgataatctc
     2761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2941 aaggtaactg gcttcagcag agcgcagata ccaa
//
