LOCUS       sequence-271982-       17004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for integration into the human AAVS1 safe harbor locus 
            of Doxicycline-inducible KRAB-dCas9-IRES-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D,
            Eggan K, Barrett LE
  TITLE     A multiplexed gRNA piggyBac transposon system facilitates efficient 
            induction of CRISPRi and CRISPRa in human pluripotent stem cells.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
  PUBMED    31959800
REFERENCE   2  (bases 1 to 17004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):635. doi"; pages: " 
            10.1038/s41598-020-57500-1"
COMMENT     Sequence Label: pT077
FEATURES             Location/Qualifiers
     source          1..17004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..352
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             434..619
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             join(641..667,668..670,671..3157,3158..3160,3161..4744)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             641..4744
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4748..4774
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4754..4780
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4820..4840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(4922..5496,5497..5499,5500..5508)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5089..5106)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5316..5335
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5509..5511,5512..5514,5515..6228)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5815..5834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6162..6183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6272..6860
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6325..6345)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6743..6754)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6863..6879)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    6965..7189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7190..7223
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        7308..7611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          7858..8875
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8799..8821
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8883..8902
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8941..9690
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPGS"
     polyA_signal    9699..9923
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10238..10257
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    10256..10311
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     10357..10376
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(11264..11320)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    11344..11369
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    11383..12186
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     repeat_region   12650..12712
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12842..12858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12851..12873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13000..13455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13087..13106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13297..13318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13481..13585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13586..13654,13655..14446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13804..13823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14617..15205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15106..15125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15359..15376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15493..15514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15529..15534,15535..15552,15553..15559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15567..15583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15572..15594
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15591..15607
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15591..15607
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   16098..16132
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    16142..16978
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
       61 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
      121 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
      181 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
      241 agagaacgta tgtcgaggta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac ttacgcgtct
      361 gcaggatatc aagcttgcgg taccgcgggc ccgggatcgc accatggatg ctaagtcact
      421 aactgcctgg tcccggacac tggtgacctt caaggatgta tttgtggact tcaccaggga
      481 ggagtggaag ctgctggaca ctgctcagca gatcgtgtac agaaatgtga tgctggagaa
      541 ctataagaac ctggtttcct tgggttatca gcttactaag ccagatgtga tcctccggtt
      601 ggagaaggga gaagagcccg gcggttccgg cggagggtcg atggacaaga agtacagcat
      661 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      721 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      781 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac
      841 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      901 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      961 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
     1021 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     1081 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     1141 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     1201 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     1261 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
     1321 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
     1381 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     1441 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     1501 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     1561 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     1621 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     1681 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc
     1741 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     1801 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     1861 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     1921 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     1981 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     2041 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag
     2101 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     2161 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt
     2221 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc
     2281 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     2341 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     2401 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     2461 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     2521 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     2581 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     2641 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     2701 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     2761 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     2821 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     2881 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     2941 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     3001 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     3061 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     3121 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct
     3181 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag
     3241 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct
     3301 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg
     3361 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     3421 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga
     3481 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     3541 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     3601 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     3661 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     3721 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     3781 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     3841 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct
     3901 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     3961 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga
     4021 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     4081 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     4141 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     4201 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     4261 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     4321 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     4381 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac actacctgga
     4441 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     4501 ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag agcaggccga
     4561 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     4621 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     4681 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     4741 cgacgcctat ccctatgacg tgcccgatta tgccagcctg ggcagcggct cccccaagaa
     4801 aaaacgcaag gtggaagatc ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag
     4861 caacggcagc agcggatcct aagcggccgc tcgataagct tgatatcgaa ttccgccccc
     4921 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4981 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5041 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5101 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5161 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5221 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5281 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5341 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5401 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5461 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag
     5521 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5581 ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg ccacctacgg caagctgacc
     5641 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5701 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5761 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5821 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5881 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5941 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg
     6001 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     6061 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     6121 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6181 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac tagtcctgca
     6241 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     6301 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6361 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6421 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6481 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6541 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6601 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6661 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6721 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6781 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6841 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
     6901 tacctttaag accgatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6961 ttaactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7021 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7081 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7141 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga taacttcgta
     7201 taatgtatgc tatacgaagt tatgcccggg caaggatctg cgatcgctag ttattaatag
     7261 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7321 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     7381 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     7441 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     7501 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     7561 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     7621 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     7681 ttatttattt tttaattatt ttgtgcagcg atgggggcgg ggcggggcga ggggcggggc
     7741 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     7801 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     7861 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     7921 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     7981 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     8041 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     8101 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     8161 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     8221 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     8281 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     8341 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     8401 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     8461 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     8521 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     8581 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     8641 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     8701 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     8761 ggggacggct gccttcgggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     8821 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     8881 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgc ggccgccacc
     8941 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatggagtc
     9001 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     9061 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     9121 gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     9181 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     9241 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     9301 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     9361 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     9421 cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag
     9481 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     9541 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     9601 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     9661 tttgaccttg acatgctccc cgggtcatag gtttaaacct gtgccttcta gttgccagcc
     9721 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9781 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     9841 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     9901 tggggatgcg gtgggctcta tggcttaagg atctccataa gagaagaggg acagctatga
     9961 ctgggagtag tcaggagagg aggaaaaatc tggctagtaa aacaggtaag gaaaatttta
    10021 gggatgttaa agaaaaaaat aacacaaaac aaaatataaa aaaaatctaa cctcaagtca
    10081 aggcttttct atggaataag gaatggacag cagggggctg tttcatatac tgatgacctc
    10141 tttatagcca acctttgttc atggcagcca gcatatgggc atatgttgcc aaactctaaa
    10201 ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt ccgagtgaga
    10261 gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat tagccagaag
    10321 tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga aaaagatcac
    10381 agtggtattt gtgagccagg gcattggcca caccagccac caccttctga taggcagcct
    10441 gcacctgagg agtgttaacc tattaaaaga actcatccag cagtctgtaa aaagcaatcc
    10501 tctgtgaatc aggtgcggca atcccataca gcaccaggaa cctatcggcc cattccccgc
    10561 ccagttcctc tgcaatgtct cttgtggcca gggcgatgtc ctggtatctg tcggcgacgc
    10621 ccagtcgtcc acaatcaata aacccactga atctgccatt ctccaccatg atgttgggca
    10681 ggcaagcgtc gccatgggtg accaccagat cttctccgtc tggcattctg gctttcagcc
    10741 gtgcaaacag ctctgcagga gccagcccct ggtgttcctc atccagatcg tcctggtcca
    10801 ccagcccggc ctccattcgg gttcgagccc tctcaatgcg atgtttagcc tggtgatcga
    10861 aagggcaggt ggcggggtcc agagtatgca gtctccgcat ggcatctgcc atgatggaga
    10921 ccttctcagc tggtgccagg tgggagctca gcaggtcctg tcctggcact tctcccagca
    10981 gcagccaatc tcgcccggcc tcagtgacca cgtccaggac tgcagcacaa ggcactcctg
    11041 tggtggccag ccagctcagg cgggctgctt catcctgcag ctcgttcagt gctcctgaca
    11101 ggtctgtctt gacaaacagc acggggcgcc cctgagcact cagccggaac acggctgcgt
    11161 cagagcatcc aatagtctgc tgtgcccaat cgtatccaaa cagtctttcg acccaagcgg
    11221 ctggtgaccc ggcgtgcagt ccgtcctgct caatcatgat atcaggaccg gggttttctt
    11281 ccacgtctcc tgcttgcttt aacagagaga agttcgtggc accagagcca gatctctcga
    11341 ggccctgtgg gaggaagaga agaggtcaga agcttgacgc gtgccccact gtggggtgga
    11401 ggggacagat aaaagtaccc agaaccagag ccacattaac cggccctggg aatataaggt
    11461 ggtcccagct cggggacaca ggatccctgg aggcagcaaa catgctgtcc tgaagtggac
    11521 ataggggccc gggttggagg aagaagacta gctgagctct cggacccctg gaagatgcca
    11581 tgacaggggg ctggaagagc tagcacagac tagagaggta aggggggtag gggagctgcc
    11641 caaatgaaag gagtgagagg tgacccgaat ccacaggaga acggggtgtc caggcaaaga
    11701 aagcaagagg atggagaggt ggctaaagcc agggagacgg ggtactttgg ggttgtccag
    11761 aaaaacggtg atgatgcagg cctacaagaa ggggaggcgg gacgcaaggg agacatccgt
    11821 cggagaaggc catcctaaga aacgagagat ggcacaggcc ccagaaggag aaggaaaagg
    11881 gaacccagcg agtgaagacg gcatggggtt gggtgaggga ggagagatgc ccggagagga
    11941 cccagacacg gggaggatcc gctcagagga catcacgtgg tgcagcgccg agaaggaagt
    12001 gctccggaaa gagcatcctt gggcagcaac acagcagaga gcaaggggaa gagggagtgg
    12061 aggaagacgg aacctgaagg aggcggcagg gaaggatctg ggccagccgt agaggtgacc
    12121 caggccacaa gctgcagaca gaaagcggca caggcccagg ggagagaatg ctggtcagag
    12181 aaagcaggta ccaagggcga attccagcac actggcggcc gttactagtg gccgtggagg
    12241 ccttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
    12301 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
    12361 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
    12421 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cctcataaaa
    12481 gttttgttac tttatagaag aaattttgag tttttgtttt ttttaataaa taaataaaca
    12541 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
    12601 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
    12661 tttacacatg attatcttta acgtacgtca caatatgatt atctttctag ggttaatcta
    12721 gctgcgtgtt ctgcagcgtg tcgagcatct tcatctgctc catcacgctg taaaacacat
    12781 ttgcaccgcg agtctgcccg tcctccacgg gttcaaaaac gtgaatgaac gaggcgcgct
    12841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    12901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    12961 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    13021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    13081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    13141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    13201 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    13261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    13321 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    13381 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    13441 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    13501 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    13561 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    13621 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    13681 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    13741 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    13801 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    13861 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    13921 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    13981 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    14041 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    14101 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    14161 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    14221 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    14281 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    14341 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    14401 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    14461 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    14521 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    14581 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    14641 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    14701 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    14761 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    14821 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    14881 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    14941 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    15001 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    15061 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    15121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    15181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    15241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    15301 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    15361 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    15421 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    15481 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    15541 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    15601 ctatgaccat gattacgcca agcgcgcccg ccgggtaact cacggggtat ccatgtccat
    15661 ttctgcggca tccagccagg atacccgtcc tcgctgacgt aatatcccag cgccgcaccg
    15721 ctgtcattaa tctgcacacc ggcacggcag ttccggctgt cgccggtatt gttcgggttg
    15781 ctgatgcgct tcgggctgac catccggaac tgtgtccgga aaagccgcga cgaactggta
    15841 tcccaggtgg cctgaacgaa cagttcaccg ttaaaggcgt gcatggccac accttcccga
    15901 atcatcatgg taaacgtgcg ttttcgctca acgtcaatgc agcagcagtc atcctcggca
    15961 aactctttcc atgccgcttc aacctcgcgg gaaaaggcac gggcttcttc ctccccgatg
    16021 cccagatagc gccagcttgg gcgatgactg agccggaaaa aagacccgac gatatgatcc
    16081 tgatgcagct agattaaccc tagaaagata gtctgcgtaa aattgacgca tgcatctcga
    16141 gagagcagag ccaggaacac ctgtagggaa ggggcaggag agccaggggc atgagatggt
    16201 ggacgaggaa gggggacagg gaagcctgag cgcctctcct gggcttgcca aggactcaaa
    16261 cccagaagcc cagagcaggg ccttagggaa gcgggaccct gctctgggcg gaggaatatg
    16321 tcccagatag cactggggac tctttaagga aagaaggatg gagaaagaga aagggagtag
    16381 aggcggccac gacctggtga acacctagga cgcaccattc tcacaaaggg agttttccac
    16441 acggacaccc ccctcctcac cacagccctg ccaggacggg gctggctact ggccttatct
    16501 cacaggtaaa actgacgcac ggaggaacaa tataaattgg ggactagaaa ggtgaagagc
    16561 caaagttaga actcaggacc aacttattct gattttgttt ttccaaactg cttctcctct
    16621 tgggaagtgt aaggaagctg cagcaccagg atcagtgaaa cgcaccagac agccgcgtca
    16681 gagcagctca ggttctggga gagggtagcg cagggtggcc actgagaacc gggcaggtca
    16741 cgcatccccc ccttccctcc caccccctgc caagctctcc ctcccaggat cctctctggc
    16801 tccatcgtaa gcaaacctta gaggttctgg caaggagaga gatggctcca ggaaatgggg
    16861 gtgtgtcacc agataaggaa tctgcctaac aggaggtggg ggttagaccc aatatcagga
    16921 gactaggaag gaggaggcct aaggatgggg cttttctgtc accaatcctg tccctagtag
    16981 tgtacatgcc agaacatttc tccc
//
