LOCUS       sequence-271983-       17159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for integration at the human AAVS1 safe harbor locus of
            Doxicycline-inducible dCas9-VPR-T2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D,
            Eggan K, Barrett LE
  TITLE     A multiplexed gRNA piggyBac transposon system facilitates efficient 
            induction of CRISPRi and CRISPRa in human pluripotent stem cells.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
  PUBMED    31959800
REFERENCE   2  (bases 1 to 17159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):635. doi"; pages: " 
            10.1038/s41598-020-57500-1"
COMMENT     Sequence Label: pT076
FEATURES             Location/Qualifiers
     source          1..17159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          551..1568
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1492..1514
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1576..1595
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1634..2383
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPGS"
     polyA_signal    2392..2616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2931..2950
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2949..3004
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3050..3069
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3957..4013)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4037..4062
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    4076..4879
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     repeat_region   5343..5405
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5535..5551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5544..5566)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5693..6148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5780..5799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5990..6011
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6174..6278
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6279..6347,6348..7139)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6497..6516)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7310..7898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7799..7818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8052..8069
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8186..8207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8222..8227,8228..8245,8246..8252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8260..8276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8265..8287
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8284..8300
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8284..8300
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8791..8825
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    8835..9671
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        9700..10067
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    9708..9726
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9744..9762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9780..9798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9816..9834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9852..9870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9888..9906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9924..9942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      10073..10082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10082..10147
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10175..10195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10196..10219,10220..10222,10223..12709,12710..12712,
                     12713..14296)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             14309..14329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    14366..14390
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             14420..14569
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             14594..14614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15044..15400
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             15419..15988
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             15989..16042
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(16055..16057,16058..16060,16061..16771)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(16055..16057,16058..16060,16061..16771)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(16361..16380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16708..16729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             16781..16807
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    16817..17041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    17042..17075
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggcgggg
      421 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      481 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      541 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      601 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      661 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
      721 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
      781 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
      841 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
      901 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
      961 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1021 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1081 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1141 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1201 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1261 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1321 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1381 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1441 gggctgtccg cggggggacg gctgccttcg gggggggacg gggcagggcg gggttcggct
     1501 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     1561 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1621 tgcggccgcc accatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     1681 actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     1741 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     1801 aatcgagatg ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca
     1861 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     1921 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     1981 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     2041 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     2101 ggaaagagag acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct
     2161 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     2221 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     2281 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     2341 cgctcttgac gattttgacc ttgacatgct ccccgggtca taggtttaaa cctgtgcctt
     2401 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2461 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2521 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2581 atagcaggca tgctggggat gcggtgggct ctatggctta aggatctcca taagagaaga
     2641 gggacagcta tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacaggt
     2701 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
     2761 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
     2821 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
     2881 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     2941 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     3001 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     3061 ggaaaaagat cacagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
     3121 tgataggcag cctgcacctg aggagtgtta acctattaaa agaactcatc cagcagtctg
     3181 taaaaagcaa tcctctgtga atcaggtgcg gcaatcccat acagcaccag gaacctatcg
     3241 gcccattccc cgcccagttc ctctgcaatg tctcttgtgg ccagggcgat gtcctggtat
     3301 ctgtcggcga cgcccagtcg tccacaatca ataaacccac tgaatctgcc attctccacc
     3361 atgatgttgg gcaggcaagc gtcgccatgg gtgaccacca gatcttctcc gtctggcatt
     3421 ctggctttca gccgtgcaaa cagctctgca ggagccagcc cctggtgttc ctcatccaga
     3481 tcgtcctggt ccaccagccc ggcctccatt cgggttcgag ccctctcaat gcgatgttta
     3541 gcctggtgat cgaaagggca ggtggcgggg tccagagtat gcagtctccg catggcatct
     3601 gccatgatgg agaccttctc agctggtgcc aggtgggagc tcagcaggtc ctgtcctggc
     3661 acttctccca gcagcagcca atctcgcccg gcctcagtga ccacgtccag gactgcagca
     3721 caaggcactc ctgtggtggc cagccagctc aggcgggctg cttcatcctg cagctcgttc
     3781 agtgctcctg acaggtctgt cttgacaaac agcacggggc gcccctgagc actcagccgg
     3841 aacacggctg cgtcagagca tccaatagtc tgctgtgccc aatcgtatcc aaacagtctt
     3901 tcgacccaag cggctggtga cccggcgtgc agtccgtcct gctcaatcat gatatcagga
     3961 ccggggtttt cttccacgtc tcctgcttgc tttaacagag agaagttcgt ggcaccagag
     4021 ccagatctct cgaggccctg tgggaggaag agaagaggtc agaagcttga cgcgtgcccc
     4081 actgtggggt ggaggggaca gataaaagta cccagaacca gagccacatt aaccggccct
     4141 gggaatataa ggtggtccca gctcggggac acaggatccc tggaggcagc aaacatgctg
     4201 tcctgaagtg gacatagggg cccgggttgg aggaagaaga ctagctgagc tctcggaccc
     4261 ctggaagatg ccatgacagg gggctggaag agctagcaca gactagagag gtaagggggg
     4321 taggggagct gcccaaatga aaggagtgag aggtgacccg aatccacagg agaacggggt
     4381 gtccaggcaa agaaagcaag aggatggaga ggtggctaaa gccagggaga cggggtactt
     4441 tggggttgtc cagaaaaacg gtgatgatgc aggcctacaa gaaggggagg cgggacgcaa
     4501 gggagacatc cgtcggagaa ggccatccta agaaacgaga gatggcacag gccccagaag
     4561 gagaaggaaa agggaaccca gcgagtgaag acggcatggg gttgggtgag ggaggagaga
     4621 tgcccggaga ggacccagac acggggagga tccgctcaga ggacatcacg tggtgcagcg
     4681 ccgagaagga agtgctccgg aaagagcatc cttgggcagc aacacagcag agagcaaggg
     4741 gaagagggag tggaggaaga cggaacctga aggaggcggc agggaaggat ctgggccagc
     4801 cgtagaggtg acccaggcca caagctgcag acagaaagcg gcacaggccc aggggagaga
     4861 atgctggtca gagaaagcag gtaccaaggg cgaattccag cacactggcg gccgttacta
     4921 gtggccgtgg aggccttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     4981 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     5041 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     5101 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     5161 gtccctcata aaagttttgt tactttatag aagaaatttt gagtttttgt tttttttaat
     5221 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     5281 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     5341 cacatgcgtc aattttacac atgattatct ttaacgtacg tcacaatatg attatctttc
     5401 tagggttaat ctagctgcgt gttctgcagc gtgtcgagca tcttcatctg ctccatcacg
     5461 ctgtaaaaca catttgcacc gcgagtctgc ccgtcctcca cgggttcaaa aacgtgaatg
     5521 aacgaggcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5581 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5641 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5821 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6061 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6121 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6781 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6841 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6901 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6961 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7021 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7081 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7141 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8101 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8161 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8221 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8281 acacaggaaa cagctatgac catgattacg ccaagcgcgc ccgccgggta actcacgggg
     8341 tatccatgtc catttctgcg gcatccagcc aggatacccg tcctcgctga cgtaatatcc
     8401 cagcgccgca ccgctgtcat taatctgcac accggcacgg cagttccggc tgtcgccggt
     8461 attgttcggg ttgctgatgc gcttcgggct gaccatccgg aactgtgtcc ggaaaagccg
     8521 cgacgaactg gtatcccagg tggcctgaac gaacagttca ccgttaaagg cgtgcatggc
     8581 cacaccttcc cgaatcatca tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcagca
     8641 gtcatcctcg gcaaactctt tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc
     8701 ttcctccccg atgcccagat agcgccagct tgggcgatga ctgagccgga aaaaagaccc
     8761 gacgatatga tcctgatgca gctagattaa ccctagaaag atagtctgcg taaaattgac
     8821 gcatgcatct cgagagagca gagccaggaa cacctgtagg gaaggggcag gagagccagg
     8881 ggcatgagat ggtggacgag gaagggggac agggaagcct gagcgcctct cctgggcttg
     8941 ccaaggactc aaacccagaa gcccagagca gggccttagg gaagcgggac cctgctctgg
     9001 gcggaggaat atgtcccaga tagcactggg gactctttaa ggaaagaagg atggagaaag
     9061 agaaagggag tagaggcggc cacgacctgg tgaacaccta ggacgcacca ttctcacaaa
     9121 gggagttttc cacacggaca cccccctcct caccacagcc ctgccaggac ggggctggct
     9181 actggcctta tctcacaggt aaaactgacg cacggaggaa caatataaat tggggactag
     9241 aaaggtgaag agccaaagtt agaactcagg accaacttat tctgattttg tttttccaaa
     9301 ctgcttctcc tcttgggaag tgtaaggaag ctgcagcacc aggatcagtg aaacgcacca
     9361 gacagccgcg tcagagcagc tcaggttctg ggagagggta gcgcagggtg gccactgaga
     9421 accgggcagg tcacgcatcc cccccttccc tcccaccccc tgccaagctc tccctcccag
     9481 gatcctctct ggctccatcg taagcaaacc ttagaggttc tggcaaggag agagatggct
     9541 ccaggaaatg ggggtgtgtc accagataag gaatctgcct aacaggaggt gggggttaga
     9601 cccaatatca ggagactagg aaggaggagg cctaaggatg gggcttttct gtcaccaatc
     9661 ctgtccctag tagtgtacat gccagaacat ttctccctcg agtttactcc ctatcagtga
     9721 tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact
     9781 ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat
     9841 gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga
     9901 tagagaacgt atatccagtt tactccctat cagtgataga gaacgtataa gctttgctta
     9961 tgtaaaccag ggcgcctata aaagagtgct gattttttga gtaaacttca attccacaac
    10021 acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaagcc gggccaccat
    10081 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
    10141 cgataagggt atccacggag tcccagcagc cgctcctaag aaaaagcgga aggtggacaa
    10201 gaaatactca atcgggctgg ccatcggaac taactcagtg gggtgggcag tcattactga
    10261 cgagtacaaa gtgccaagca agaaatttaa ggtcctgggc aacaccgata ggcactccat
    10321 caagaaaaat ctgattgggg ccctgctgtt cgactctgga gagacagctg aagcaactag
    10381 actgaaaagg actgctagaa ggcgctatac ccggcgaaag aatcgcatct gctacctgca
    10441 ggagattttc tctaacgaaa tggccaaggt ggacgatagt ttctttcatc ggctggagga
    10501 atcattcctg gtcgaggaag ataagaaaca cgagagacat cctatctttg gaaacattgt
    10561 ggacgaggtc gcttatcacg aaaaataccc caccatctat catctgcgca agaaactggt
    10621 ggactctaca gataaagcag acctgcggct gatctatctg gccctggctc acatgattaa
    10681 gttcagaggc cattttctga tcgagggaga tctgaaccca gacaatagcg atgtggacaa
    10741 gctgttcatc cagctggtcc agacatacaa tcagctgttt gaggaaaacc ctattaatgc
    10801 atctggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
    10861 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
    10921 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
    10981 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
    11041 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
    11101 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
    11161 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
    11221 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
    11281 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
    11341 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
    11401 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
    11461 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
    11521 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
    11581 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
    11641 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
    11701 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
    11761 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
    11821 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
    11881 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
    11941 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
    12001 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
    12061 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
    12121 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
    12181 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
    12241 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
    12301 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
    12361 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
    12421 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
    12481 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
    12541 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
    12601 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
    12661 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
    12721 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
    12781 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
    12841 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
    12901 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
    12961 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
    13021 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
    13081 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
    13141 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
    13201 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
    13261 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
    13321 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
    13381 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
    13441 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
    13501 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
    13561 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
    13621 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
    13681 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
    13741 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
    13801 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
    13861 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
    13921 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
    13981 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
    14041 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
    14101 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
    14161 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
    14221 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
    14281 tcagctggga ggcgacagca gggctgaccc caagaagaag aggaaggtgt cgccagggat
    14341 ccgtcgactt gacgcgttga tatcaacaag tttgtacaaa aaagcaggct acaaagaggc
    14401 cagcggttcc ggacgggctg acgcattgga cgattttgat ctggatatgc tgggaagtga
    14461 cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg actttgacct
    14521 cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctga ttaactctag
    14581 aagttccgga tctccgaaaa agaaacgcaa agttggtagc cagtacctgc ccgacaccga
    14641 cgaccggcac cggatcgagg aaaagcggaa gcggacctac gagacattca agagcatcat
    14701 gaagaagtcc cccttcagcg gccccaccga ccctagacct ccacctagaa gaatcgccgt
    14761 gcccagcaga tccagcgcca gcgtgccaaa acctgccccc cagccttacc ccttcaccag
    14821 cagcctgagc accatcaact acgacgagtt ccctaccatg gtgttcccca gcggccagat
    14881 ctctcaggcc tctgctctgg ctccagcccc tcctcaggtg ctgcctcagg ctcctgctcc
    14941 tgcaccagct ccagccatgg tgtctgcact ggctcaggca ccagcacccg tgcctgtgct
    15001 ggctcctgga cctccacagg ctgtggctcc accagcccct aaacctacac aggccggcga
    15061 gggcacactg tctgaagctc tgctgcagct gcagttcgac gacgaggatc tgggagccct
    15121 gctgggaaac agcaccgatc ctgccgtgtt caccgacctg gccagcgtgg acaacagcga
    15181 gttccagcag ctgctgaacc agggcatccc tgtggcccct cacaccaccg agcccatgct
    15241 gatggaatac cccgaggcca tcacccggct cgtgacaggc gctcagaggc ctcctgatcc
    15301 agctcctgcc cctctgggag caccaggcct gcctaatgga ctgctgtctg gcgacgagga
    15361 cttcagctct atcgccgata tggatttctc agccttgctg ggctctggca gcggcagccg
    15421 ggattccagg gaagggatgt ttttgccgaa gcctgaggcc ggctccgcta ttagtgacgt
    15481 gtttgagggc cgcgaggtgt gccagccaaa acgaatccgg ccatttcatc ctccaggaag
    15541 tccatgggcc aaccgcccac tccccgccag cctcgcacca acaccaaccg gtccagtaca
    15601 tgagccagtc gggtcactga ccccggcacc agtccctcag ccactggatc cagcgcccgc
    15661 agtgactccc gaggccagtc acctgttgga ggatcccgat gaagagacga gccaggctgt
    15721 caaagccctt cgggagatgg ccgatactgt gattccccag aaggaagagg ctgcaatctg
    15781 tggccaaatg gacctttccc atccgccccc aaggggccat ctggatgagc tgacaaccac
    15841 acttgagtcc atgaccgagg atctgaacct ggactcaccc ctgaccccgg aattgaacga
    15901 gattctggat accttcctga acgacgagtg cctcttgcat gccatgcata tcagcacagg
    15961 actgtccatc ttcgacacat ctctgtttga gggcagagga agtctgctaa catgcggtga
    16021 cgtcgaggag aatcctggcc cagcaccggg atccatggtg agcaagggcg aggagctgtt
    16081 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
    16141 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
    16201 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
    16261 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
    16321 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
    16381 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
    16441 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
    16501 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
    16561 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
    16621 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
    16681 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
    16741 gatcactctc ggcatggacg agctgtacaa gggctccggg cccgccgcca agagggtgaa
    16801 gctcgactag tagtaactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    16861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    16921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    16981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
    17041 gataacttcg tataatgtat gctatacgaa gttatgcccg ggcaaggatc tgcgatcgct
    17101 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccg
//
