LOCUS       sequence-271980-        5098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM672
FEATURES             Location/Qualifiers
     source          1..5098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(100..105,106..122,123..128))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(199..232)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        309..400
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     325..342
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             443..1096
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1054..1076
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1103..1126
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    2853..2987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2890..2909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2944..2963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3108..3273
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3463..4051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3952..3971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4186..4409
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4476..5009)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
       61 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
      121 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
      181 ggcagcagcg aagtcgccat aacttcgtat agcatacatt atacgaagtt atctgtaact
      241 ataacggtcc taaggtagcg agtttaaaca ctagtatcga ttcgcgacct actccggaat
      301 attaatagat catggagata attaaaatga taaccatctc gcaaataaat aagtatttta
      361 ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt attcataccg
      421 tcccaccatc gggcgcggat ctatgtcccc tatactaggt tattggaaaa ttaagggcct
      481 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
      541 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
      601 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
      661 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
      721 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
      781 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
      841 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
      901 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
      961 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     1021 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     1081 cgaccatcct ccaaaatcgg atctggaagt tctgttccag gggcccctgg gatccatggc
     1141 agcaagtggt ctggatcacc tgaaaaacgg ctatcgtcgt cgcttctgtc gcccaagtcg
     1201 tgctcgtgac attaacaccg aacagggcca gaatgtgctg gaaatcctgc aggactgctt
     1261 tgaagagaaa agcctggcga acgattttag caccaattcc acaaaaagtg tgccgaatag
     1321 tacccgtaaa atcaaagaca cgtgtatcca gtctccgtcc aaagaatgcc aaaaaagcca
     1381 cccgaaaagt gtaccagtga gtagcaaaaa aaaagaggcc tcgctgcaat ttgttgtcga
     1441 accgagcgaa gcaaccaacc gtagtgttca agcccatgaa gttcatcaga aaatcctggc
     1501 gacggatgtt tcaagcaaaa acaccccgga ctccaaaaaa atcagcagcc gtaacattaa
     1561 tgaccaccac tctgaagcgg atgaagagtt ctatctgagc gtgggttctc ctagcgttct
     1621 gctggacgcc aaaacctctg ttagccaaaa cgtgattccg agtagcgcca aaaaacgtga
     1681 gacatatacc ttcgagaata gcgtcaacat gctgccgtca tcaacagaag tgtcggtcaa
     1741 aacgaaaaaa cgcctgaatt tcgatgacaa agtgatgctg aaaaaaatcg agatcgataa
     1801 taaagtctct gacgaggagg ataaaacctc tgaaggacag gagcgtaaac cttctggttc
     1861 atctcagaac cgtatccgtg atagcgaata tgagattcaa cgtcaggcca aaaaatcctt
     1921 tagcacgctg tttctggaga cagtgaaacg taaaagcgag tctagcccga ttgttcgtca
     1981 cgccgctaca gcacctccac acagttgtcc accggatgat accaaactaa tcgaagacga
     2041 attcatcatt gacgagagcg accagtcatt tgccagtcgt tcttggatta ccattcctcg
     2101 taaagccggt tcactgaaac aacgtaccat tagcccggca gaatcaacag cactgttcca
     2161 aggccgtaaa agtcgtgaaa aacaccacaa tatcctgccg aaaacactgg ccaacgataa
     2221 acacagccac aaaccacacc cggtcgagac aagtcaacca tcagacaaaa ccgtactgga
     2281 cacctcttat gccctgattg acgagacagt caacaactat cgtagtacca aatatgaaat
     2341 gtatagtaaa aatgccgaaa aaccgagtcg tagcaaacgt accatcaaac aaaaacagcg
     2401 ccgtaaattc atggcaaaac cggccgaaga acagctggat gttgggcaga gcaaagatga
     2461 gaacattcac acgtcccata ttacccaaga cgagtttcag cgtaacagtg accgtaacat
     2521 ggaagaacac gaagaaatgg gcaacgattg tgtgtcgaaa aaacaaatgc cgccggtggg
     2581 ttcaaaaaaa tcgagtaccc gtaaagacaa agaagagagc aaaaaaaaac gctttagcag
     2641 tgagtccaaa aacaaactgg tgccgtgagt cgacgagctc acttgtcgcg gccgctttcg
     2701 aatctagagc ctgcagtctc gacaagcttg tcgagaagta ctagaggatc ataatcagcc
     2761 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2821 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2881 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2941 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg
     3001 agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     3061 gctgagcaat aactatcata acccctaggg tatacccatc taattggaac cagataagtg
     3121 aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac
     3181 ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc
     3241 cctcccagtt cccaactatt ttgtccgccc acaaccggtt gacttgggtc aactgtcaga
     3301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4081 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattgact
     4141 tgggtcgctc ttcctgtgga tgcgcagatg ccctgcgtaa gcgggtgtgg gcggacaata
     4201 aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga
     4261 atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta
     4321 tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc
     4381 gtggccaagg gcatgtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc
     4441 gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata
     4501 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg
     4561 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg
     4621 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga
     4681 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga
     4741 gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc
     4801 aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac
     4861 tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta
     4921 catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc
     4981 tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5041 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacag
//
