LOCUS       sequence-271977-        4883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM673
FEATURES             Location/Qualifiers
     source          1..4883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(38..43,44..60,61..66))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(137..170)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        247..338
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     263..280
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             381..1034
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     992..1014
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1041..1064
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    2576..2710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2613..2632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2667..2686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2831..2996
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3186..3774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3675..3694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3909..4132
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4199..4732)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
       61 tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
      121 cagcagcgaa gtcgccataa cttcgtatag catacattat acgaagttat ctgtaactat
      181 aacggtccta aggtagcgag tttaaacact agtatcgatt cgcgacctac tccggaatat
      241 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
      301 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
      361 ccaccatcgg gcgcggatct atgtccccta tactaggtta ttggaaaatt aagggccttg
      421 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
      481 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
      541 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
      601 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
      661 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
      721 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
      781 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
      841 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
      901 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
      961 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
     1021 accatcctcc aaaatcggat ctggaagttc tgttccaggg gcccctggga tccgaagaag
     1081 tgacttcaac cgttaccaaa agccgccgta ttagtcgtcg tcctagcgat tggtgggttg
     1141 tgaaaagcga agaaagcccg gtgtatagca attctagcgt ccgtaatgag ctgcctatgc
     1201 accataacag ttcccgtaaa agcaccaaaa aaaccaacca gtctagcaaa aacattcgta
     1261 aaaaaacgat ccctctgaaa cgccagaaaa ccgccacaaa aggcaaccaa cgtgttcaga
     1321 aattcctgaa cgccgaagga tcaggaggca ttgttgggca cgatgagatt agtcgctgct
     1381 ccctgtcaga acctctggaa tccgatgaag ccgatctggc taaaaaaaaa aacctggact
     1441 gctcccgttc tacccgtagc agtaaaaatg aggataacat catgaccgcc cagaatgtcc
     1501 cactgaaacc tcagacaagc ggttatacct gtaacattcc gaccgaaagc aatctggaca
     1561 gcggtgagca caaaacaagt gtcctggaag agtctggtcc tagtcgtctg aataacaatt
     1621 atctgatgtc cgggaaaaat gatgtggacg atgaggaagt tcacggtagt agcgacgatt
     1681 caaaacagag taaagtcatc cctaaaaatc gtattcacca taaactggtg ctgccgagta
     1741 atactccgaa cgttcgtcgt acaaaacgta cacgcctgaa acctctggaa tattggcgtg
     1801 gtgaacgtat cgactaccag ggtcgtccat ctggtggttt tgtaatcagc ggtgtgctga
     1861 gtcctgatac gattagcagc aaacgtaaag ccaaagagaa catcggcaaa gtcaacaaaa
     1921 aatccaacaa aaaacgcatc tgcctggata acgatgagcg taaaacgaat ctgatggtga
     1981 atctgggtat tcctctggga gatcctctgc agcctacccg tgtaaaagat cctgagacac
     2041 gtgagattat cctgatggac ctggttcgtc ctcaagacac ctatcagttt ttcgtcaaac
     2101 acggcgagct gaaagtctat aaaaccctgg acaccccgtt ctttagtacc ggcaaactga
     2161 ttctgggtcc tcaggaggaa aaaggcaaac agcacgtggg tcaagacatc ctggtctttt
     2221 atgtgaattt tggggacctg ctgtgtactc tgcatgaaac cccgtatatt ctgtctaccg
     2281 gtgacagctt ctatgtgccg tctggaaact attataacat caaaaacctg cgcaacgaag
     2341 aaagtgttct gctgttcaca cagatcaaac gctgagaatt caaaggccta cgtcgacgag
     2401 ctcacttgtc gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcgagaa
     2461 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     2521 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     2581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2701 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     2761 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
     2821 atctaattgg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     2881 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     2941 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg
     3001 gttgacttgg gtcaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3841 cctgcgttat cccctgattg acttgggtcg ctcttcctgt ggatgcgcag atgccctgcg
     3901 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     3961 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     4021 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     4081 tgcccgtcgt attaaagagg ggcgtggcca agggcatgta aagactatat tcgcggcgtt
     4141 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
     4201 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     4261 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     4321 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     4381 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     4441 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     4501 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     4561 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     4621 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     4681 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     4741 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     4801 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     4861 ccgctgcgtt cggtcaaggt tct
//
