LOCUS       sequence-272012-        3800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible middle entry clone contains human full length 
            NFR2F2 gene with stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pME-NR2F2
FEATURES             Location/Qualifiers
     source          1..3800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(271..298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(390..476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     525..547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     539..556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     540..556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    572..671
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1559..1576
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(1928..2027)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2044..2063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2045..2063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2068..2084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2068..2084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2186..2205)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2197..3006
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2264..2283)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3153..3741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3642..3661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
      241 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      301 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      361 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      421 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
      481 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
      541 taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt
      601 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      661 aaaagcaggc tctccgccac catggcaatg gtagtcagca cgtggcgcga cccccaggac
      721 gaggtgcccg gctcacaggg cagccaggcc tcgcaggcgc cgcccgtgcc cggcccgccg
      781 cccggcgccc cgcacacgcc acagacgccc ggccaagggg gcccagccag cacgccagcc
      841 cagacggcgg ccggtggcca gggcggccct ggcggcccgg gtagcgacaa gcagcagcag
      901 cagcaacaca tcgagtgcgt ggtgtgcgga gacaagtcga gcggcaagca ctacggccag
      961 ttcacgtgcg agggctgcaa gagcttcttc aagcgcagcg tgcggaggaa cctgagctac
     1021 acgtgccgcg ccaaccggaa ctgtcccatc gaccagcacc atcgcaacca gtgccagtac
     1081 tgccgcctca aaaagtgcct caaagtgggc atgagacggg aagcggtgca gaggggcagg
     1141 atgccgccga cccagccgac ccacgggcag ttcgcgctga ccaacgggga tcccctcaac
     1201 tgccactcgt acctgtccgg atatatttcc ctgctgttgc gcgcggagcc ctatcccacg
     1261 tcgcgcttcg gcagccaatg catgcagccc aacaacatca tgggtatcga gaacatttgc
     1321 gaactggccg cgaggatgct cttcagcgcc gtcgagtggg cccggaacat ccccttcttc
     1381 cccgacctgc agatcacgga ccaggtggcc ctgcttcgcc tcacctggag cgagctgttt
     1441 gtgttgaatg cggcgcagtg ctccatgccc ctccacgtcg ccccgctcct ggccgccgcc
     1501 ggcctgcatg cttcgcccat gtccgccgac cgggtggtcg cctttatgga ccacatacgg
     1561 atcttccaag agcaagtgga gaagctcaag gcgctgcacg ttgactcagc cgagtacagc
     1621 tgcctcaagg ccatagtcct gttcacctca gatgcctgtg gtctctctga tgtagcccat
     1681 gtggaaagct tgcaggaaaa gtctcagtgt gctttggaag aatacgttag gagccagtac
     1741 cccaaccagc cgacgagatt cggaaagctt ttgcttcgcc tcccttccct ccgcaccgtc
     1801 tcctcctcag tcatagagca attgtttttc gtccgtttgg taggtaaaac ccccatcgaa
     1861 accctcatcc gggatatgtt actgtccggc agcagtttta actggccgta tatggcaatt
     1921 caataacacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1981 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2041 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2101 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2161 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2221 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2281 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2341 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2401 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2461 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2521 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2581 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2641 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2701 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2761 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2821 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2881 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2941 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3001 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3061 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3781 tgctggcctt ttgctcacat
//
