LOCUS       sequence-272005-        3706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of GLuc in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:GLuc:bgH
FEATURES             Location/Qualifiers
     source          1..3706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      132..587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(219..238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     429..450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        668..971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        972..1175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1126..1146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1768..1975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2103..2691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2183..2202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2806..2908
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(2909..3706,1..18)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             join(2909..3706,1..18)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2982..3001)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctcgatgagt ttttctgatt ttttttaagg cagttattgg tgcccttaaa cgcctggttg
       61 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga taagctgtca
      121 aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      181 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      241 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      301 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      361 acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      421 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      481 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      541 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattttaa cgaattcgtc
      601 tcaggagtag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      661 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      721 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      781 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      841 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      901 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      961 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     1021 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     1081 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1141 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcaagc
     1201 tcaaatgggt gttaaggtgc tgttcgccct gatctgcatt gccgtcgccg aggccaagcc
     1261 cactgagaac aacgaggact tcaacatcgt ggccgttgcc tccaacttcg ccaccactga
     1321 tctggatgcc gaccgcggca agttgcccgg caagaagctg cccctggagg tgctcaagga
     1381 gctggaggcc aacgcccgca aggctggatg cacccgtgga tgcctgatct gcctgtccca
     1441 catcaagtgc acccccaaga tgaagaagtt cattccaggc cgttgccaca cctacgaggg
     1501 cgacaaggag tccgcccagg gcggtattgg cgaggctatc gtcgatatcc cggagatccc
     1561 cggtttcaag gacctggagc ccttggagca gttcattgcc caggttgacc tctgcgtcga
     1621 ctgcaccacc ggctgcctca agggactggc caacgtccag tgctccgacc tgctgaagaa
     1681 gtggctgcca cagcgttgcg ccaccttcgc ttccaaaatc cagggccagg tcgataagat
     1741 caagggtgct ggaggcgact aaggcttctg tgccttctag ttgccagcca tctgttgttt
     1801 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1861 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1921 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc
     1981 gctgtcatga gacgaattct gacacggtta tccacagaat caggggataa cgcaggaaag
     2041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgtgatgatc ggcacgtaag
     2821 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     2881 gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc
     2941 aaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     3001 taatgtcggg caatcaggtg cgacaatcta ccgattgtat gggaagcccg atgcgccaga
     3061 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     3121 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     3181 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga
     3241 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     3301 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gactcgctca
     3361 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     3421 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     3481 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     3541 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     3601 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     3661 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatg
//
