LOCUS       sequence-272014-       10985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains 161k modified human enhancer with with 
            NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla
            Luciferase driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
FEATURES             Location/Qualifiers
     source          1..10985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2168..2188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3180..3204
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3211..3242
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3269..3278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3275..4927
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    complement(4941..4965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4966..5717
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          5787..5919
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5902..5921
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5964..5982
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5992..6927
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(6975..6995)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7010..7598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7063..7083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7481..7492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7685..7918
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7990..8111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8027..8046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8081..8100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8151..8286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8307..8325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8335..8352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8335..8351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8493..8948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8580..8599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8790..8811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8974..9078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9079..9147,9148..9939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9297..9316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10110..10698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10599..10618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10852..10869
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2101 atcgatcacg agactagcct cctagcccgg gctcgagcag ctgaagcttg catgcctgca
     2161 gggctatcaa ctttgtatag aaaagttggg ctgctgattc atatcccagt gaggtcatct
     2221 gaattttggg ggacctcaaa tagaaatcat atgggggaaa gaatgagcct agctcaaacc
     2281 agacaggctg agagcttatc ttgccaggac ataggctccc cggtgggtgg gggagatggc
     2341 cattgctgcc tttctttcct tccccactga atttaagcag atgcagttag tcactgtgca
     2401 aaggagtatg tgcaaatcta agtattcatg tttatgtgtg gttgtcaggt ctaaatcagc
     2461 caggcagtaa tttctctggg agcccaatgt aggcactaaa ttttgcactc aaccaccgaa
     2521 tggtccacat gccccttaat ctgtcttcct gcaggagggc tcccttggca ccatcctgcc
     2581 tgaacttggg gccagccttt cactggagcc ccttcccgtg aattatggcc tacagcatgg
     2641 gggctgaata gaaaaaaaaa aaaccctcca gaaattcaac tggaagggct gaccggaatt
     2701 caacctaatg ttgagaatag caggaaagct tctattattc tacttcattc tatctgctat
     2761 gaggcagtgc ggtcgcctgg agtcgactga gcttaactca tttccctgga tttccgagtc
     2821 acagccatgg tgcctgctat agttaacgat cgaactcctg agactcacta cacttcctga
     2881 taggacgtac tcacgtaccg cctagtaaca tcatacgttc ctataggagt cataatatga
     2941 cctagacaag acctaggagc caatcagaat gcaaatgatt gatgggagtg cttttggtta
     3001 tctactgttt gtcctgacgc ttggcgtggt tctttgtatt atgattgtgt gggcagtagg
     3061 cttaaaaata catacaggca ttgctttcca cattgttgaa ggttaatcct aaggtgtggc
     3121 aaagaaacta tattttatag actggaacct catgggcacc agctagtttt tgctcttcct
     3181 caagtttgta caaaaaagca ggctagacac tagagggtat ataatggaag ctcgacttcc
     3241 agcttggcaa tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa
     3301 gggcccagcg ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc
     3361 catgaagcgc tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt
     3421 ggacattacc tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg
     3481 ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt
     3541 catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta
     3601 caacgagcgc gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag
     3661 caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat
     3721 catcatcatg gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac
     3781 ttcccatttg ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga
     3841 caaaaccatc gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc
     3901 cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa
     3961 ccagatcatc cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat
     4021 gttcaccacg ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga
     4081 ggaggagcta ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc
     4141 cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt
     4201 gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa
     4261 acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat
     4321 tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt
     4381 cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga
     4441 gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa
     4501 cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga
     4561 cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt
     4621 agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt
     4681 cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca
     4741 cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc
     4801 caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa
     4861 gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc
     4921 cgtgtaataa ttctagagtc acccagcttt cttgtacaaa gtggaaatga gtcttcggac
     4981 ctcgcggggg ccgcttaagc ggtggttagg gtttgtctga cgcgggggga gggggaagga
     5041 acgaaacact ctcattcgga ggcggctcgg ggtttggtct tggtggccac gggcacgcag
     5101 aagagcgccg cgatcctctt aagcaccccc ccgccctccg tggaggcggg ggtttggtcg
     5161 gcgggtggta actggcgggc cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt
     5221 ttcggtctgc tcgcagaccc ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc
     5281 ctaggctcca tggggaccgt atacgtggac aggctctgga gcatccgcac gactgcggtg
     5341 atattaccgg agaccttctg cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt
     5401 tgggcgacaa acaccaggac ggggcacagg tacactatct tgtcacccgg aggcgcgagg
     5461 gactgcagga gcttcaggga gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt
     5521 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     5581 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     5641 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     5701 tgcagcgacc cgcttaaaag cttgattctt ctgacacaac agtctcgaac ttaagctgca
     5761 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     5821 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     5881 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
     5941 acagctctta aggctagagt acttaatacg actcactata ggctagccac catgacttcg
     6001 aaagtttatg atccagaaca aaggaaacgg atgataactg gtccgcagtg gtgggccaga
     6061 tgtaaacaaa tgaatgttct tgattcattt attaattatt atgattcaga aaaacatgca
     6121 gaaaatgctg ttattttttt acatggtaac gcggcctctt cttatttatg gcgacatgtt
     6181 gtgccacata ttgagccagt agcgcggtgt attataccag accttattgg tatgggcaaa
     6241 tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc attacaaata tcttactgca
     6301 tggtttgaac ttcttaattt accaaagaag atcatttttg tcggccatga ttggggtgct
     6361 tgtttggcat ttcattatag ctatgagcat caagataaga tcaaagcaat agttcacgct
     6421 gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc ctgatattga agaagatatt
     6481 gcgttgatca aatctgaaga aggagaaaaa atggttttgg agaataactt cttcgtggaa
     6541 accatgttgc catcaaaaat catgagaaag ttagaaccag aagaatttgc agcatatctt
     6601 gaaccattca aagagaaagg tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc
     6661 ccgttagtaa aaggtggtaa acctgacgtt gtacaaattg ttaggaatta taatgcttat
     6721 ctacgtgcaa gtgatgattt accaaaaatg tttattgaat cggacccagg attcttttcc
     6781 aatgctattg ttgaaggtgc caagaagttt cctaatactg aatttgtcaa agtaaaaggt
     6841 cttcattttt cgcaagaaga tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt
     6901 gagcgagttc tcaaaaatga acaataattc tagagcggcc gcttcgagca gacatgataa
     6961 gatacattga tgagcaactt tattatacat agttgataat tcactcgaca atcaacctct
     7021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7381 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7561 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct
     7621 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7681 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     7741 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7801 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7861 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7921 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7981 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8101 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     8161 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8221 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8281 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8341 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8401 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8461 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     8521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     8941 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9001 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9061 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9121 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9181 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9241 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9301 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9361 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9421 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9481 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9541 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9601 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9661 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9721 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9781 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9841 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9901 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9961 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10021 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10081 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10861 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10921 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10981 gcaat
//
