LOCUS       sequence-272033-       12212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses epitope-tagged human TET3 V1089M variant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Beck DB, Petracovici A, He C, Moore HW, Louie RJ, Ansar M, Douzgou 
            S, Sithambaram S, Cottrell T, Santos-Cortez RLP, Prijoles EJ, Bend 
            R, Keren B, Mignot C, Nougues MC, Ounap K, Reimand T, Pajusalu S, 
            Zahid M, Saqib MAN, Buratti J, Seaby EG, McWalter K, Telegrafi A, 
            Baldridge D, Shinawi M, Leal SM, Schaefer GB, Stevenson RE, Banka S,
            Bonasio R, Fahrner JA
  TITLE     Delineation of a Human Mendelian Disorder of the DNA Demethylation 
            Machinery: TET3 Deficiency.
  JOURNAL   Am J Hum Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007.
  PUBMED    31928709
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007."
COMMENT     Sequence Label: pINTO-N3::hTET3 V1089M
FEATURES             Location/Qualifiers
     source          1..12212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        363..742
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        743..946
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     897..917
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    948..966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    969..987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     997..1021
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1109..1118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1118..1141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1151..1177
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1187..1192,1193..1216,1217..1252,1253..1276)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             1286..1306
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    4112..4123
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6808..6825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6814..7038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7084..7512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7171..7190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7381..7402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7521..7541)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7565..7775
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7587..7783
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7634..7769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7696..7715
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7831..7878
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7897..8271
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8401..8522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8438..8457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8492..8511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8571..8587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8571..8587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8584..8606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8595..8611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8619..8625,8626..8643,8644..8649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8664..8685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9434..9451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9605..10193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9685..9704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10364..11155,11156..11224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10987..11006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11225..11329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11404..11423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atccaggaca gacttggctc gctggtgggc caggacagcg ggtatgtggg gggtcttccc
       61 aagaacgtta aggaaaagct gctgagcttg aagactttac aaagcgagct attcgaagtt
      121 gaaaaggaat ttcaggtgga gatgtttgag ctagagaaca agtttctgca gaagtacaag
      181 cccatttggg agcagcggtc caggatcatt tcaggacaag agcaacccaa gccggaacag
      241 atcgcaaagg gccaagagat tgtggagtca ctcaatgaga cggagttgca attgcatgaa
      301 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      361 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      421 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      481 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      541 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      601 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      661 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      721 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      781 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      841 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      901 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
      961 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     1021 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     1081 cctccggact ctagcgttta aacttaaggc caccatggac tacaaggatg acgacgataa
     1141 gggcagcgga tacccatacg acgtgcctga ctacgccggc agcgctagcg catggtccca
     1201 tcctcaattc gagaaaggcg gcggatctgg cggtggaagc ggaggctccg cctggagcca
     1261 ccctcagttc gaaaagtccg gcagcgagaa cctgtacttc cagggctcca ccggtagcgg
     1321 taccgagctc ggatccgatg gaggactcga cagccagttt caggtgcccc tggccgtcca
     1381 gccggacctg ccaggccttt atgacttccc tcagcgccag gtgatggtag ggagcttccc
     1441 ggggtctggg ctctccatgg ctgggagtga gtcccaactc cgagggggtg gagatggtcg
     1501 aaagaaacgg aaacggtgtg gtacttgtga gccctgccgg cggctggaaa actgtggcgc
     1561 ttgcactagc tgtaccaacc gccgcacgca ccagatctgc aaactgcgaa aatgtgaggt
     1621 gctgaagaaa aaagtagggc ttctcaagga ggtggaaata aaggctggtg aaggagccgg
     1681 gccgtgggga caaggagcgg ctgtcaagac aggctcagag ctcagcccag ttgatggacc
     1741 tgttccaggt cagatggact cagggccagt gtaccatggg gactcacggc agctaagcgc
     1801 ctcaggggtg ccggtcaatg gtgctagaga gcccgctgga cccagtctgc tggggactgg
     1861 gggtccttgg cgggtagacc aaaagcccga ctgggaggct gccccaggcc cagctcatac
     1921 tgctcgcctg gaagatgccc acgatctggt ggccttttcg gctgtggccg aagctgtgtc
     1981 ctcttatggg gcccttagca cccggctcta tgaaaccttc aaccgtgaga tgagtcgtga
     2041 ggctgggaac aacagcaggg gaccccggcc agggcctgag ggctgctctg ctggcagcga
     2101 agaccttgac acactgcaga cggccctggc cctcgcgcgg catggtatga aaccacccaa
     2161 ctgcaactgc gatggcccag aatgccctga ctacctcgag tggctggagg ggaagatcaa
     2221 gtctgtggtc atggaaggag gggaggagcg gcccaggctc ccagggcctc tgcctcctgg
     2281 tgaggccggc ctcccagcac caagcaccag gccactcctc agctcagagg tgccccagat
     2341 ctctccccaa gagggcctgc ccctgtccca gagtgccctg agcattgcca aggaaaaaaa
     2401 catcagcttg cagaccgcca ttgccattga ggccctcaca cagctctcct ctgccctccc
     2461 gcagccttct cattccaccc cccaggcttc ttgccccctt cctgaggcct tgtcacctcc
     2521 tgcccctttc agatctcccc agtcttacct ccgggctccc tcatggcctg tggttcctcc
     2581 tgaagagcac tcatcttttg ctcctgatag ctctgccttc cctccagcaa ctcctagaac
     2641 tgagttccct gaagcctggg gcactgacac ccctccagca acgccccgga gctcctggcc
     2701 catgcctcgc ccaagccccg atcccatggc tgaactggag cagttgttgg gcagcgccag
     2761 tgattacatc cagtcagtat tcaagcggcc tgaggccctg cctaccaagc ccaaggtcaa
     2821 ggtggaggca ccctcttcct ccccggcccc ggccccatcc cctgtacttc agagggaggc
     2881 tcccacgcca tcctcggagc ccgacaccca ccagaaggcc cagaccgccc tgcagcagca
     2941 cctccaccac aagcgcagcc tcttcctaga acaggtgcac gacacctcct tccctgctcc
     3001 ttcagagcct tctgctcctg gctggtggcc cccaccaagt tcacctgtcc cacggcttcc
     3061 agacagacca cccaaggaga agaagaagaa gctcccaaca ccagctggag gtcccgtggg
     3121 aacggagaaa gctgcccctg ggatcaagcc cagtgtccga aagcccattc agatcaagaa
     3181 gtccaggccc cgggaagcac agcccctctt cccacctgtc cgacagattg tcctggaagg
     3241 gcttaggtcc ccagcctccc aggaagtgca ggctcatcca ccggcccctc tgcctgcctc
     3301 acagggctct gctgtgcccc tgcccccaga accttctctt gcgctatttg cacctagtcc
     3361 ctccagggac agcctgctgc cccctactca ggaaatgagg tcccccagcc ccatgacagc
     3421 cttgcagcca ggctccactg gccctcttcc ccctgccgat gacaagctgg aagagctcat
     3481 ccggcagttt gaggctgaat ttggagatag ctttgggctt cccggccccc cttctgtgcc
     3541 cattcaggac cccgagaacc agcaaacatg tctcccagcc cctgagagcc cctttgctac
     3601 ccgttccccc aagcaaatca agattgagtc ttcgggggct gtgactgtgc tctcaaccac
     3661 ctgcttccat tcagaggagg gaggacagga ggccacaccc accaaggctg agaacccact
     3721 cacacccacc ctcagtggct tcttggagtc acctcttaag tacctggaca cacccaccaa
     3781 gagtctgctg gacacacctg ccaagagagc ccaggccgag ttccccacct gcgattgcgt
     3841 cgaacaaata gtggagaaag atgaaggtcc atattatact cacttgggat ctggccccac
     3901 ggtcgcctct atccgggaac tcatggagga gcggtatgga gagaagggga aagccatccg
     3961 gatcgagaag gtcatctaca cggggaagga gggaaagagc tcccgcggtt gccccattgc
     4021 aaagtgggtg atccgcaggc acacgctgga ggagaagcta ctctgcctgg tgcggcaccg
     4081 ggcaggccac cactgccaga acgctgtgat cgtcatcctc atcctggcct gggagggcat
     4141 tccccgtagc ctcggagaca ccctctacca ggagctcacc gacaccctcc ggaagtatgg
     4201 gaaccccacc agccggagat gcggcctcaa cgatgaccgg acctgcgctt gccaaggcaa
     4261 agaccccaac acctgtggtg cctccttctc ctttggttgt tcctggagca tgtacttcaa
     4321 cggctgcaag tatgctcgga gcaagacacc tcgcaagttc cgcctcgcag gggacaatcc
     4381 caaagaggaa gaagtgctcc ggaagagttt ccaggacctg gccaccgaag tcgctcccct
     4441 gtacaagcga ctggcccctc aggcctatca gaaccaggtg accaacgagg aaatagcgat
     4501 tgactgccgt ctggggctga aggaaggacg gcccttcgcg ggggtcacgg cctgcatgga
     4561 cttctgtgcc cacgcccaca aggaccagca taacctctac aatgggtgca ccatggtctg
     4621 caccctgacc aaggaagaca atcgctgcgt gggcaagatt cccgaggatg agcagctgca
     4681 tgttctcccc ctgtacaaga tggccaacac ggatgagttt ggtagcgagg agaaccagaa
     4741 tgcaaaggtg ggcagcggag ccatccaggt gctcaccgcc ttcccccgcg aggtccgacg
     4801 cctgcccgag cctgccaagt cctgccgcca gcggcagctg gaagccagaa aggcagcagc
     4861 cgagaagaag aagattcaga aggagaagct gagcactccg gagaagatca agcaggaggc
     4921 cctggagctg gcgggcatta cgtcggaccc aggcctgtct ctgaagggtg gattgtccca
     4981 gcaaggcctg aagccctccc tcaaggtgga gccgcagaac cacttcagct ccttcaagta
     5041 cagcggcaac gcggtggtgg agagctactc ggtgctgggc aactgccggc cctccgaccc
     5101 ttacagcatg aacagcgtgt actcctacca ctcctactat gcacagccca gcctgacctc
     5161 cgtcaatggc ttccactcca agtacgctct cccgtctttt agctactatg gctttccatc
     5221 cagcaacccc gtcttcccct ctcagttcct gggtcctggt gcctgggggc atagtggcag
     5281 cagtggcagt tttgagaaga agccagacct ccacgctctg cacaacagcc tgagcccggc
     5341 ctacggtggt gctgagtttg ccgagctgcc cagccaggct gttcccacag acgcccacca
     5401 ccccactcct caccaccagc agcctgcgta cccaggcccc aaggagtatc tgcttcccaa
     5461 ggccccccta ctccactcag tgtccaggga cccctccccc tttgcccaga gctccaactg
     5521 ctacaacaga tccatcaagc aagagccagt agacccgctg acccaggctg agcctgtgcc
     5581 cagagacgct ggcaagatgg gcaagacacc tctgtccgag gtgtctcaga atggaggacc
     5641 cagtcacctt tggggacagt actcaggagg cccaagcatg tcccccaaga ggactaacgg
     5701 tgtgggtggc agctggggtg tgttctcgtc tggggagagt cctgccatcg tccctgacaa
     5761 gctcagttcc tttggggcca gctgcctggc cccttcccac ttcacagatg gccagtgggg
     5821 gctgttcccc ggtgaggggc agcaggcagc ttcccactct ggaggacggc tgcgaggcaa
     5881 accgtggagc ccctgcaagt ttgggaacag cacctcggcc ttggctgggc ccagcctgac
     5941 tgagaagccg tgggcgctgg gggcagggga tttcaactcg gccctgaaag gtagtcctgg
     6001 gttccaagac aagctgtgga accccatgaa aggagaggag ggcaggattc cagccgcagg
     6061 ggccagccag ctggacaggg cctggcagtc ctttggtctg cccctgggat ccagcgagaa
     6121 gctgtttggg gctctgaagt cagaggagaa gctgtgggac cccttcagcc tggaggaggg
     6181 gccggctgag gagcccccca gcaagggagc ggtgaaggag gagaagggcg gtggtggtgc
     6241 ggaggaggaa gaggaggagc tgtggtcgga cagtgaacac aacttcctgg acgagaacat
     6301 cggcggcgtg gccgtggccc cagcccacgg ctccatcctc atcgagtgtg cccggcggga
     6361 gctgcacgcc accacgccgc ttaagaagcc caaccgctgc caccccaccc gcatctcgct
     6421 ggtcttctac cagcacaaga acctcaacca gcccaaccac gggctggccc tctgggaagc
     6481 caagatgaag cagctggcgg agagggcacg ggcacggcag gaggaggctg cccggctggg
     6541 cctgggccag caggaggcca agctctacgg gaagaagcgc aagtgggggg gcactgtggt
     6601 tgctgagccc cagcagaaag agaagaaggg ggtcgtcccc acccggcagg cactggctgt
     6661 gcccacagac tcggcggtca ccgtgtcctc ctatgcctac acgaaggtca ctggccccta
     6721 cagccgctgg atcctgtgaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
     6781 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6841 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6901 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6961 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7021 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7081 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7141 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7201 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7261 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7321 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7381 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7441 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7501 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7741 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7801 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7861 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7921 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7981 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8041 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8101 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8161 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     8221 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8521 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8761 gcggggagag gcggtttgcg tattgggcgc tcttccgcta gtctgcaatg agttcgagtt
     8821 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
     8881 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     8941 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     9001 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     9061 atccctgggg gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct
     9121 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     9181 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     9241 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     9301 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     9361 gggggggctg tccccgtgag ctcccaggcg cgccaactcg gtactcgggg atttaattaa
     9421 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9481 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9541 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9841 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9901 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9961 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10021 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    10081 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    10141 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10501 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11341 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
    11401 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    11461 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    11521 caattgttaa ttaagctagc agtctgcaat gagttcgagt tggcgcgcct gtcattctaa
    11581 atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
    11641 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
    11701 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
    11761 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttggga
    11821 gggggctgtc cccgtgagct cccaggcgcg cctgtcattc taaatctctc tttcagccta
    11881 aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
    11941 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
    12001 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
    12061 ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct gtccccgtga
    12121 gctcccaggc gcgccaactc ggtactcggg gatgctagcg caccatcaac gaggaggaca
    12181 tcttggctaa ccaacccctg ttgttacagt cc
//
