LOCUS       sequence-272004-        3004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - MetLuc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pMetLuc
FEATURES             Location/Qualifiers
     source          1..3004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(286..304)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(287..304)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(320..336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(320..336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(451..1039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(531..550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1154..1256
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1257..1916
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1323..1342)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2029..2484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2116..2135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2326..2347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2503..2520
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2504..2520
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2527..2546
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2527..2545
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caggaatagt gggtgctata gtagatatac cagaaattag cggatttaaa gagatggcac
       61 caatggaaca attcattgct caagtagacc gatgcgccag ctgcacgacg ggctgtttga
      121 agggattggc aaatgttaag tgtagcgaac tcctgaaaaa atggctgcct gaccgctgtg
      181 cctccttcgc ggataagata cagaaggagg tccataatat caagggcatg gcaggagata
      241 ggtaggcttc gagaccactc atcgcccaat cactagtgaa ttcgttctat agtgtcacct
      301 aaataccata gagccgttgg tcatagctgt ttcctgtcca ggcatcatga cacggttatc
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1141 gatccttttg tgatgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     1201 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     1261 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     1321 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     1381 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     1441 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     1501 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     1561 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     1621 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     1681 tgtttttcgt gtcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     1741 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     1801 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     1861 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     1921 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     1981 agcggatgaa tggcagaaat tcgatgataa gctgtcaaac atgatgttac gcgccctgta
     2041 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2101 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2161 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2221 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca gcgccctgat
     2281 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2341 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2401 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2461 acaaaatatt aacgcttaca attttaacat tgtagaaacg catgtaaaac gacggccagt
     2521 gaattgtaat acgactcact atagggtgaa ttccgcttag gaccttagat attgatctca
     2581 aggtctcgaa tggacatcaa agtcgtgttc acgctggtgt tttccgcgct cgtacaagct
     2641 aaatccacag aatttgaccc gaacattgat attgtcggcc tggagggaaa attcggtata
     2701 accaacttgg agacagatct ctttacgatt tgggagacaa tggaggtcat gataaaggcg
     2761 gacatagcag atacagatag ggcaagtaat tttgtagcaa ccgaaacgga cgctaaccgt
     2821 ggcaaaatgc ccggaaagaa gctccctttg gccgtaataa tggagatgga ggctaatgcc
     2881 tttaaagccg gatgtactcg tggctgcctg atttgcttga gcaagatcaa atgtacagcc
     2941 aaaatgaagg tgtatatacc cggacggtgt cacgactatg gcggagataa aaagacggga
     3001 cagg
//
