LOCUS       sequence-271974-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AP-1 GreenRenilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: 6xAP-1_RE::GrRenilla
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    974..1198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1318..1906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1398..1417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2021..2123
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2124..2939
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2124..2939
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2197..2216)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3053..3508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3140..3159)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3350..3371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3533..3581
                     /note="synthetic polyadenylation signal"
     misc_feature    3595..3686
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3635..3654
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3809..3840
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
ORIGIN
        1 cttggcaatc cggtactgtt ggtaaagcca ccaatggctt ccaaagtcta cgacccagaa
       61 caaaggaaga ggatgattac tggaccgcag tggtgggcaa ggtgcaagca gatgaatgta
      121 ctcgatagtt ttatcaatta ttacgactcc gaaaaacacg cggaaaatgc tgtcatattc
      181 ctgcacggga acgccactag cagctacttg tggcggcacg tagtccccca catagagcct
      241 gttgcccgat gtattattcc cgatctcatt ggaatgggca aatcaggcaa atctgggaac
      301 ggttcttacc gcctgctcga tcactataaa tatttgaccg cttggtttga gctgcttaat
      361 ctccctaaaa aaataatatt cgttggacac gactggggct cagctctggc gttccactac
      421 gcatacgagc atcaagaccg aataaaagca atagtacata tggagtcagt tgttgacgtc
      481 atagagtcat ggatgggttg gccagatatt gaagaggaat tggccctcat caagagtgag
      541 gagggggaaa aaatggtcct ggaaaataat tttttcgtgg aaaccttgct cccgagtaaa
      601 ataatgcgaa aacttgaacc cgaagagttt gctgcttatt tggagccttt caaggaaaag
      661 ggggaagtaa gacgaccaac cctttcttgg ccgagggaga tacctcttgt taagggtgga
      721 aagcccgacg tagtccagat agtccgaaat tacaacgcgt acctgagagc tagtgatgac
      781 ctccctaagt tgtttatcga gtcagaccct gggttcttca gcaacgcaat tgtagaagga
      841 gcgaaaaaat ttccaaatac agaatttgtg aaggttaagg ggctccactt tttgcaagag
      901 gacgcccccg atgagatggg aaaatacatt aaatctttcg tagagcgcgt ccttaagaat
      961 gaacagtgag cttctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1021 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1081 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1141 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggcg
     1201 cttgagacga tatctgacac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1501 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1741 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1981 gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac gtaagaggtt
     2041 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     2101 tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct tgctcaaggc
     2161 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2221 tcgggcaatc aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt
     2281 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     2341 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     2401 atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat
     2461 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2521 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgactc gctcaggcgc
     2581 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2641 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2701 tagtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2761 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2821 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2881 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctgat
     2941 tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat
     3001 aataagcgga tgaatggcag aaattcgatg ataagctgtc aaacatgatg ttacgcgccc
     3061 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3121 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3181 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3241 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccagcgccc
     3301 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3361 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3421 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3481 tttaacaaaa tattaacgct tacaatttta acgatatcgt ctcatggcgg agaataaaat
     3541 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     3601 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     3661 aagtgcaggt gccagaacat ttctctcgct gcagactatg agtcaaacag cgtgactcaa
     3721 gttgagtcag ttgactcact tgagtcactg actcaggcat gctccctcga ggatatcaaa
     3781 tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag
//
