LOCUS       sequence-272001-        4687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - CGB Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pCGB
FEATURES             Location/Qualifiers
     source          1..4687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1852..1870)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1853..1870)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1888..1904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1888..1904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1901..1923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1912..1928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1936..1942,1943..1960,1961..1966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2119..2136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2290..2878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2370..2389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3049..3840,3841..3909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3672..3691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3910..4014)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4092..4547)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4229..4250)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4441..4460
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4673..4687,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4687,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgggcgatga gtggtctcga
      121 atggtgaaac gggagaagaa cgtcatctat ggacctgagc cactccatcc acttgaggac
      181 ctcactgccg gagaaatgct gtttcgggct ctccggaaac actcacattt gcctcaagcc
      241 cttgtggacg tagtagggga tgaaagtctc tcctataaag aatttttcga agctacggtg
      301 ctgttggcgc aatctctcca taactgtggg tacaagatga acgacgtcgt gtccatttgt
      361 gccgagaaca acacacggtt ctttattccc gtaatcgctg cgtggtacat cgggatgatt
      421 gtggcacccg tcaacgaaag ttatatccca gatgaacttt gcaaagtaat ggggatcagc
      481 aagccacaga ttgtttttac caccaagaac atcttgaata aagtattgga agtacagtct
      541 cgcacaaatt tcataaagcg gatcataata ttggatacag tcgaaaacat ccatggctgc
      601 gaatcattgc ctaacttcat aagtagatac tcagatggaa atattgcgaa cttcaaacct
      661 ctccatttcg accctgttga gcaagtcgct gccattctgt gctcaagtgg gacaacgggg
      721 ctcccaaagg gggtgatgca gactcatcaa aacatatgtg tgcgcctgat acacgcgttg
      781 gatcctagag taggcaccca actcatccca ggcgttactg tgctggtata tttgcccttt
      841 ttccatgcat ttggcttctc aatcacattg ggctacttca tggtaggcct gagagtcatc
      901 atgtttcggc ggtttgatca ggaagccttt ctcaaggcca ttcaggatta tgaagtacga
      961 tctgttataa acgtcccatc cgtcattctt tttttgtcca aatcccctct cgtggacaaa
     1021 tacgatcttt catccctgcg ggaactttgt tgcggcgccg caccactcgc caaggaagta
     1081 gctgaagtcg ccgcaaaaag acttaacctt cccgggattc ggtgtgggtt tggccttacc
     1141 gagtccacat ctgcaaatat ccactctctt cgggacgaat tcaagagcgg aagtcttggg
     1201 cgagttacgc ccctgatggc agcgaaaata gcggataggg aaactggcaa ggcgctcggt
     1261 cctaatcaag tcggagaact ttgcattaaa ggaccgatgg tgtccaaagg atacgtcaac
     1321 aacgttgaag ctaccaagga agccattgat gatgacggat ggctgcacag cggcgatttc
     1381 gggtattacg acgaggacga acatttctac gttgtggaca gatataaaga acttataaag
     1441 tataaaggga ctcaagtagc tcccgctgag ttggaagaaa tcctcttgaa gaatccgtgc
     1501 ataagagatg ttgctgttgt gggcatcccc gacctggaag caggggaact tccgagcgcg
     1561 tttgttgtta agcagccggg aaaggagatt actgctaagg aagtctatga ctatctggcc
     1621 gaaagagtga gccatactaa atatctccgg gggggagtcc gcttcgtgga cagcatccct
     1681 aggaatgtga ctggaaaaat aacgaggaaa gaactgctta aacagttgct cgagaaagca
     1741 ggcgggtgag cttcgagacc actcatcgcc caatcactag tgaattcgcg gccgcctgca
     1801 ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
     1861 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2701 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3181 gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4021 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4081 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4141 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4201 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4261 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgctggcc cactacgtga
     4321 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4381 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4441 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4501 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     4561 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     4621 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     4681 cgacgtt
//
