LOCUS       sequence-272032-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT(UUA)_FLAG-AcKRS
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2728..2939
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2952..3220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3270..3279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4686..5260,5261..5263,5264..5272)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4853..4870)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5080..5099
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5285..5884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5285..5304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5781..5801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5944..5964)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5999..6120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6036..6055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6090..6109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6882..6916
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7407..7423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7407..7423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7420..7442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7431..7447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7455..7461,7462..7479,7480..7485))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7500..7521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7638..7655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7809..8397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7889..7908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8568..9359,9360..9428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9191..9210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccaac tagttctgca gtatttagca
     1381 tgccccaccc atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt
     1441 ttatctcaaa ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat
     1501 tttagttaaa tttcctgctg aagctctagt acgataagta acttgaccta agtgtaaagt
     1561 tgagatttcc ttcaggttta tatagcttgt gcgccgcctg ggtacctcgg aaacctgatc
     1621 atgtagatcg aacggacttt aaatccgttc agccgggtta gattcccggg gtttccggac
     1681 aagtgcggtt tttcctgcag tatttagcat gccccaccca tctgcaaggc attctggata
     1741 gtgtcaaaac agccggaaat caagtccgtt tatctcaaac tttagcattt tgggaataaa
     1801 tgatatttgc tatgctggtt aaattagatt ttagttaaat ttcctgctga agctctagta
     1861 cgataagtaa cttgacctaa gtgtaaagtt gagatttcct tcaggtttat atagcttgtg
     1921 cgccgcctgg gtacctcgga aacctgatca tgtagatcga acggacttta aatccgttca
     1981 gccgggttag attcccgggg tttccggaca agtgcggttt ttcctgcagt atttagcatg
     2041 ccccacccat ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt
     2101 atctcaaact ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt
     2161 tagttaaatt tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg
     2221 agatttcctt caggtttata tagcttgtgc gccgcctggg tacctcggaa acctgatcat
     2281 gtagatcgaa cggactttaa atccgttcag ccgggttaga ttcccggggt ttccggacaa
     2341 gtgcggtttt tcctgcagta tttagcatgc cccacccatc tgcaaggcat tctggatagt
     2401 gtcaaaacag ccggaaatca agtccgttta tctcaaactt tagcattttg ggaataaatg
     2461 atatttgcta tgctggttaa attagatttt agttaaattt cctgctgaag ctctagtacg
     2521 ataagtaact tgacctaagt gtaaagttga gatttccttc aggtttatat agcttgtgcg
     2581 ccgcctgggt acctcggaaa cctgatcatg tagatcgaac ggactttaaa tccgttcagc
     2641 cgggttagat tcccggggtt tccggacaag tgcggttttt cactagtgct ctagaaagga
     2701 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2761 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     2821 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2881 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     2941 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     3001 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     3061 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     3121 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     3181 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     3241 ctctagagct agcgtttaaa cttaagcttg ccaccatgga ctacaaggac gacgacgaca
     3301 agatggacaa gaagcccctg aacaccctga tcagcgccac aggactgtgg atgtccagaa
     3361 ccggcaccat ccacaagatc aagcaccacg aggtgtcccg gtccaaaatc tacatcgaga
     3421 tggcctgcgg cgatcacctg gtcgtcaaca acagcagaag cagccggaca gccagagccc
     3481 tgcggcacca caagtacaga aagacctgca agcggtgcag agtgtccgac gaggacctga
     3541 acaagttcct gaccaaggcc aacgaggacc agaccagcgt gaaagtgaag gtggtgtccg
     3601 cccccacccg gaccaagaaa gccatgccca agagcgtggc cagagccccc aagcccctgg
     3661 aaaacaccga agccgctcag gcccagccca gcggcagcaa gttcagcccc gccatccccg
     3721 tgtctaccca ggaaagcgtc agcgtccccg ccagcgtgtc caccagcatc tctagcatct
     3781 caaccggcgc cacagcttct gccctggtca agggcaacac caaccccatc accagcatgt
     3841 ctgcccctgt gcaggcctct gccccagccc tgaccaagtc ccagaccgac cggctggaag
     3901 tgctcctgaa ccccaaggac gagatcagcc tgaacagcgg caagcccttc cgggagctgg
     3961 aaagcgagct gctgagccgg cggaagaagg acctccagca aatctacgcc gaggaacggg
     4021 agaactacct gggcaagctg gaaagagaga tcacccggtt cttcgtggac cggggcttcc
     4081 tggaaatcaa gagccccatc ctgatccccc tggagtacat cgagcggatg ggcatcgaca
     4141 acgacaccga gctgagcaag cagattttcc gggtggacaa gaacttctgc ctgcggccca
     4201 tgatggcccc caacatcttc aactacgccc ggaaactgga tcgcgctctg cccgacccca
     4261 tcaagatttt cgagatcggc ccctgctacc ggaaagagag cgacggcaaa gagcacctgg
     4321 aagagtttac aatgctgaac tttttccaga tgggcagcgg ctgcaccaga gagaacctgg
     4381 aatccatcat caccgacttt ctgaaccacc tggggatcga cttcaagatc gtgggcgaca
     4441 gctgcatggt gtacggcgac accctggacg tgatgcacgg cgacctggaa ctgtctagcg
     4501 ccgtcgtggg acccatccct ctggaccggg agtggggcat cgataagccc tggatcggag
     4561 ccggcttcgg cctggaacgg ctgctgaaag tcaagcacga ctttaagaac atcaagcggg
     4621 ctgccagaag cgagagctac tacaacggca tcagcaccaa cctgtgatga ggatccgcgg
     4681 ccgcgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4741 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     4801 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4861 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4921 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4981 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5041 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5101 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5161 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5221 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggcgtc
     5281 cggaatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     5341 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     5401 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5461 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5521 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5581 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5641 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5701 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5761 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5821 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5881 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5941 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgttaa
     6001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6121 tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctat ctggtctccc
     6181 ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc
     6241 ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt
     6301 ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg
     6361 gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     6421 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     6481 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     6541 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     6601 tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     6661 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     6721 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     6781 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     6841 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     6901 gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg
     6961 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     7021 ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga
     7081 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     7141 taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac
     7201 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     7261 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     7321 cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     7381 cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7561 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7621 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7681 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7801 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7861 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7981 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8221 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8941 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9421 atactcat
//
