LOCUS       sequence-271979-        4396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of  CENP-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM692
FEATURES             Location/Qualifiers
     source          1..4396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(48..53,54..70,71..76))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(147..180)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        257..348
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     273..290
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             391..1044
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1002..1024
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1051..1074
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    2099..2233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2136..2155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2190..2209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2354..2519
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2709..3297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3198..3217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3432..3655
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3722..4255)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
       61 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
      121 caaccttggg cagcagcgaa gtcgccataa cttcgtatag catacattat acgaagttat
      181 ctgtaactat aacggtccta aggtagcgag tttaaacact agtatcgatt cgcgacctac
      241 tccggaatat taatagatca tggagataat taaaatgata accatctcgc aaataaataa
      301 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat
      361 tcataccgtc ccaccatcgg gcgcggatct atgtccccta tactaggtta ttggaaaatt
      421 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
      481 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
      541 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
      601 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
      661 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
      721 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
      781 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
      841 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
      901 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
      961 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
     1021 ggtggtggcg accatcctcc aaaatcggat ctggaagttc tgttccaggg gcccctggga
     1081 tcccaggagc gtaaaccttc tggttcatct cagaaccgta tccgtgatag cgaatatgag
     1141 attcaacgtc aggccaaaaa atcctttagc acgctgtttc tggagacagt gaaacgtaaa
     1201 agcgagtcta gcccgattgt tcgtcacgcc gctacagcac ctccacacag ttgtccaccg
     1261 gatgatacca aactaatcga agacgaattc atcattgacg agagcgacca gtcatttgcc
     1321 agtcgttctt ggattaccat tcctcgtaaa gccggttcac tgaaacaacg taccattagc
     1381 ccggcagaat caacagcact gttccaaggc cgtaaaagtc gtgaaaaaca ccacaatatc
     1441 ctgccgaaaa cactggccaa cgataaacac agccacaaac cacacccggt cgagacaagt
     1501 caaccatcag acaaaaccgt actggacacc tcttatgccc tgattgacga gacagtcaac
     1561 aactatcgta gtaccaaata tgaaatgtat agtaaaaatg ccgaaaaacc gagtcgtagc
     1621 aaacgtacca tcaaacaaaa acagcgccgt aaattcatgg caaaaccggc cgaagaacag
     1681 ctggatgttg ggcagagcaa agatgagaac attcacacgt cccatattac ccaagacgag
     1741 tttcagcgta acagtgaccg taacatggaa gaacacgaag aaatgggcaa cgattgtgtg
     1801 tcgaaaaaac aaatgccgcc ggtgggttca aaaaaatcga gtacccgtaa agacaaagaa
     1861 gagagcaaaa aaaaacgctt tagcagtgag tccaaaaaca aactggtgcc gtgagtcgac
     1921 gagctcactt gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca agcttgtcga
     1981 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2041 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     2101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2221 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     2281 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata
     2341 cccatctaat tggaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     2401 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     2461 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacaa
     2521 ccggttgact tgggtcaact gtcagaccaa gtttactcat atatacttta gattgattta
     2581 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2821 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3361 tttcctgcgt tatcccctga ttgacttggg tcgctcttcc tgtggatgcg cagatgccct
     3421 gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
     3481 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
     3541 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
     3601 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat gtaaagacta tattcgcggc
     3661 gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt
     3721 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact
     3781 ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag
     3841 caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc
     3901 tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa
     3961 acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa
     4021 gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt
     4081 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
     4141 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
     4201 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg
     4261 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     4321 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     4381 ccaccgctgc gttcgg
//
